Localization of the (TTAGGG)n telomeric sequence in zebrafish chromosomes

Gornung, Ekaterina; Gabrielli, Ivan; Sola, Luciana

doi:10.1139/g97-098

Cited by 18 publications

(11 citation statements)

References 13 publications

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“…The absence of SATA sequences in interstitial regions of chromosome 1 suggests that, if chromosomal fusion has occurred, the satellite DNA sequences, and possibly the inactive centromeres, must have been eliminated by deletion or mutation. The distribution of (TTAGGG) n sequences among 13 fish species showed that these sequences were primarily localized in the telomeres (Meyne et al 1990;Phillips and Reed 1996;Gornung et al 1998) but some nontelomeric sites have been observed (Reed and Phillips 1995). Pericentric blocks of (TTAGGG) n have been reported in other species such as Chinese hamster (Meyne et al 1990) and a recent study by Abuín et al (1996b) found that the NORs in rainbow trout (O. mykiss) hybridized strongly to a (TTAGGG) n probe.…”

Section: Discussionmentioning

confidence: 92%

Molecular and cytogenetic analysis of the telomeric (TTAGGG) n repetitive sequences in the Nile tilapia, Oreochromis niloticus (Teleostei: Cichlidae)

et al. 2002

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The majority of chromosomes in Oreochromis niloticus, as with most fish karyotyped to date, cannot be individually identified owing to their small size. As a first step in establishing a physical map for this important aquaculture species of tilapia we have analyzed the location of the vertebrate telomeric repeat sequence, (TTAGGG)n, in O. niloticus. Southern blot hybridization analysis and a Bal31 sensitivity assay confirm that the vertebrate telomeric repeat is indeed present at O. niloticus chromosomal ends with repeat tracts extending for 4-10 kb on chromosomal ends in erythrocytes. Fluorescent in situ hybridization revealed that (TTAGGG)n is found not only at telomeres, but also at two interstitial loci on chromosome 1. These data support the hypothesis that chromosome 1, which is significantly larger than all the other chromosomes in the karyotype, was produced by the fusion of three chromosomes and explain the overall reduction of chromosomal number from the ancestral teleost karyotype of 2n=48 to 2n=44 observed in tilapia.

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Section: Discussionmentioning

confidence: 92%

Molecular and cytogenetic analysis of the telomeric (TTAGGG) n repetitive sequences in the Nile tilapia, Oreochromis niloticus (Teleostei: Cichlidae)

et al. 2002

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“…The (TTAGGG) n repeats found at human telomeres are present at the telomeres of every vertebrate so far tested by FISH (Moyzis et al, 1988;Meyne et al, 1989). Fishes which have been examined with this telomere probe are numerous Gornung et al, 1998;Pisano et al, 1999;Ozouf-Costaz et al, in preparation). In T. nigroviridis, all telomeres exhibited strong double signals (Fig.…”

Section: Resultsmentioning

confidence: 99%

Karyotype and chromosome location of characteristic tandem repeats in the pufferfish Tetraodon nigroviridis

Fischer

Ozouf−Costaz

Crollius

et al. 2000

Cytogenet Genome Res

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Karyotype analysis of Tetraodon nigroviridis, a pufferfish of the family Tetraodontidae with a small compact genome (385 Mb) which is currently being investigated in our laboratory, indicates that this species has 2n = 42 chromosomes. The small chromosome size (the largest pair measuring less than 3 μm) has complicated accurate chromosome pairing based on morphology alone. DAPI staining, however, provides a banding-like pattern. Because of quantitative variations of some heterochromatin classes, the chromosome formula can not be established precisely, but is estimated to include approximately 20 meta- or submetacentric chromosomes and 22 subtelocentric chromosomes. A centromeric satellite, telomeric repeats, and the major and minor rRNA clusters have been localized unequivocally by FISH. As a result, the 28S and 5S rDNA sequences can be used as chromosome-specific probes.

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“…Among actinopterygians, chromosomal location of the telomeric DNA repeats has been examined in the Chondrostei species from 2 orders: Acipenseriformes [Fontana et al, 1998[Fontana et al, , 2004[Fontana et al, , 2008 and Polypteriformes [Moreschalchi et al, 2007[Moreschalchi et al, , 2008. Within ray-finned fish of the infraclass Teleostei, the chromosomal distribution of the telomeric DNA sequences has been studied in fish species belonging to 15 orders: Anguilliformes [Salvadori et al, 1995], Batrachoidoformes [Merlo et al, 2007], Characiformes [de Marco Ferro et al, 2003;, Cypriniformes [Meyne et al, 1990;Gornung et al, 1998;Sola et al, 2003a;Ocalewicz et al, 2004a;Gromicho et al, 2006;Schmid et al, 2006], Gasterosteiformes [Ocalewicz et al, 2011], Gymnotiformes [Milhomem et al, 2008;Silva et al, 2009;Felippe and Foresti, 2010;Scacchetti et al, 2011], Mugiliformes [Gornung et al, 2004;Rossi et al, 2005], Osmeriformes , Perciformes [Mandrioli et al, 2000;Sola et al, 2000Sola et al, , 2003bChew et al, 2002;Molina and Galetti, 2002;Mota-Velasco et al, 2010;Wang et al, 2010;Jacobina et al, 2011;Ocalewicz and Sapota, 2011;Jankun et al, 2013], Pleuronectiformes [Cross et al, 2006;Ocalewicz et al, 2008a], Salmoniformes [Reed and Phillips, 1995;Abuin et al, 1996;…”

Section: Telomere Length In Fishesmentioning

confidence: 99%

Telomeres in Fishes

Ocalewicz¹

2013

Cytogenet Genome Res

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In fishes, as in other vertebrate species, the DNA component of the telomeres consists of the tandemly repeated TTAGGG motif. The length of the telomeric arrays in fishes ranges from 2 to 25 kb and shortens with age in some of the species. To date, chromosomal distribution of the telomeric DNA sequences has been examined in approximately 80 fish species of which about 42% show additional telomeric hybridization signals far from the chromosomal termini. Based on the chromosomal location, such internally located telomeric repeats may be classified into 4 categories: (1) telomeric DNA sequences located at the pericentromeric regions, (2) interstitial telomeric sites observed between centromeres and the bona fide telomeres, (3) telomeric DNA sequences that scatter along the nucleolus organizer regions, and (4) telomeric DNA repeats interspersed with the entire chromosomes. Most of the pericentromeric and interstitial telomeric sequences in fish are possible relicts of chromosome fusion events. The origin of the telomeric sequences co- localizing with the major rDNA sequences or scattered along the whole chromosomes is not clear. Internally located telomeric repeats are considered as ‘hot spots' for recombination and thus may potentially increase the rates of chromosome breaks and rearrangements leading to the various chromosomal polymorphisms in fishes. FISH with telomeric probe applied to metaphase spreads of androgenetic specimens that hatched from eggs exposed to ionizing radiation before insemination enabled the detection of small radiation-induced fragments of maternal chromosomes. Remnants of the irradiated chromosomes were found to be ring chromosomes with the interstitial telomeric signals, telomerless rings, fragments with fused sister chromatids, and linear fragments with telomeres detected at both of their ends. The increasing availability of techniques enabling the study of fish telomeres and telomerase and the easy access to numerous fish species strongly confirm that these animals are promising models in research concerning the role of telomeres and telomerase in vertebrate aging, repair of ionizing radiation-induced DNA double strand breaks, and chromosomal rearrangements.

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Localization of the (TTAGGG)_n telomeric sequence in zebrafish chromosomes

Cited by 18 publications

References 13 publications

Molecular and cytogenetic analysis of the telomeric (TTAGGG) n repetitive sequences in the Nile tilapia, Oreochromis niloticus (Teleostei: Cichlidae)

Molecular and cytogenetic analysis of the telomeric (TTAGGG) n repetitive sequences in the Nile tilapia, Oreochromis niloticus (Teleostei: Cichlidae)

Karyotype and chromosome location of characteristic tandem repeats in the pufferfish <i>Tetraodon nigroviridis</i>

Telomeres in Fishes

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