Localization of Transcriptional Co-activator CBP in the Ameloblasts and the Other Enamel Organ-derived Cells of the Rat Incisor

Nishikawa, Sumio

doi:10.1177/002215540205001104

Cited by 6 publications

(6 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…No previous studies have examined the role of CREB phosphorylation in ameloblasts. However, CREB-binding protein has been identified in the nuclei of rat incisor ameloblasts 35 , and mRNA expression of the OASIS gene (which encodes a CREB/ATF family member) has been detected in mouse inner enamel epithelium during the cap and bell stages (E14.5–E18.5) 36 . The TRPM7 kinase domain has been reported to phosphorylate CREB peptide in breast cancer cells 24 .…”

Section: Discussionmentioning

confidence: 99%

The crucial role of the TRPM7 kinase domain in the early stage of amelogenesis

Ogata

Tsumuraya

Oka

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Transient receptor potential melastatin-7 (TRPM7) is a bi-functional protein containing a kinase domain fused to an ion channel. TRPM7 is highly expressed in ameloblasts during tooth development. Here we show that TRPM7 kinase-inactive knock-in mutant mice (TRPM7 KR mice) exhibited small enamel volume with opaque white-colored incisors. The TRPM7 channel function of ameloblast-lineage cells from TRPM7 KR mice was normal. Interestingly, phosphorylation of intracellular molecules including Smad1/5/9, p38 and cAMP response element binding protein (CREB) was inhibited in ameloblasts from TRPM7 KR mice at the pre-secretory stage. An immunoprecipitation assay showed that CREB was bound to TRPM7, suggesting that direct phosphorylation of CREB by TRPM7 was inhibited in ameloblast-lineage cells from TRPM7 KR mice. These results indicate that the function of the TRPM7 kinase domain plays an important role in ameloblast differentiation, independent of TRPM7 channel activity, via phosphorylation of CREB.

show abstract

Section: Discussionmentioning

confidence: 99%

The crucial role of the TRPM7 kinase domain in the early stage of amelogenesis

Ogata

Tsumuraya

Oka

et al. 2017

Sci Rep

View full text Add to dashboard Cite

show abstract

“…There is limited knowledge about the role of CREBBP during tooth development. CREBBP was shown to be localized in the mouse oral ectoderm at E12.5 [Partanen et al, 1999] and then further in the ameloblast layer [Nishikawa, 2002]. A mouse model for RTS displays a hypoplastic maxilla with a narrow palate [Oike et al, 1999].…”

Section: Discussionmentioning

confidence: 99%

Repairing goat tibia segmental bone defect using scaffold cultured with mesenchymal stem cells

Liu

Fan

et al. 2010

J Biomed Mater Res

View full text Add to dashboard Cite

In this study, we investigated cellular biocompatibility in vitro and segmental bone defect repairing efficacy in vivo of a previously reported fibre-reinforced scaffold, nano-hydroxyapatite/collagen/poly (L-lactic acid) (PLLA)/chitin fibres (nHACP/CF). First, attachment, proliferation, and differentiation of the goat bone mesenchymal stem cells (GBMSCs) cultured on the nHACP/CF scaffolds were evaluated in vitro. The results showed that cells attached to the scaffolds well, and there was no significant difference in cell proliferation between cells on the scaffolds and cells on the polystyrene culture plates that were used as a control. The results also showed that alkaline phosphatase (ALP)/DNA of the cells cultured on the scaffolds was significantly higher than that on the control. The in vivo study compared the bone defect repairing efficacy of nHACP/CF scaffolds with that of autograft bone. Thirty-two adult male goats with 25-mm defects in their tibias at the same anatomic site were divided into four groups. The first group was implanted with the nHACP/CF with GBMSCs. The second group was implanted with autograft bone. The third group was implanted with the nHACP/CF. Nothing was implanted in the fourth group. Bone growth was evaluated by radiography, histology, and biomechanics. The results showed that although the nHACP/CF had new bone formation, it could not repair the defect fully while nHACP/CF with GBMSCs cultured and autograft bone could repair the segmental bone defect by 8 weeks after surgery, suggesting that nHACP/CF is an appropriate scaffold for bone tissue engineering.

show abstract

“…Mutation in the transcriptional coactivator CREB‐binding protein‐encoding gene [ CREBBP ] are responsible for RTSTS1 134. However, while this gene is expressed in the early epithelium and ameloblasts of the developing tooth, little is known about its function during odontogenesis 135,136. Other rare conditions associated with the formation of talon cusps are temtamy preaxial brachydactyly syndrome [TBPS; #605282], which is due to mutation in chondroitin sulphate synthetase [ CHSY1 ] and has been linked with BMP signaling137 and Mohr syndrome [OFDII; %252100].…”

Section: Anomalies Of Tooth Size and Morphologymentioning

confidence: 99%

Diseases of the tooth: the genetic and molecular basis of inherited anomalies affecting the dentition

Cobourne

Sharpe

2012

WIREs Developmental Biology

View full text Add to dashboard Cite

In humans, inherited variation in the number, size, and shape of teeth within the dentitions are relatively common, while rarer defects of hard tissue formation, including amelogenesis and dentinogenesis imperfecta, and problems associated with tooth eruption are also seen. In many cases, these anomalies occur in isolation, but they can also present as a feature of numerous well-characterized developmental syndromes. Complex reiterative signaling between the epithelium and mesenchyme is a feature of normal tooth development in the embryo, occurring from early patterning through morphogenesis, hard tissue formation and during root development. Significant events also occur during postnatal development of the dentition, including hard tissue maturation and tooth eruption. In the last decade, advances in human and mouse genetics have meant that in many cases candidate genes have been identified for these anomalies. These genes have provided a useful platform for developmental biologists, allowing them to begin elucidating how these signals interact to generate a functional dentition and understand the mechanisms underlying many of the anomalies that are seen in human populations. In this article, we review current concepts relating to the developmental biology of tooth number, size, and shape, formation of the dental hard tissues and eruption of the tooth into the oral cavity. We will focus on the molecular mechanisms underlying these processes in both health and disease.

show abstract

Localization of Transcriptional Co-activator CBP in the Ameloblasts and the Other Enamel Organ-derived Cells of the Rat Incisor

Cited by 6 publications

References 31 publications

The crucial role of the TRPM7 kinase domain in the early stage of amelogenesis

The crucial role of the TRPM7 kinase domain in the early stage of amelogenesis

Repairing goat tibia segmental bone defect using scaffold cultured with mesenchymal stem cells

Diseases of the tooth: the genetic and molecular basis of inherited anomalies affecting the dentition

Contact Info

Product

Resources

About