Localization of type II collagen in the lingual mucosa of rats during the morphogenesis of circumvallate papillae

Abstract

Iwasaki, S., Aoyagi, H. and Yoshizawa, H. 2011. Localization of type II collagen in the lingual mucosa of rats during the morphogenesis of circumvallate papillae.-Acta Zoologica (Stockholm) 92: 67-74.Immunoreactivity specific for type II collagen was recognized first in the mesenchymal connective tissue just beneath the circumvallate papilla placode in fetuses on E13. At this stage, most of the lingual epithelium was pseudostratified epithelium composed of one or two layers of cuboidal cells. However, the epithelium of the circumvallate papilla placode was composed of several layers of cuboidal cells. Immunoreactivity specific for type II collagen was detected mainly on the lamina propria just beneath the lingual epithelium of the rudiment of the circumvallate papilla in fetuses on E15 and on E17, and slight immunostaining was detected on the lamina propria around the rudiment. In fetuses on E19, immunoreactivity specific for type II collagen was widely and densely distributed on the connective tissue around the developing circumvallate papillae and on the connective tissue that surrounded the lingual muscle. Immunoreactivity specific for type II collagen was sparsely distributed on the lamina propria of central bulge. After birth, morphogenesis of the circumvallate papillae advanced gradually with the increase in size of the tongue. Immunoreactivity specific for type II collagen was distinctively distributed in the lamina propria around circumvallate papilla, in the central bulge, and in the connective tissue that surrounded the lingual muscle.Shin-ichi Iwasaki,

Fluorescence Immunohistochemistry by Confocal Laser-Scanning Microscopy for Studies of Semi-Ultrathin Specimens of Epoxy Resin-Embedded Samples

“…To demonstrate the effectiveness of our method, in the present study we examined the immunofluorescence of immuno-stained keratins and collagens and the images obtained, in transmission mode, of the lingual mucosa of the filiform and circumvallate papillary areas of tongues of fetus and juvenile rats by LSM. By combining images, we were able to define clearly the histological locations of keratin 13 (K13) and keratin 14 (K14) in the lingual epithelium (Iwasaki et al, 2006a(Iwasaki et al, , 2011a and those of collagen II (CII) and collagen III (CIII) in the lingual connective tissue , 2011b. Furthermore, we demonstrated that our newly developed technique for localization of pairs of antigens should be useful for investigations of very small specimens, such as fetal tissues and organs .…”