Localization of type III procollagen and prolyl 4-hydroxylase mRNAs in fibrotic human liver.

Yamada, Hiroyuki; Aida, Takeo; Taguchi, Katsuji; Asano, Gorō

doi:10.1267/ahc.24.191

Cited by 2 publications

(1 citation statement)

References 16 publications

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“…Most of the previous reports using the in situ hybridization technique adopted radioactive probes for the detection of pro-collagen mRNA in tissues (Hurme et al, 1991;Solberg et al, 1991;Larjava et al, 1990;Milani et al, 1990;Prosser et al, 1989;Scharffetter et al, 1989;Hayashi et al, 1986), except for studies using digoxigenin-labeled DNA probes (Yamada et al, 1991;Aida et al, 1990). We need much higher resolution for the study of diffusetype carcinoma of the stomach, in which isolated carcinoma cells and stromal cells are closely intermingled.…”

Section: Immunoelectron Microscopymentioning

confidence: 99%

Identification of type I collagen-producing cells in human gastrointestinal carcinomas by non-radioactive in situ hybridization and immunoelectron microscopy.

Ohtani

Kuroiwa²,

Obinata³

et al. 1992

J Histochem Cytochem.

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Invasive growth of cancer cells induces desmoplastic reaction as one of the host reactions. It has been a matter of controversy whether stromal collagen is produced by cancer cells or stromal fibroblasts. In the present study, we investigated the cellular origin of Type I collagen in human gastrointestinal carcinomas by in situ hybridization and immunoelectron microscopy. In situ hybridization technique with digoxigenin-labeled RNA probes revealed that spindle-shaped fibroblasts in the stromal area were abundantly positive for transcripts of pro alpha 1(I) collagen in intestinal-type adenocarcinoma. Gland-forming carcinoma cells were negative. In diffuse-type carcinoma of the stomach, spindle-shaped or stellate fibroblasts were positive, whereas dissociated, oval carcinoma cells were negative. A precise determination of cell type was done by immunoelectron microscopy. Intracellular immunoreactivity for Type I collagen was observed in rough endoplasmic reticulum of fibroblasts (including myofibroblasts) in the stromal area. No definite reactivity was obtained in cancer cells by either in situ hybridization or immunoelectron microscopy. Our results indicated that stromal Type I collagen is produced by stromal fibroblasts, which are activated by cancer invasion.

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Section: Immunoelectron Microscopymentioning

confidence: 99%