Localization of vascular endothelial growth factor in synovial membrane mast cells: examination with ”multi-labelling subtraction immunostaining"

Yamada, Takahiro; Sawatsubashi, Motohiro; Yakushiji, Hiroyuki; Itoh, Yumi; Edakuni, Genichiro; Mori, Michito; Robert, L. Price; Miyazaki, Kohji

doi:10.1007/s004280050290

Cited by 33 publications

(22 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Angiogenesis associated with physiological events, such as embryonic development, ovulation and wound healing, has been found to be accompanied by tissue accumulation of MC [15,16]. In addition, an increased number of MC have been reported in angiogenesis associated with chronic inflammatory diseases (rheumatoid arthritis, nasal polyps) [17,18], vascular neoplasms (haemangiomas) [19], as well as solid and haematopoietic tumors (gastric cancer, neurofibroma, lung carcinomas, laryngeal squamous cell carcinoma, cutaneous melanoma, B-cell non-Hodgkin's lymphomas, multiple myeloma, myelodysplastic syndromes, chronic lymphocytic leukemia) [20][21][22][23][24][25][26][27][28][29][30][31]. In tumor samples, MC density correlates with angiogenesis and poor tumor outcome [20,32,33].…”

Section: Mast Cells and Angiogenesismentioning

confidence: 97%

Involvement of Mast Cells in Angiogenesis and Chronic Inflammation

Crivellato

Ribatti

2005

CDTIA

View full text Add to dashboard Cite

Mast cells (MC) are granulated secretory cells that have long been recognized as a rich source of biologically highly active mediators such as biogenic amines, prostaglandins, leukotrienes, proteases, cytokines and chemokines. Most of their biological functions however has been rather elusive. There are now emerging data assigning these cells a relevant role in orchestrating angiogenesis, both in normal and pathological conditions. MC indeed synthesize and release a large array of proangiogenic factors upon different stimulation pathways. In addition, MC have been recognized as key cells in mediating host innate and adaptive immune responses. This review summarizes the most recent acquisitions concerning MC involvement in angiogenic processes and chronic inflammatory reactions.

show abstract

Section: Mast Cells and Angiogenesismentioning

confidence: 97%

Involvement of Mast Cells in Angiogenesis and Chronic Inflammation

Crivellato

Ribatti

2005

CDTIA

View full text Add to dashboard Cite

show abstract

“…Double-labelling subtraction immunostaining was carried out as previously reported (Yamada et al, 1998). Briefly, after routine immunostaining for COX-2, the slide was treated with 3-amino-9 ethylcarbazole (Sigma, St. Louis, MO, USA).…”

Section: Double-labelling Subtraction Immunostainingmentioning

confidence: 99%

Expression of cyclooxygenase-2 (COX-2) in tumour and stroma compartments in cervical cancer: clinical implications

et al. 2002

View full text Add to dashboard Cite

This study aims at investigating the relationship between cyclooxygenase-2 expression in tumour vs stroma inflammatory compartment and its possible clinical role. The study included 99 stage IB-IV cervical cancer patients: immunostaining of tumour tissue sections was performed with rabbit antiserum against cyclooxygenase-2. CD3, CD4, CD8, CD25, Mast Cell Tryptase monoclonal antibodies were used to characterise stroma inflammatory cells in nine cervical tumours. An inverse relation was found between cyclooxygenase-2 levels (cyclooxygenase-2 IDV) of tumour vs stroma compartment (r=70.44, P50.0001). The percentage of cases showing high tumour/stromal cyclooxygenase-2 IDV ratio was significantly higher in patients who did not respond to treatment (93.3%) with respect to patients with partial (60.5%), and complete (43.7%) response (P= 0.009). Cases with a high tumour/stroma cyclooxygenase-2 IDV ratio had a shorter overall survival rate than cases with a low tumour/stroma cyclooxygenase-2 IDV (P50.0001). In the multivariate analysis advanced stage and the status of tumour/stroma cyclooxygenase-2 IDV ratio retained an independent negative prognostic role. The proportion of CD3 + , CD4 + , and CD25 + cells was significantly lower in tumours with high tumour/stroma cyclooxygenase-2 IDV ratio, while a higher percentage of mast cells was detected in tumours showing high tumour/stroma cyclooxygenase-2 IDV ratio. Our study showed the usefulness of assessing cyclooxygenase-2 status both in tumour and stroma compartment in order to identify cervical cancer patients endowed with a very poor chance of response to neoadjuvant therapy and unfavourable prognosis.

show abstract

“…lactogenic) condition (Michels, 1963). Although mast cells and mast cell factors are strongly associated with angiogenesis during wound healing and cancer (Kessler et al, 1976; Eady et al, 1979; Azizkhan et al, 1980; Folkman, 1985; Wilson, 1985; Starkey et al, 1988; Takeda et al, 1989; Meininger and Zetter, 1992; Gordon and Galli, 1994a,b; Jakobsson, 1994; Qu et al, 1995; Norrby, 1997; Blair et al, 1997; Yamada et al, 1998; Soucek et al, 2007), the role of these cells in physiological angiogenesis of the mammary gland during the estrous cycle, lactogenesis, and involution has not been described. The purpose of this study was to compare the changes in mast cell abundance with the development and regression of the lobular capillary plexus that occurs during the expansive growth of lactogenesis, the regressive remodeling of postlactational involution, and less extensive expansive and regressive remodeling that take place within the estrous cycle.…”

Section: Introductionmentioning

confidence: 99%

Alterations in mast cell frequency and relationship to angiogenesis in the rat mammary gland during windows of physiologic tissue remodeling

Ramirez

Lee

Schedin

et al. 2012

Developmental Dynamics

View full text Add to dashboard Cite

Background The mammary epithelium undergoes proliferation and regression accompanied by remodeling of the fibrocellular and vascular stroma. Mast cells are abundant in these compartments and have been implicated in remodeling during wound healing and cancer progression. The purpose of this study was to test the hypothesis that mast cell abundance correlates with physiologic mammary tissue remodeling during estrous cycling, lactogenesis (pregnancy and lactation) and involution. Results Mast cell and capillary frequency were quantified in the stroma surrounding ducts and lobules from mammary glands of rats. During estrous cycling, periductal mast cell numbers were unchanged, but lobule-associated mast cells significantly increased in the regressive phase of diestrus II. During lactogenesis, lobular stroma mast cells peaked early in pregnancy, at D2, followed by a significant decrease throughout lactation. Involution was associated with a rapid return in mast cell numbers, similar to diestrus II. Lobular vascularization peaked during the state of metestrus, when limited secretory differentiation occurs. Lobular angiogenesis peaked at D7 of pregnancy, regressed, and then returned to high levels during lactation and early involution, when secretory differentiation is high. Conclusions These results suggest mast cells are predominantly associated with regressive lobular remodeling during cycling and involution, whereas angiogenesis is predominantly associated with secretory differentiation.

show abstract

Localization of vascular endothelial growth factor in synovial membrane mast cells: examination with ”multi-labelling subtraction immunostaining"

Cited by 33 publications

References 14 publications

Involvement of Mast Cells in Angiogenesis and Chronic Inflammation

Involvement of Mast Cells in Angiogenesis and Chronic Inflammation

Expression of cyclooxygenase-2 (COX-2) in tumour and stroma compartments in cervical cancer: clinical implications

Alterations in mast cell frequency and relationship to angiogenesis in the rat mammary gland during windows of physiologic tissue remodeling

Contact Info

Product

Resources

About