In [J. Buckner, M. Dugas, Co-local subgroups of abelian groups, in: Abelian Groups, Rings, Modules, and Homological Algebra, in: Lect. Notes Pure and Applied Math., vol. 249, Taylor and Francis/CRC Press, pp. 25-33] the notion of a colocal subgroup of an abelian group was introduced. A subgroup K of A is called co-local if the natural map Hom(A, A) → Hom(A, A/K ) is an isomorphism. At the center of attention in [J. Buckner, M. Dugas, Co-local subgroups of abelian groups, in: Abelian Groups, Rings, Modules, and Homological Algebra, in: Lect. Notes Pure and Applied Math., vol. 249, Taylor and Francis/CRC Press, pp. 25-33] were co-local subgroups of torsion-free abelian groups. In the present paper we shift our attention to co-local subgroups K of mixed, non-splitting abelian groups A with torsion subgroup t (A). We will show that any co-local subgroup K is a pure, cotorsion-free subgroup and if D/t (A) is the divisible part of A/t (A) = D/t (A)⊕ H/t (A), then K ∩ D = 0, and one may assume that K ⊆ H . We will construct examples to show that K need not be a co-local subgroup of H . Moreover, we will investigate connections between co-local subgroups of A and A/t (A).