Localized Bone Marrow Transplantation Leads to Skin Allograft Acceptance in Nonmyeloablated Recipients: Comparison of Intra‐Bone Marrow and Isolated Limb Perfusion

Abstract

2002;20:86-93

Donor–origin cell engraftment after intraosseous or intravenous bone marrow transplantation in a rat model

“…Intraosseous BMT may therefore provide an acceptable alternative in restrictive circumstances, as when the number of cells to be transplanted is limited; for example, in the transplantation of cord blood cells. 6 There are additional circumstances in which this innovative method of direct intraosseous BMT could be applied: (1) when transplanting a highly selected cell population; 9,10 (2) when transplanting a selected cell population modified by gene transfer; (3) in organ transplants as supportive therapy for inducing donor-specific immune unresponsiveness; 8,11 and (4) as a more effective method of co-transplanting hematopoietic and MSCs 12 or in conjunction with donor lymphocyte infusion. 7 In summary, our study supports the view that short-term selective T-cell depletion under ab-TCR/CsA protocol combined with intraosseous BMT (70 Â 10 6 ) is more efficient method for donor-cell engraftment when compared to i.v.…”

“…Hematopoietic recovery was rapid and T-cell function was restored. [4][5][6][7][8][9][10][11][12] Recently this promising new strategy of intraosseous stem cell engraftment was applied in nonhuman primates under conditioning with radiation. 13 We previously reported the effect of different routes and dosages of BMC transplantation on chimerism induction in the peripheral blood.…”

Transplanted Hematopoietic Cells Seed in Clusters in Recipient Bone Marrow In Vivo

“…For this reason, measurements performed 24 hours posttransplant were easier. In addition, within 3-5 hours after injection, there were no detectable levels of labeled donor cells in the peripheral blood of the recipients [24]. The intravascular space is usually invisible to in vivo fluorescence imaging, and the observations represent cells in the marrow space (extravascular).…”

Cardiac Allograft Acceptance after Localized Bone Marrow Transplantation by Isolated Limb Perfusion in Nonmyeloablated Recipients

“…In fact, injection of the cells into a smaller hematopoietic space creates a local megadose effect, shown to improve the efficiency of T-cell-depleted BMC (TCD-BMC) and stem cell engraftment across antigen barriers without myeloablative conditioning [17,18]. Experimentally, there is evidence that infusion of cells into the BM and into the IL is associated with a lower incidence of GVHD and superior engraftment of TCD-BMCs [13,14]. Assessment of the early fate of donor cells after intra-BM injection revealed systemic dissipation of the cells, suggesting that this procedure was very similar to infusion into a peripheral vein [14].…”