Localized surface plasmon resonance (LSPR) study of DNA hybridization at single nanoparticle transducers

“…LSPR is a label-free detection scheme that is based on measuring the shift in extinction spectra of plasmonic nanoparticles occurring when the local dielectric constant around the particles changes, such as from DNA binding to probes immobilized on the nanoparticle surface. 28, 29 …”

Section: Introductionmentioning

confidence: 99%

Rapid real-time recirculating PCR using localized surface plasmon resonance (LSPR) and piezo-electric pumping

2017

View full text Add to dashboard Cite

Rapid detection and characterization of pathogens in patients with bloodstream infections (BSIs) is a persistent problem for modern medicine, as current techniques are slow or provide incomplete diagnostic information. Real-time polymerase chain reaction (qPCR) allows specific detection of a wide range of targets and quantification of pathogenic burden to aid in treatment planning. However, new technological advances are required for a rapid and multiplex implementation of qPCR in clinical applicatons. In this paper, feasibility of a novel microfluidic platform for qPCR is presented integrating highly sensitive, label-free localized surface plasmon resonance (LSPR) imaging of DNA hybridization in a recirculating chip design for real-time analysis. Single target and multiplex detection of DNA target amplification are demonstrated, with a limit of detection of 5fg/uL of E.coli DNA for single target PCR, correlating to approximately 300 bacteria per mL. The results of this study demonstrate potential of this platform for simultaneous real-time detection of multiple target genes whithin 15 minutes that could provide live saving benefits in patients with BSIs.

show abstract

“…These factors can be adjusted by the chemical synthesis. Different applications as biochip-or cell-label [4,5], as optical antenna for manipulation of biomolecules [6,7], or as sensor transducer [8] are based on this potential.…”

Section: Introductionmentioning

confidence: 99%

“…The application of gold spheres (LSPR by 520 nm) in bioanalytics is widely spread [8][9][10]. These particles are chemically relative stable, their synthesis and biofunctionalization is wellestablished, and they are well biocompatible.…”

Section: Introductionmentioning

confidence: 99%

High‐Throughput Synthesis of Uniform Silver Seed Particles by a Continuous Microfluidic Synthesis Platform

et al. 2015

View full text Add to dashboard Cite

Controlled silver particle geometries require at least a synthesis in two steps which strongly differ in their reaction kinetics. For the first step, the very fast seed formation, chaotic advection-based micromixers are tested in combination with a batch reactor for the growth step. Nanoparticles with narrow size distribution and excellent shape uniformity can be prepared in large batches. To achieve a highly reproducible and homogeneous particle solution, a microfluidic system containing three different micromixers for optimal mixing of the chemical precursors is established, allowing stringent control of every synthesis step. The produced silver particles can be used as seeds for forming anisotropic particles. Their further potential is demonstrated by preparation of anisotropic silver triangles. The thus generated seed particles are better suited for growing to triangles than those from conventional batch synthesis.

show abstract

Localized surface plasmon resonance (LSPR) study of DNA hybridization at single nanoparticle transducers

Cited by 49 publications

References 32 publications

Detection, Counting, and Imaging of Single Nanoparticles

Detection, Counting, and Imaging of Single Nanoparticles

Rapid real-time recirculating PCR using localized surface plasmon resonance (LSPR) and piezo-electric pumping

High‐Throughput Synthesis of Uniform Silver Seed Particles by a Continuous Microfluidic Synthesis Platform

Contact Info

Product

Resources

About