Locally Injected Autologous Platelet-Rich Plasma Improves Cutaneous Wound Healing in Cats

Abstract

Cutaneous defects in cats are commonly encountered in clinical practice, and healing can be accomplished by first or second intention. Platelet-rich plasma (PRP) is characterized by a plasma concentration containing a large number of platelets in a small volume of plasma. The objective of the present study was to record the efficacy of PRP infiltration in open wounds in laboratory cats. Six wounds were created in the dorsal midline of eight laboratory cats, with the wounds of one side designated as the PRP group and the wounds of the other side as the control group. Wound healing was evaluated by daily clinical examination, planimetry, laser Doppler flowmetry, and histologic examination on days 0, 7, 14, and 25, and by measurement of metalloproteinases (MMPs)-2 and -9 and tissue inhibitor metalloproteinase (TIMP)-1 on days 0, 14, and 25. Based on the results of the present study, the mean time for full coverage with granulation tissue was shorter in the PRP group, the mean contraction and total wound healing percentage were increased compared to the control group, and finally, the perfusion measured with laser Doppler flowmetry was higher in the PRP group during all examination days. In conclusion, this is the first study focusing on the topical application of PRP in the treatment of open wounds in laboratory cats, and our results are encouraging—showing a more rapid healing in the PRP group.

Evaluation of Platelet-Rich Plasma by means of PRGF®-Endoret® protocol in leukemia cats: PDGF-BB and TGF-ß1 valuation

“…While System 1 reduced PLT concentrations by 3%, System 2 increased PLT concentrations by 187% ( 28 ). To evaluate the effect of local autologous PRP on healing by secondary intention of skin disorders in cats, a recent study by Angelou et al ( 32 ) included 11 mL of WB in a vacuum tube containing separating gel and anticoagulant (MACD7) and centrifuged at 1,500 rpm for 10 min. PLT counts in the obtained PRP product increased from 2 to 8.2 times compared to WB in this latter study ( 32 ).…”

“…To evaluate the effect of local autologous PRP on healing by secondary intention of skin disorders in cats, a recent study by Angelou et al ( 32 ) included 11 mL of WB in a vacuum tube containing separating gel and anticoagulant (MACD7) and centrifuged at 1,500 rpm for 10 min. PLT counts in the obtained PRP product increased from 2 to 8.2 times compared to WB in this latter study ( 32 ). Even though we cannot specify the exact origin of these differences, factors such as WB volume samples, gravitational force, and centrifugation times in the different protocols used to obtain PRP, should be taken into account since the basal PLT number in the WB fraction were similar.…”

“…The PRGF ® -Endoret ® protocol for obtaining GFs is widely documented in different species and has been successfully applied in different clinical areas ( 9 – 11 ), such as regenerative medicine for wound healing, ophthalmology, dentistry, osteoarthritis, tendinopathies, or aesthetic medicine in humans ( 12 – 19 ); traumatology and ophthalmology in dogs ( 20 – 22 ) and horses ( 23 ); and wound healing ( 24 , 25 ) or the treatment of complete cartilage defects in rabbits ( 26 ). Although different protocols for obtaining PRP in cats have been described ( 27 – 32 ), the use of PRGF ® -Endoret ® technology in this species has only been reported in a previous study carried out by the same researchers. Due to the cellular characteristics of the autologous blood product obtained following centrifugation, it was classified as a P-PRP product ( 33 ).…”

Evaluation of the Effectiveness of Medical-Grade Honey and Hypericum Perforatum Ointment on Second-Intention Healing of Full-Thickness Skin Wounds in Cats

“…A better insight into the healing mechanism of felines and local wound care products is warranted to improve healing [ 3 ]. Studies have been performed comparing many wound care products in second-intention healing in canines [ 4 , 5 , 6 , 7 ] and very few in feline patients [ 8 , 9 , 10 ].…”

Effective treatment of a severe neck wound in a greylag goose (Anser anser) using autologous thrombocyte‐rich plasma and xenogeneic fibrin mesh

“…Platelet-rich plasma and platelet lysate have demonstrated the potential to promote wound healing. [1][2][3][4][5][6][7] Comprehensive database searches were conducted using freely accessible databases such as Google Scholar (https:// scholar.google.com), PubMed (https://pubmed.ncbi.nlm.nih. gov) and SciELO (https://www.scielo.org), spanning from 1950 to 2023.…”