The optical frequency domain reflectometry (OFDR) demodulation technology based on DBR laser and spectral splicing was proposed in our previously studied. Spectral splicing solves the problem of laser mode hopping that is not allowed in the original OFDR demodulation technology. However the detailed analysis of the influence of spectral splicing on the spatial resolution of OFDR didn't been provided. Based on previous research, the influence of spectral splicing on the spatial resolution of OFDR was analyzed in detail. The effects of single-segment wavelength range, number of spectral splicing segments, and splicing error on spatial resolution of OFDR were simulated. The results indicate that the spatial resolution of OFDR after spectral splicing is determined by the single-segment wavelength range, and it has the same spatial resolution as demodulating only one section of the spectrum. Meanwhile, the spatial resolution is independent of the number of spectral splicing segments and splicing errors (within 10 pm). Therefore, in order to improve the spatial resolution of OFDR, the single-segment wavelength range should be expanded as much as possible. Finally, experimental testing was conducted to verify the effectiveness. 35 spectral segments were achieved using a DBR laser, with each segment having a wavelength range of 1.2nm. By identifying the peak width of the fiber optic connector, it was verified that the spatial resolution of multi-segment spectral splicing and single-segment spectral is the same. However, experiments have also shown that spectral splicing weakens the measurement noise of the system, which is one of its advantages.