Location matters: Offset in tissue-engineered vascular graft implantation location affects wall shear stress in porcine models

Patient-specific tissue engineered vascular graft for aortic arch reconstruction

“…The MRI acquisition involved a 4-dimensional (4D) time-resolved magnetic resonance angiography with keyhole sampling scheme over the cardiothoracic region using a sagittal view, with acquisition parameters stated in previous work. 17 Magnetic resonance angiography image segmentation, stereolithography file export methods, CFD simulation and optimization ( Figure 2 ), and graft design methods using CAD have been described in previous work. 17

Figure 2 Three-dimensional ( 3D ) tissue-engineered vascular graft optimization using computer-aided design and computational fluid dynamics.

…”

“… 17 Magnetic resonance angiography image segmentation, stereolithography file export methods, CFD simulation and optimization ( Figure 2 ), and graft design methods using CAD have been described in previous work. 17

Figure 2 Three-dimensional ( 3D ) tissue-engineered vascular graft optimization using computer-aided design and computational fluid dynamics. A, The 3D model of the surrounding heart and vasculature anatomy overlaid with the new graft design to test anatomical fit.

…”

“…The detailed preparation and sterilization of the grafts are described in detail in previous work. 17 …”

“…The same histological, mechanical, and statistical analysis methods were used from previous work; however, CD 68 (Abcam, ab955) and CD 206 (Novus Biologicals, NBP1-90020) were added for immunofluorescence testing. 16 , 17 …”