Location of Calcium and Phosphorus in Ashed Spores of <i>Bacillus megaterium</i>, Determined by Electron Probe X‐Ray Microanalysis

Nishihara, Tsutomu; Kondo, Masaomi; Nonaka, Tokio; Higashi, Youichirou

doi:10.1111/j.1348-0421.1982.tb00166.x

Cited by 5 publications

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References 16 publications

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“…Calcium distribution studies using high-resolution electron probe X-ray microanalysis (15,23,24,35) revealed that only a fraction of the total spore calcium was detected in the integuments. The bulk portion (> 98-99%) of the spore calcium was found in the core region.…”

Section: Restoration By Calcium Of L-alanine-induced Germinabilities mentioning

confidence: 99%

“…Only a small fraction of the total spore calcium is believed to be associated with the spore integuments, i.e., coat and cortex (23,24). The function of this integumentassociated calcium is not known.…”

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confidence: 99%

“…It is known from electron probe X-ray microanalysis that more than 98-99% of the spore calcium is located in the spore core region (15,23,24,35). Only a small fraction of the total spore calcium is believed to be associated with the spore integuments, i.e., coat and cortex (23,24).…”

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confidence: 99%

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Involvement of Calcium in Germination of Coat‐Modified Spores of Bacillus cereus T

Shibata

Miyoshi

Osato

et al. 1992

Microbiology and Immunology

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The effect of calcium on germination of coat-modified Bacillus cereus T spores was investigated. Coat-modified spores produced either by chemical extraction (SDS-DTT-treated spores) or by mutagenesis (10LD mutant spores) were unable to germinate in response to inosine. While SDS-DTT-treated spores could germinate slowly in the presence of L-alanine, l OLD mutant spores could not germinate at all. The lost or reduced germinability of coat-modified spores was restored when exogenous Ca2+ was supplemented to the germination media. The calcium requirement of coat-modified spores for germination was fairly specific. The simultaneous presence of germinant with Ca2+ was also required for germination of coat-modified spores. The optimal recovery of germinability was obsei ved in the presence of 1.0 mm of calcium acetate. The calcium requirement itself was remarkably diminished under the condition in which L-alanine and a certain purine nucleoside analog, adenosine or inosine, coexisted. The lost or diminished germinability observed in SDS-DTT-treated spores or l OLD mutant spores may be attributed to the loss of calcium associated with the spore integuments.

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Section: Restoration By Calcium Of L-alanine-induced Germinabilities mentioning

confidence: 99%

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confidence: 99%

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Involvement of Calcium in Germination of Coat‐Modified Spores of Bacillus cereus T

Shibata

Miyoshi

Osato

et al. 1992

Microbiology and Immunology

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“…On the other hand, it has been shown that spores of B. megaterium can be divided into two groups from the morphological and chemical features of the coat and other properties (12). B. megaterium ATCC 12872, which had been designated as QM B1551 (8) and was examined in our previous study (6), is a representative strain of one group having a thick coat with high phosphate content, while ATCC 19213, which was used in this study, is a representative strain of the other group having a thin coat with low phosphate content (10,12).…”

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confidence: 99%

“…B. megaterium ATCC 12872, which had been designated as QM B1551 (8) and was examined in our previous study (6), is a representative strain of one group having a thick coat with high phosphate content, while ATCC 19213, which was used in this study, is a representative strain of the other group having a thin coat with low phosphate content (10,12).…”

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17K‐Spore Coat Protein Antigen in Sporulating Cells of Bacillus megaterium ATCC 19213

El‐Belbasi

Imagawa

Nishihara

et al. 1987

Microbiology and Immunology

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Microincineration and elemental X-ray microanalysis of single Bacillus cereus T spores

Scherrer¹,

Shull²

1987

Can. J. Microbiol.

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Single whole spores of bacillus cereus T were analyzed by scanning electron microscopy and electron microprobe X-ray microanalysis before and after high-temperature (600 degrees C) ashing in air. High-temperature ashing consisted of a centripetal oxidation of the spore surface combined with pyrolysis of the spore's interior. Ashing of single spores produced a compact central ash particle, mimicking the much larger unashed spore body in outline but containing craterlike microregions, and a peripheral thin ash film. Ashing mostly eliminated the spore's organic matrix; however, ash residues still gave residual carbon-characteristic X-ray counts. Ashing of single spores produced a two-, five-, and six-fold increase of potassium, magnesium, and calcium X-ray intensities, respectively. Iron, although low in actual counts, became detectable after ashing. Phosphorus characteristic X-rays were decreased by 41% after ashing, while volatilization lowered sodium and manganese X-ray intensities by over 80%. High-temperature ashing enhanced element-characteristic X-ray intensities of the non-volatilizable mineral(ized) elements of spores by compacting them into ash residues, more so than by simply abolishing their organic matrix. Microincineration appears a generally useful preconcentration technique for elemental detection and localization in X-ray microanalysis.

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Location of Calcium and Phosphorus in Ashed Spores of Bacillus megaterium, Determined by Electron Probe X‐Ray Microanalysis

Cited by 5 publications

References 16 publications

Involvement of Calcium in Germination of Coat‐Modified Spores of Bacillus cereus T

Involvement of Calcium in Germination of Coat‐Modified Spores of Bacillus cereus T

17K‐Spore Coat Protein Antigen in Sporulating Cells of Bacillus megaterium ATCC 19213

Microincineration and elemental X-ray microanalysis of single Bacillus cereus T spores

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