1986
DOI: 10.1128/jb.167.2.660-665.1986
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Location of the antigenic determinants of conjugative F-like pili

Abstract: The amino terminus of the pilin protein constitutes the major epitope of F-like conjugative pili studied to date (F, ColB2, R1-19, R100-1, and pED208). Anti-pED208 pilus antibodies were passed through a CNBr-Sepharose affinity column linked to bovine serum albumin which was conjugated to a synthetic peptide, AcP(1-12), containing the major epitope at the amino terminus of pED208 pilin. This allowed the separation of two classes of antibodies; one was specific for the amino terminus and bound to the column, whi… Show more

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Cited by 22 publications
(9 citation statements)
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“…We observed occasional immunostaining vesicles in F' lac cultures, but much less frequently and generally not as heavily stained (Fig. SA, inset); similar vesicles were present in preparations examined by Worobec et al (29). The staining appears not to reflect adventitious association of F pilin with membrane debris, since much apparently vesicular material did not stain (Fig.…”
Section: Resultsmentioning
confidence: 65%
See 1 more Smart Citation
“…We observed occasional immunostaining vesicles in F' lac cultures, but much less frequently and generally not as heavily stained (Fig. SA, inset); similar vesicles were present in preparations examined by Worobec et al (29). The staining appears not to reflect adventitious association of F pilin with membrane debris, since much apparently vesicular material did not stain (Fig.…”
Section: Resultsmentioning
confidence: 65%
“…The dominant antigenic determinant of F pilin, and of other conjugative pilins as well, is at the extreme amino terminus of the molecule (6,8,29,30). F pili failed to react with monoclonal antibodies to amino-terminal epitopes of F pilin, suggesting that the amino acid terminus is masked in the filament (9).…”
mentioning
confidence: 99%
“…for 24 h with 20 pLg of lipopolysaccharide (LPS; E. coli serotype 0.26:B6; Sigma Chemical Co., St. Louis, Mo.) per ml before fusion with myeloma MOPC 315.43 cells as described previously (13).…”
Section: Methodsmentioning
confidence: 99%
“…Antiserum was absorbed with E. coli J53-1 cells (two plates of heavily inoculated cells suspended in 0.1 M phosphate-buffered saline) sonicated for five 30-s cycles. Immunogold labeling experiments were carried out as previously described (15) before electron microscopic examination with a Phillips 300 transmission electron microscope.…”
mentioning
confidence: 99%