Location of the structural gene of pseudorabies virus glycoprotein complex gII

Mettenleiter, Thomas C.; Lukàcs, Noèmi; Thiel, Heinz-Jürgen; Schreurs, C; Rziha, Hanns-Joachim

doi:10.1016/0042-6822(86)90372-7

Cited by 42 publications

(34 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One glycoprotein, gX, is secreted into the culture medium (Rea et al, 1985). Up to now, six glycoprotein genes have been mapped on the viral genome and sequenced (Mettenleiter et al, 1985a(Mettenleiter et al, , 1986Petrovskis et al, 1986a, b;Rea et aL, 1985;Robbins et al, 1986Robbins et al, , 1987Wathen & Wathen, 1984). The genes for gI, gp63, gp50 and gX reside in Us whereas the genes for gII and gIII are localized in UL (Mettenleiter et al, 1986;Robbins et al, 1986Robbins et al, , 1987.…”

Section: Discussionmentioning

confidence: 99%

“…The glycoprotein gII, a homologue of the herpes simplex virus type 1 (HSV-1) glycoprotein gB, is a major constituent of the viral envelope (Hampl et al, 1984;Luk~tcs et al, 1985) and is represented by a complex of three related glycoproteins that are derived from a common precursor protein (Mettenleiter et al, 1986). Two of these glycoproteins are considered to arise by proteolytic cleavage of the larger precursor polypeptide and are linked by disulphide bonds (Hampl et al, 1984;LukS.cs et al, 1985).…”

Section: Discussionmentioning

confidence: 99%

“…Two of these glycoproteins are considered to arise by proteolytic cleavage of the larger precursor polypeptide and are linked by disulphide bonds (Hampl et al, 1984;LukS.cs et al, 1985). The structural gene for the glI complex has been mapped to Sail subfragments 1A and 1G of BamHI fragment 1 (map units 0.105 to 0-130) (Mettenleiter et al, 1986), and the 3' end of the coding region has been located in Sail fragment 1G (Robbins et aL, 1987).…”

Section: Discussionmentioning

confidence: 99%

“…In order to map the glI gene more precisely Sail subfragments ofBarnHI 1 of PRV strain Ka (Kaplan & Vatter, 1959) have been cloned into pBR325 and designated 1A to IH according to their size (Mettenleiter et al, 1986) (Fig. 1) (Mettenleiter et al, 1986 SalI subfragment 1G did not appear as one distinct band but seemed to consist of two submolar restriction fragments. Both fragments were isolated and cloned.…”

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Pseudorabies Virus Displays Variable Numbers of a Repeat Unit Adjacent to the 3' End of the Glycoprotein gII Gene

Simon¹,

Mettenleiter²,

Rziha³

1989

Journal of General Virology

View full text Add to dashboard Cite

SUMMARYThe coding region of the glycoprotein complex glI of pseudorabies virus (PRV) is located in the unique long part of the genome on Sail subfragments 1A and 1G of BamHI fragment 1 (map units 0.105 to 0.130). Fragment 1G which includes the 3' end of the gII gene displays a size heterogeneity among different PRV strains and also within plaque isolates of a given strain. To reveal the cause of this heterogeneity and whether it might affect the gII-coding region we sequenced different l G fragments of the PRV strains Ka, Phylaxia and Dessau, and determined the 3' end of the glI mRNA by SI analysis. These data show that the size heterogeneity is caused by the presence of a variable number of tandemly repeated DNA sequence downstream but adjacent to the coding region of the glycoprotein gII gene. The 3' end of the gII mRNA was mapped about 24 bp upstream of the first repeat unit. A 15 bp sequence 5' GGGACGGAGGGGAGA 3' is repeated from three to over 50 times in different 1G fragments. It is the only repeat unit present in strain Ka, whereas the Phylaxia and Dessau strains show additional modifications.Pseudorabies virus (PRV) or suid herpesvirus 1 (Roizman et aL, 1981) contains a linear, double-stranded DNA genome of approximately 150 kbp. It is composed of two sets of unique sequences, UL and Us. Us is bounded by inverted repeats and is found in two orientations relative to UL. After PRV infection several glycoproteins are synthesized and incorporated into the viral envelope and the plasma membrane of the infected cell. One glycoprotein, gX, is secreted into the culture medium (Rea et al

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Pseudorabies Virus Displays Variable Numbers of a Repeat Unit Adjacent to the 3' End of the Glycoprotein gII Gene

Simon¹,

Mettenleiter²,

Rziha³

1989

Journal of General Virology

View full text Add to dashboard Cite

show abstract

“…During recent years much effort has been made to investigate the glycoproteins of PRV regarded as major targets for the host's immune response. To date seven glycoprotein genes have been mapped on the viral genome and sequenced (Hampl et al, 1984;Luk/tcs et al, 1985;Mettenleiter et al, 1985aMettenleiter et al, , 1986Petrovskis et al, 1986aPetrovskis et al, , b, 1988Rea et al, 1985;Robbins et al, 1986aRobbins et al, , 1987Wathen & Wathen, 1984. Four of these glycoproteins, gI, gp63, gill and gX, are not required for virus growth in cell culture (BenPorat et al, 1986;Mettenleiter et al, 1985a;Petrovskis et al, 1986c;Rea et al, 1985;Robbins et al, 1986b;Wathen & Wathen, 1986).…”

Section: Introductionmentioning

confidence: 99%