Surface water on the mainly dry, upland interfluves of the Upper Coastal Plain of South Carolina occurs currently as a sporadic distribution of shallow ponds held within Carolina bays and other small, isolated basins. At seven bays on the US. Department of Energy's Savannah River Site on the Upper Coastal Plain of South Carolina, we investigated Holocene changes in bay morphology, ecology, and prehistoric human activity. At Flamingo Bay, we employed archaeological survey and testing, shovel and auger testing, sediment analysis, and ground-penetrating radar to document stratigraphy and chronology of the sand rim on the eastern side of the bay. Artifact assemblage indicate changes in intensity of human use of the bay. Radiocarbon dates from a sediment core establish time scales for depositional processes at the center of the basin. Ground-penetrating radar data from the other bays indicate that the stratigraphy of all seven bays is broadly similar. We conclude t h a t (1) Significant modification of the bays, including rim development and basin infilling, occurred during the Holocene; (2) ponds on the early Holocene landscape were larger and more permanent than at present; (3) early Holocene climate, as indicated by both depositional processes and human activity, was not characterized by prolonged periods of extremely dry conditions; and (4) fluvial-centric models of terminal Pleistocene-early Holocene human adaptations require revision to include intensive use of isolated upland ponds.