Loktanella maritima sp. nov. isolated from shallow marine sediments

Abstract

An aerobic, Gram-stain-negative, non-motile bacterium, KMM 9530 T , was isolated from a sediment sample collected from the Sea of Japan seashore. Comparative 16S rRNA gene sequence analysis positioned novel strain KMM 9530 T in the genus Loktanella as a separate line adjacent to Loktanella sediminilitoris KCTC 32383 T , Loktanella tamlensis JCM 14020 T and Loktanella maricola JCM 14564 T with 98.5-98.2 % sequence similarity. Strain KMM 9530 T was characterized by its weak hydrolytic capacity and inability to assimilate most organic substrates. The major isoprenoid quinone was Q-10, polar lipids consisted of phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, an unknown phospholipid, an unknown aminolipid and unknown lipids, and the major fatty acid was C 18 : 1 v7c. On the basis of phylogenetic analysis, DNA-DNA hybridization and phenotypic characterization, it can be concluded that the novel strain KMM 9530 T represents a novel species in the genus Loktanella, for which the name Loktanella maritima sp. nov. is proposed. The type strain of the species is KMM 9530 T (5NRIC 0919 T 5JCM 19807 T ).The genus Loktanella was initially proposed by Van Trappen et al. (2004) to accommodate three species, Loktanella salsilacus, as the type species of the genus, Loktanella vestfoldensis and Loktanella fryxellensis, and subsequently emended by Moon et al. (2010), Lee (2012) and Tsubouchi et al. (2013) Here we report the polyphasic characterization of a Gramstain-negative, aerobic, beige-pigmented, non-motile bacterium, designated KMM 9530 T , which was isolated from a shallow sediment sample. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain KMM 9530 T belongs to the genus Loktanella and may represent a novel species of this genus. Differential phenotypic properties, together with phylogenetic distinctiveness and DNA relatedness, demonstrated that strain KMM 9530 T differed from related species of the genus Loktanella. On the basis of phenotypic and molecular data obtained, a novel species, Loktanella maritima sp. nov., is described..Strain KMM 9530 T was isolated from a shallow sediment sample, collected from Peter the Great Bay, the Sea of Japan, Russia, as described previously (Romanenko et al., 2004). Strain KMM 9530 T was grown aerobically on/in marine 2216 agar (MA) or marine broth (MB; BD Difco), and stored at 280 u C in MB supplemented with 30 % (v/v) glycerol. The type strains L. sediminilitoris KCTC 32383 T , L. maricola JCM 14564 T , L. tamlensis JCM 14020 T and L. rosea KMM 6003 T were kindly provided by the respective culture collections and used in the phenotypic and lipids analyses. Gram staining, oxidase and catalase reactions, and motility (hanging drop method) were tested as described by Gerhardt et al. (1994). The morphology of cells negatively stained with 1 % phosphotungstic acid was examined by electronic transmission microscopy [Libra 200Abbreviations: ASW, artificial seawater; DPG, diphosphatidylglycerol; PC, phosphatidylcholine; PG, phosphatidylglycerol.The GenB...

Loktanella sediminum sp. nov., isolated from marine surface sediment

“…Differences in fatty acid compositions and the proportion of some components were identified between strain S3B03 T and the two reference strains (). The predominant isoprenoid quinone detected in strain S3B03 T was Q-10, in line with other species of the genus Loktanella (Weon et al , 2006; Yoon et al , 2007, 2013; Hosoya & Yokota, 2007; Moon et al , 2010; Lee, 2012; Tsubouchi et al , 2013; Park et al , 2013a, b; Tanaka et al , 2014). The major polar lipids detected in strain S3B03 T were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine and two unidentified polar lipids (Fig.…”

“…The genus description was subsequently emended by Moon et al (2010), Lee (2012) and Tsubouchi et al (2013). At the time of writing, the genus Loktanella comprised 16 species with validly published names: L. salsilacus , L. fryxellensis and L. vestfoldensis (Van Trappen et al , 2004), L. hongkongensis (Lau et al , 2004), L. agnita and L. rosea (Ivanova et al , 2005), L. koreensis (Weon et al , 2006), L. maricola (Yoon et al , 2007), L. atrilutea (Hosoya & Yokota, 2007), L. pyoseonensis (Moon et al , 2010), L. tamlensis (Lee, 2012), L. litorea (Yoon et al , 2013), L. cinnabarina (Tsubouchi et al , 2013), L. sediminilitoris (Park et al , 2013a), L. soesokkakensis (Park et al , 2013b) and L. maritima (Tanaka et al , 2014). These bacteria have been isolated from various sources, including water, sediment, sand, marine biofilms, etc.…”

Microbial Communities on Seafloor Basalts at Dorado Outcrop Reflect Level of Alteration and Highlight Global Lithic Clades

“…Another Alphaproteobacteria clade containing Otu000075 within the Rhodobacterales also has clones from Vailulu'u, Loihi, Lau Basin, EPR, and 9N. These are most closely related to the cultured representative Loktanella maritima (96% similarity), which was recently isolated from shallow sediments in the Sea of Japan (Tanaka et al, 2014 ). Within the Rhizobiales order there are numerous clades branching out around the cultured Hyphomicrobium vulgare , containing sequences recovered from every site except the very young Vailulu'u and 9N samples (being from recent eruptions about 10 and 20 years ago, respectively).…”

Draft Genome Sequence of Loktanella maritima Strain YPC211, a Commensal Bacterium of the American Lobster (Homarus americanus)

“… Loktanella maritima is a Gram-negative aerobic bacterium that was originally isolated from shallow marine sediments in the Sea of Japan ( 1 ). Loktanella spp.…”