Lolium perenne'de Fosfat Eksikliği Stresinde miRNA408’in Rolü: Üç Yıllık Tarla Çalışması

Abstract

Phosphate deficiency is a stress factor limiting crop growth and development. Under phosphate limited conditions, crops have developed a variety of molecular strategies. miRNAs (miRs) are characterized as a regulator of main processes such as stress mechanisms in plants by silencing genes. miR408 has role in mediating plant responses to phosphate starvation in limited number of plants. The miRs involved in the phosphate deficiency mechanism in Lolium perenne (perennial ryegrass) remain unelucidated. The aim of this study was to confirm the presence of miR408 expression in perennial ryegrass and control whether it plays an important role to protect against phosphate deficiency stress in field conditions. The sensitivities (hay yield and quality features) of four perennial ryegrass populations against phosphate deficiency stress were determined by three-year field experiment. The results revealed that the declines on hay yield and quality features were less pronounced for P1 and P4 populations compared to others (P2, and P3) against phosphate deficiency stress. Molecular analysis showed that significant up-regulations were observed in the expression level of the miR408 in P1 and P4, while no changes were observed in P2, and P3. The results collectively suggest that miR408 could be responsible for the tolerance in phosphate-limited conditions in perennial ryegrass. This miR could be used for the development of perennial ryegrass plants that are tolerant to phosphate-deficient soils.

A Comparison On The Virtue Of Temperance/Iffah (Chastity) From Ancient Greek To Islamic Thought

“…Biyolojik olarak çocuğun gelişim evreleri dikkate alındığında iffet, şecaat ve hikmet erdemlerinin sırasıyla ortaya çıktığı söylenebilir. 74 Bu bağlamda İsfahânî'nin bu değerlendirmesi, iffet erdeminin diğer erdemlerin teşekkülünde oynayabileceği role bir işaret olarak algılanabilir.…”