lon transcriptional regulation of genes necessary for capsular polysaccharide synthesis in Escherichia coli K-12

Trisler, P; Gottesman, Susan

doi:10.1128/jb.160.1.184-191.1984

Cited by 145 publications

(34 citation statements)

References 35 publications

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“…However, as others have observed (Holder et al, 1985), some of them are subject to proteolysis. In our hands, proteasedeficient strains (Trisler and Gottesmann, 1984) did not significantly improve the expression yield or stability of these proteins.…”

Section: Resultscontrasting

confidence: 48%

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Major surface antigen p190 of Plasmodium falciparum: detection of common epitopes present in a variety of plasmodia isolates.

et al. 1988

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Plasmodium falciparum merozoites are covered with polymorphic proteins that are processed from a 190 kd (p190) precursor protein. These are candidates for an antimalarial vaccine. We cloned and expressed a number of DNA fragments, comprising almost the entire p190 gene of the K1 isolate, in Escherichia coli. Pooled human endemic‐area sera and rabbit antibodies raised against p190 protein isolated from K1 parasites react with only a limited number of the recombinant proteins. From these studies we could select two antigenic polypeptides containing conserved amino acid stretches of the otherwise highly polymorphic protein. Rabbits and mice injected with the purified recombinant proteins produce antibodies reacting differentially with various isolates of P. falciparum. We obtained antibodies detecting all isolates tested and a monoclonal antibody specific for isolates containing a K1 type allele of the p190 gene.

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Section: Resultscontrasting

confidence: 48%

“…For DNA transformations the E. coli K12 strain HB 101 was used. Recombinant proteins were expressed in E. coli strains M15 (Certa et al, 1986) or SG 200-50 (Ion A-; Trisler and Gottesmann, 1984).…”

Section: Bacteriamentioning

confidence: 99%

Major surface antigen p190 of Plasmodium falciparum: detection of common epitopes present in a variety of plasmodia isolates.

et al. 1988

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“…To clarify this role, two mutants were constructed: (i) a mutant with abolished CA synthesis was constructed by transduction of a knock-out mutation in the cpsE (i.e. wcaE) gene resulting in the disruption of CA operon transcription (Trisler and Gottesman, 1984;Stevenson et al, 1996), and (ii) a mutant overproducing CA, obtained by deletion of the lon gene (Trisler and Gottesman, 1984). PHL644 and these two strains were grown on M63 glucose agar plates to verify their CA production.…”

Section: Role Of Motility In Biofilm Formationmentioning

confidence: 99%

Developmental pathway for biofilm formation in curli‐producing Escherichia coli strains: role of flagella, curli and colanic acid

Prigent‐Combaret

Prensier

Thi

et al. 2000

Environmental Microbiology

411

340

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This work was performed to establish a model describing bacterial surface structures involved in biofilm development, in curli-overproducing Escherichia coli K-12 strains, at 30 degrees C, and in minimal growth medium. Using a genetic approach, in association with observations of sessile communities by light and electron microscopic techniques, the role of protein surface structures, such as flagella and curli, and saccharidic surface components, such as the E. coli exopolysaccharide, colanic acid, was determined. We show that, in the context of adherent ompR234 strains, (i) flagellar motility is not required for initial adhesion and biofilm development; (ii) both primary adhesion to inert surfaces and development of multilayered cell clusters require curli synthesis; (iii) curli display direct interactions with the substratum and form interbacterial bundles, allowing a cohesive and stable association of cells; and (iv) colanic acid does not appear critical for bacterial adhesion and further biofilm development but contributes to the biofilm architecture and allows for the formation of voluminous biofilms.

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“…Briefly, at least six genes required for the synthesis of colanic acid {ops) are controlled by tv^fo negative regulators, lon and rcsC, and two positive regulators, rcsA and rcsB {Gottesman and Zipser. 1978: Gottesman et al, 1985;Trisler and Gottesman, 1984). rcsB controls the transcription of the structural genes involved in the biosynthesis of colanic acid.…”

Section: Introductionmentioning

confidence: 99%

Positive control of colanic acid synthesis in Escherichia coli by rmpA and rmpB, two virulence‐plasmid genes of Kiebsiella pneumoniae

Nassif

Honoré

Vasselon

et al. 1989

Molecular Microbiology

101

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In Klebsiella pneumoniae, the mucoid phenotype, which is a virulence factor, is distinct from capsule production. It is positively controlled by a plasmid gene, designated rmpA. When introduced into certain Escherichia coli strains, rmpA induces expression of a mucoid phenotype, which results from overproduction of colanic acid at 30 degrees C but not at 37 degrees C. In E. coli, production of colanic acid is regulated by three genes: rcsA and rcsB which act as positive regulators, and rcsC which is a negative effector. In this work we present evidence that the rmpA gene complemented an rcsA, Ion double mutant of E. coli, but not an rcsA, Ion+ isolate. This leads to the suggestion that rmpA expressed an rcsA-like activity and like rcsA, was negatively controlled at post-transcriptional level by the Lon protease. The nucleotide sequence of rmpA is reported. No homology could be found between the 27 kiloDalton RcsA protein and the deduced amino acid sequence of the 15.5 kiloDalton RmpA protein. Another gene, rmpB, which was required in E. coli recA isolates for full expression of rmpA at 30 degrees C, has been identified on the K. pneumoniae virulence plasmid and shown to encode a 37 kiloDalton protein. Although rmpB was closely linked to rmpA, it was not present on the same transcriptional unit. These results suggested that induction of colanic acid synthesis by the K. pneumoniae virulence gene rmpA, was, at least in E. coli, under the control of the RecA network via rmpB, which may act as a positive regulator of rmpA. We conclude that these plasmid genes may function in K. pneumoniae as regulatory genes controlling the mucoid phenotype, which is itself encoded by the chromosome.

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lon transcriptional regulation of genes necessary for capsular polysaccharide synthesis in Escherichia coli K-12

Cited by 145 publications

References 35 publications

Major surface antigen p190 of Plasmodium falciparum: detection of common epitopes present in a variety of plasmodia isolates.

Major surface antigen p190 of Plasmodium falciparum: detection of common epitopes present in a variety of plasmodia isolates.

Developmental pathway for biofilm formation in curli‐producing Escherichia coli strains: role of flagella, curli and colanic acid

Positive control of colanic acid synthesis in Escherichia coli by rmpA and rmpB, two virulence‐plasmid genes of Kiebsiella pneumoniae

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