Long chain base changes triggered by a short exposure of Arabidopsis to low temperature are altered by AHb1 non-symbiotic haemoglobin overexpression

Guillas, Isabelle; Guellim, Amira; Rézé, Nathalie; Baudouin, Emmanuel

doi:10.1016/j.plaphy.2012.11.020

Cited by 28 publications

(28 citation statements)

References 31 publications

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“…PCD induction by long-chain-base accumulation appears to be the mode of action for sphinganine analog mycotoxins, including fumonisin B 1 (FB 1 ) and AAL toxin (Abnet et al, 2001;Brandwagt et al, 2002). In addition, phosphorylated forms of long-chain bases (LCBs) have been implicated in abscisic acid-dependent guard cell closure and low temperature signaling in plants (Coursol et al, 2003(Coursol et al, , 2005Chen et al, 2012;Guillas et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Arabidopsis56–Amino Acid Serine Palmitoyltransferase-Interacting Proteins Stimulate Sphingolipid Synthesis, Are Essential, and Affect Mycotoxin Sensitivity

et al. 2013

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Maintenance of sphingolipid homeostasis is critical for cell growth and programmed cell death (PCD). Serine palmitoyltransferase (SPT), composed of LCB1 and LCB2 subunits, catalyzes the primary regulatory point for sphingolipid synthesis. Small subunits of SPT (ssSPT) that strongly stimulate SPT activity have been identified in mammals, but the role of ssSPT in eukaryotic cells is unclear. Candidate Arabidopsis thaliana ssSPTs, ssSPTa and ssSPTb, were identified and characterized. Expression of these 56-amino acid polypeptides in a Saccharomyces cerevisiae SPT null mutant stimulated SPT activity from the Arabidopsis LCB1/LCB2 heterodimer by >100-fold through physical interaction with LCB1/LCB2. ssSPTa transcripts were more enriched in all organs and >400-fold more abundant in pollen than ssSPTb transcripts. Accordingly, homozygous ssSPTa T-DNA mutants were not recoverable, and 50% nonviable pollen was detected in heterozygous ssspta mutants. Pollen viability was recovered by expression of wild-type ssSPTa or ssSPTb under control of the ssSPTa promoter, indicating ssSPTa and ssSPTb functional redundancy. SPT activity and sensitivity to the PCD-inducing mycotoxin fumonisin B 1 (FB 1 ) were increased by ssSPTa overexpression. Conversely, SPT activity and FB 1 sensitivity were reduced in ssSPTa RNA interference lines. These results demonstrate that ssSPTs are essential for male gametophytes, are important for FB 1 sensitivity, and limit sphingolipid synthesis in planta.

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Section: Introductionmentioning

confidence: 99%

Arabidopsis56–Amino Acid Serine Palmitoyltransferase-Interacting Proteins Stimulate Sphingolipid Synthesis, Are Essential, and Affect Mycotoxin Sensitivity

et al. 2013

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“…Indeed, an Arabidopsis transgenic line overexpressing the non-symbiotic haemoglobin AHb1 presented less root growth inhibition when cultured at 12 °C than wild type (Guillas et al 2012). Moreover, this mutant did not accumulate anthocyanin when cultivated at 4 °C (Guillas et al 2012). Together with a strong impairment of cold-responsive gene expression in AHb1 overexpressor (see next paragraphs), these data suggest that NO is required for an array of cold-induced responses.…”

Section: No and Plant Tolerance To Low Temperaturementioning

confidence: 86%

“…Recent data also indicate that NO participates in chilling response. Indeed, an Arabidopsis transgenic line overexpressing the non-symbiotic haemoglobin AHb1 presented less root growth inhibition when cultured at 12 °C than wild type (Guillas et al 2012). Moreover, this mutant did not accumulate anthocyanin when cultivated at 4 °C (Guillas et al 2012).…”

Section: No and Plant Tolerance To Low Temperaturementioning

confidence: 98%

Nitric Oxide as a Mediator of Cold Stress Response: A Transcriptional Point of View

Baudouin

Jeandroz

2015

Nitric Oxide Action in Abiotic Stress Responses in Plants

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Low temperature constitutes a major constraint for plant development and spreading and imprints plant biodiversity. Plant tolerance towards cold is a complex matter that relies on deep metabolic reprogramming principally governed by transcriptomic changes themselves controlled through multiple signalling pathways. A set of recent reports points out nitric oxide (NO) as a key element of signalling networks underlying plant response to low temperature. Based on the identification of several cold-regulated NO-dependent genes, that play key functions during plant response to temperature lowering, NO might sustain major functions during cold stress. This chapter summarizes our current knowledge on NO availability and bioactivity during low-temperature response, with a special emphasis on its implication in the regulation of gene expression.

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“…Secondly NO might modify the availability of LCB/Cer kinase substrates. Supporting this possibility, Guillas et al (2013) evidenced that an Arabidopsis mutant line over-expressing a non-symbiotic hemoglobin, and thereby exhibiting low NO levels, over-accumulates phytosphingosine. The levels of phytosphingosine were further increased after cold exposure and might afford for the highest rate of phytosphingosine-P formation observed in this mutant (Cantrel et al, 2011).…”

Section: Interplays Between Sl and No Signaling In Plants: Promises Fmentioning

confidence: 96%

“…Interestingly, the analysis revealed another facet of the SL response affected in this mutant. Indeed, whereas the overall amount of LCB was strongly lowered by cold exposure in WT plants, it was drastically increased in the mutant line (Guillas et al, 2013). These data therefore suggest that NO might participate in the regulation of more complex sphingolipidome modifications associated with cold response.…”

Section: Interplays Between Sl and No Signaling In Plants: Promises Fmentioning

confidence: 98%

Nitric oxide-sphingolipid interplays in plant signalling: a new enigma from the Sphinx?

Guillas¹,

Puyaubert²,

Baudouin³

2013

Front. Plant Sci.

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Nitric oxide (NO) emerged as one of the major signaling molecules operating during plant development and plant responses to its environment. Beyond the identification of the direct molecular targets of NO, a series of studies considered its interplay with other actors of signal transduction and the integration of NO into complex signaling networks. Beside the close relationships between NO and calcium or phosphatidic acid signaling pathways that are now well-established, recent reports paved the way for interplays between NO and sphingolipids (SLs). This mini-review summarizes our current knowledge of the influence NO and SLs might exert on each other in plant physiology. Based on comparisons with examples from the animal field, it further indicates that, although SL–NO interplays are common features in signaling networks of eukaryotic cells, the underlying mechanisms and molecular targets significantly differ.

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Long chain base changes triggered by a short exposure of Arabidopsis to low temperature are altered by AHb1 non-symbiotic haemoglobin overexpression

Cited by 28 publications

References 31 publications

Arabidopsis56–Amino Acid Serine Palmitoyltransferase-Interacting Proteins Stimulate Sphingolipid Synthesis, Are Essential, and Affect Mycotoxin Sensitivity

Arabidopsis56–Amino Acid Serine Palmitoyltransferase-Interacting Proteins Stimulate Sphingolipid Synthesis, Are Essential, and Affect Mycotoxin Sensitivity

Nitric Oxide as a Mediator of Cold Stress Response: A Transcriptional Point of View

Nitric oxide-sphingolipid interplays in plant signalling: a new enigma from the Sphinx?

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