Long-circulating polyhydroxybutyrate-co-hydroxyvalerate nanoparticles for tumor targeted docetaxel delivery: Formulation, optimization and in vitro characterization

Vardhan, Harsh; Moteriya, Pooja; Adena, Sandeep Kumar Reddy; Mishra, Brahmeshwar

doi:10.1016/j.ejps.2016.12.007

Cited by 45 publications

(27 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…High-performance liquid chromatography with reverse phase was used for quantification of Gen in the prepared NLC formulation. HPLC system was consisted of waters 1525 binary HPLC pump (Waters, USA), rheodyne 7725i manual injector (Waters, USA), C18 reverse-phase (4.6 x75 mm; 3.5 µm) Symmetry® C18 column and waters 2998 photodiode array detector (Waters, USA) 14,29,30 . Acetonitrile: 2mM phosphoric acid buffer in Milli-Q water (50:50) was used as mobile phase and was run at a rate of 1.0 ml/min.…”

Section: Characterization Tests Hplc Methods Developmentmentioning

confidence: 99%

“…of experimental runs even with large no. of factors 12,14 . The factors were studied at two levels i.e.…”

Section: Screening Of Factors By Placket Burman Designmentioning

confidence: 99%

“…To differentiate the interactive effects of 2 independent variables on dependent variable, we had plotted 3D surface contour plots which are shown in Figure 7. Results of the quadratic models analysis were depicted in the form of lack of fit value as well as p-value 14,15,28 Influence of variables on particle size: Particle size of GENNLC varied from 119.3 to 383.9 nm during all the experimental runs. The F value of 184.04 allowed sufficient fitting of data with nonsignificant lack of fit (0.1998).…”

Section: The Placket Burman Design (Pbd)mentioning

confidence: 99%

“…In short, QbD is a methodical approach which utilizes the principles of statistics and DoE and the promises to produce the high quality of the product which satisfies the customer needs in all respect. The various steps of DoE include: (a) define objective (b) identify critical quality attributes (c) screening of most influencing factors (d) experimental design (e) analysis of responses (f) optimization process (g) validation of methodology 6,9,[12][13][14][15] . Despite various designs available, we had selected Placket Burman (PB) factorial based design for the screening of large no.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Genistein-Loaded Nanostructured Lipid Carriers for Intravenous Administration: A Quality by Design Based Approach

Mittal¹,

Vardhan²,

Ajmal³

et al. 2019

Int. Res. J. Pharm.

Self Cite

View full text Add to dashboard Cite

Genistein (Gen) is a naturally occurring soy isoflavonoid, ing anticancer, antiproliferation & antioxidant-like properties which make it suitable as an anticancer medicine. The disadvantage of poor solubility and less oral bioavailability restrict its use as potential anticancer agent. In the present study, Gen was entrapped into solid-liquid lipid mixture with the aid of surfactant by using modified solvent evaporation technique. The present work was carried out with the aim to screen the component as well as process variables to ease the formulation process which ends up with the finished product having characteristics of best nanoformulation in all respects. Optimized levels by employing numerical optimization technique for each factor viz. surfactant concentration (X1), Lipid concentration (X2) & amount of organic solvent (X3) were 0.3 %, 0.78 % & 8.51 ml respectively. The resultant formulation exhibited a particle size of 122.22 nm, and entrapment efficiency of 92.8 %, & zeta potential of-21.25 mV with unimodal size distribution. Findings of the haemocompatibility studies suggested that optimized formulation was pretty safe for intravenous administration. In a nutshell, GenNLC seems to be a superior alternative carrier system for the formulation industry to obtain the higher entrapment with excellent stability of the formulation.

show abstract

Section: Characterization Tests Hplc Methods Developmentmentioning

confidence: 99%

“…of experimental runs even with large no. of factors 12,14 . The factors were studied at two levels i.e.…”

Section: Screening Of Factors By Placket Burman Designmentioning

confidence: 99%

Section: The Placket Burman Design (Pbd)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Genistein-Loaded Nanostructured Lipid Carriers for Intravenous Administration: A Quality by Design Based Approach

Mittal¹,

Vardhan²,

Ajmal³

et al. 2019

Int. Res. J. Pharm.

Self Cite

View full text Add to dashboard Cite

show abstract

“…The measurements were also repeated at 3 rd and 6 th months for stability study. Encapsulation efficiency were calculated using the following Equation 4 16 .…”

Section: Determination Of Olo In Polymeric Nanoparticlesmentioning

confidence: 99%

Development and validation of uplc method for the determination of olopatadine hydrochloride in polymeric nanoparticles

Güven¹,

Berkman²,

Yazan³

2019

actapharm

View full text Add to dashboard Cite

A new, simple, rapid, precise, accurate and specific stability indicating reverse phase UPLC method developed for the determination of encapsulated olopatadine hydrochloride (OLO) in the polymeric nanoparticle formulations. Studies were carried out on a 2.1x50 mm, 1.8 µm Zorbax Eclipse Plus C 18 column with an optimized mobile phase of methanol, water and sodium acetate buffer solution (40:50:10, v/v/v) at a flow rate of 0.5 mL/min. OLO was detected and quantitated using a photodiode array detector at a wavelength of 246 nm and the column temperature was adjusted to 40 °C. The proposed method validation was carried out for specificity, linearity, accuracy, precision, limit of detection, limit of quantitation and robustness according to the ICH harmonised tripartite guideline "validation of analytical procedures Q2(R1)". Analytical curve was linear over the concentration range of 5-50 µg/mL. All the validation parameters were within the acceptance range. LOD and LOQ for OLO were 0.7652 and 2.3188 µg/mL, respectively. The developed method fulfilled the requirements for reliability and feasibility for the quantitative analysis of OLO in polymeric nanoparticles.

show abstract

Doxorubicin/poly(caprolactone)/polyethylene glycol nanoparticles prepared by electrospray

Mehrazin,

Asefnejad,

Naeimi

et al. 2023

Polymers for Advanced Techs

View full text Add to dashboard Cite

The development of sustainable drug delivery systems is very important in the field of pharmaceutical research and has many advantages over the usual methods of drug administration, including improving effectiveness, reducing side effects, and increasing patient compliance. Among various techniques, electrospraying as a voltage‐driven process has emerged as a promising technology for the fabrication of drug‐loaded nanoparticles. In recent years, the application of electrospray approach for drug release has been widely investigated. However, optimization of electrospray process parameters and development of mathematical models to predict drug release kinetics from microparticles are still challenging. To address these issues, a combination of Central Composite Design (CCD) and Response Surface Methodology (RSM) has been used. This paper focuses on the application of CCD‐RSM for modeling and optimization of doxorubicin (DOX)‐poly(caprolactone) (PCL)‐polyethylene glycol (PEG) microparticles prepared using electrospray method. Doxorubicin, a widely used anticancer drug, exhibits potent cytotoxicity against a wide range of cancers. PCL‐PEG microparticles are promising as drug carriers due to their biocompatibility, controlled release properties, and ability to protect the encapsulated drug from degradation. The CCD‐RSM approach allows systematic investigation and optimization of several formulation and process variables, including polymer concentration, PCL‐PEG weight ratio, and applied voltage on drug release behavior. In addition, the effect of rheology and electrical conductivity of polymer fluid on the size of particles obtained from electrospraying was investigated. The effect of adding folic acid and doxorubicin in the optimized sample of nanoparticles and the effect of different concentrations of doxorubicin were investigated, and SEM, DLS, and FTIR techniques were used to characterize the microparticles, and the MMT test was performed to evaluate their cytotoxicity. The results showed that the optimized formulation is effective for sustained drug release over a long period for drug delivery applications.

show abstract

Long-circulating polyhydroxybutyrate-co-hydroxyvalerate nanoparticles for tumor targeted docetaxel delivery: Formulation, optimization and in vitro characterization

Cited by 45 publications

References 31 publications

Genistein-Loaded Nanostructured Lipid Carriers for Intravenous Administration: A Quality by Design Based Approach

Genistein-Loaded Nanostructured Lipid Carriers for Intravenous Administration: A Quality by Design Based Approach

Development and validation of uplc method for the determination of olopatadine hydrochloride in polymeric nanoparticles

Doxorubicin/poly(caprolactone)/polyethylene glycol nanoparticles prepared by electrospray

Contact Info

Product

Resources

About