Long Distance Charge Transport through DNA: Quantification and Extension of the Hopping Model

Giese, Bernd; Spichty, Martin

doi:10.1002/1439-7641(20001215)1:4<195::aid-cphc195>3.0.co;2-b

Cited by 140 publications

(45 citation statements)

References 30 publications

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“…Hydration of the guanine radical cation [10] occurs with a pseudo-first-order rate constant estimated to be 6 × 10 4 s -1 within double-stranded DNA [11] giving rise to 8-hydroxy-7,8-dihydroguanyl radical (Fig. 2).…”

Section: One-electron Oxidation Reactionsmentioning

confidence: 99%

UVB and UVA radiation-mediated damage to isolated and cellular DNA

Cadet

Courdavault

Ravanat

et al. 2005

Pure and Applied Chemistry

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Abstract:The effects of solar light on cellular DNA are mostly explained by both direct excitation of nucleobases and photosensitized reactions that are mediated by UVB and UVA radiation, respectively. A large body of information is now available on the main photodynamic reactions to DNA, which involve guanine as the preferential target of both one-electron oxidation and singlet oxygen oxidation, as the result of type I and type II mechanisms. Most of the final products of the photosensitized reactions of guanine base in isolated DNA have been characterized, and comprehensive mechanisms for their formation have been proposed. Further insights into the mechanisms of solar radiation-induced modifications within cellular DNA have been gained from accurate measurement of the main photoproducts using recently designed sensitive chromatographic and biochemical methods. Thus, the distribution pattern of the 12 possible bipyrimidine photoproducts has been shown to be similar in the DNA of UVB-irradiated rodent and human cells. Cyclobutyl pyrimidine dimers are also generated at di-thymine and thymine-cytosine sites within nuclear DNA upon exposure to UVA radiation as the likely result of triplet energy transfer. In addition, oxidative reactions that involved mostly singlet oxygen, and to a lesser extent, ؒ OH radicals are also implicated, although less efficiently.

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Section: One-electron Oxidation Reactionsmentioning

confidence: 99%

UVB and UVA radiation-mediated damage to isolated and cellular DNA

Cadet

Courdavault

Ravanat

et al. 2005

Pure and Applied Chemistry

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“…through the processes of DNA-damage sensing or repairing via long-range charge transfer [6][7]. The prevailing DNA architecture, the double helix, has well stacked, nearly parallel bases with overlapping π-electron systems.…”

Section: Introductionmentioning

confidence: 99%

Conductivity of natural and modified DNA measured by scanning tunneling microscopy. The effect of sequence, charge and stacking

Kratochvílová¹,

Král²,

Bunček³

et al. 2008

Biophysical Chemistry

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT AbstractThe conductivity of DNA covalently bonded to a gold surface was studied by means of the STM technique. Various single-and double-stranded 32-nucleotide-long DNA sequences were measured under ambient conditions so as to provide a better understanding of the complex process of chargecarrier transport in natural as well as chemically modified DNA molecules. The investigations focused on the role of several features of DNA structure, namely the role of the negative charge at the backbone phosphate group and the related complex effects of counterions, and of the stacking interactions between the bases in Watson-Crick and other types of base pairs. The measurements have indicated that the best conductor is DNA in its biologically most relevant double-stranded form with Watson-Crick base pairs and charged phosphates equilibrated with counterions and water. All the studied modifications, including DNA with non-Watson-Crick base pairs, the abasic form, and especially the form with phosphate charges eliminated by chemical modifications, lower the conductivity of natural DNA.

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“…The pioneering work of Jacqueline Barton and coworkers who studied photoactivated charge transfer in small DNA fragments (Turro and Barton, 1998;Wan et al, 1999;Kelly and Barton, 1999;Núnez et al, 2001) and Bernd Giese and coworkers (Giese and Spichty, 2000;Giese et al, 2001;Meggers et al, 1998;Danith et al, 1999) who investigated the charge hopping of free cationic radicals along the distance of several base pairs of DNA fostered a very actual field. The investigation of long-range charge transport in DNA molecules were first reported by Fink and Schönenberger (1999) who found considerable charge transport by probing individual DNA molecules in vacuum by a low energy electron point source.…”

Section: Introductionmentioning

confidence: 99%

Absence of intrinsic electric conductivity in single dsDNA molecules

Kleine¹,

Wilke²,

Pelargus³

et al. 2004

Journal of Biotechnology

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The intrinsic dc conductivity of long, individual lambda phage dsDNA molecules has been investigated by ultrasensitive low current-voltage-spectroscopy (IV) under ambient conditions and controlled low humidity inert gas atmosphere on microfabricated metal-insulator-metal gap structures. We found a strong dependence of the measured conductivity on the apparent humidity, which we attribute to capillary condensation of water to the immobilized DNA molecules, giving rise to additional ionic currents. Additional IV-spectroscopy experiments under controlled argon atmosphere always revealed a significant drop in electrical conductivity to 4 × 10 −15 A V −1 m −1 , indicating almost no considerable contribution of electrical long range charge transport.

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Long Distance Charge Transport through DNA: Quantification and Extension of the Hopping Model

Cited by 140 publications

References 30 publications

UVB and UVA radiation-mediated damage to isolated and cellular DNA

UVB and UVA radiation-mediated damage to isolated and cellular DNA

Conductivity of natural and modified DNA measured by scanning tunneling microscopy. The effect of sequence, charge and stacking

Absence of intrinsic electric conductivity in single dsDNA molecules

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