Unmanned aerial vehicles (UAVs) visual object tracking under low-light conditions serves as a crucial component for applications, such as night surveillance, indoor searches, night combat, and all-weather tracking. However, the majority of the existing tracking algorithms are designed for optimal lighting conditions. In low-light environments, images captured by UAV typically exhibit reduced contrast, brightness, and a signal-to-noise ratio, which hampers the extraction of target features. Moreover, the target’s appearance in low-light UAV video sequences often changes rapidly, rendering traditional fixed template tracking mechanisms inadequate, and resulting in poor tracker accuracy and robustness. This study introduces a low-light UAV object tracking algorithm (SiamLT) that leverages image feature enhancement and a dynamic template-updating Siamese network. Initially, the algorithm employs an iterative noise filtering framework-enhanced low-light enhancer to boost the features of low-light images prior to feature extraction. This ensures that the extracted features possess more critical target characteristics and minimal background interference information. Subsequently, the fixed template tracking mechanism, which lacks adaptability, is enhanced by dynamically updating the tracking template through the fusion of the reference and base templates. This improves the algorithm’s capacity to address challenges associated with feature changes. Furthermore, the Average Peak-to-Correlation Energy (APCE) is utilized to filter the templates, mitigating interference from low-quality templates. Performance tests were conducted on various low-light UAV video datasets, including UAVDark135, UAVDark70, DarkTrack2021, NAT2021, and NAT2021L. The experimental outcomes substantiate the efficacy of the proposed algorithm in low-light UAV object-tracking tasks.