Long-distance signalling of abscisic acid (ABA): the factors regulating the intensity of the ABA signal

Jiang, Fan; Hartung, Wolfram

doi:10.1093/jxb/erm127

Cited by 256 publications

(198 citation statements)

References 26 publications

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“…Relatively high levels of ABA were detected in the spent media, as compared with most of the tested CK derivatives, with the only exception being methylthiols. The observed specific ABA distribution between Euglena cells and the slightly alkaline growth medium (pH 7.5) is in accordance with the previous report, that ABA transport in vascular plants is regulated by apoplastic pH of the leaf and stem and its flow in the plant intensifies with the increasing pH of the tissue (Jiang & Hartung, 2008).…”

Section: Discussionsupporting

confidence: 92%

“…Abscisic acid (ABA) is a phytohormone that plays an important role in plant responses to environmental stress and plant pathogens (Jiang & Hartung, 2008;Santner et al, 2009). The general functions of ABA in vascular plants are to inhibit precocious germination and promote dormancy in seeds, and production of molecules that protect cells against desiccation (Nakashima & Yamaguchi-Shinozaki, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Euglena gracilis(Euglenophyceae) produces abscisic acid and cytokinins and responds to their exogenous application singly and in combination with other growth regulators

Noble

Kisiała

Galer

et al. 2014

European Journal of Phycology

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The main objective of this study was to determine the optimal concentrations of a wide spectrum of exogenous phytohormones for effective stimulation of cell division and production of maximum cell yield in Euglena gracilis Klebs cultured in vitro. Results indicate that two hormones combined exert more effective growth stimulation than a single hormone or three, four or five different hormones combined. Specifically, trans-zeatin at 10 −7 M combined with abscisic acid at 10 −9 M produced optimal conditions for growth, yielding the maximum cell concentration. High concentrations of exogenous phytohormones were toxic to Euglena. The addition of trans-zeatin, N6-isopentenyladenine, and benzylaminopurine to Euglena cultures resulted in dense, dark green chloroplasts, suggesting that exogenous phytohormones increased the production of chlorophyll. Given the response to exogenous growth regulators, the study identified and quantified the types of endogenous cytokinins (CKs) and abscisic acid (ABA) synthesized in vitro by Euglena gracilis. HPLC-(ESI) MS/MS analysis revealed that the algal cells produced and released into the medium a mixture of CKs and ABA. The main CKs identified in the cell pellets and supernatant samples were from a t-RNA degradation pathway and included: cis-zeatin (cZ) derivatives cZR, cZNT, MeSZ and MeSZR, and to a lesser extent, the free base N6-isopentenyladenine (iP) and its derivatives iPR, iPNT, MeSiP and MeSiPA. A positive response to ABA, and the relatively high levels detected in E. gracilis, suggest that this hormone is important for alleviating stress conditions of in vitro culture that might otherwise restrict cell division.Key words: abscisic acid, algal growth, cell morphology, cytokinin, phytohormone treatment, high performance liquid chromatography mass spectrometry

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Euglena gracilis(Euglenophyceae) produces abscisic acid and cytokinins and responds to their exogenous application singly and in combination with other growth regulators

Noble

Kisiała

Galer

et al. 2014

European Journal of Phycology

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“…In response, patterns of root growth are altered, with some root tips inhibited and others stimulated, and stomata in the leaf close (Daszkowska-Golec and Szarejko, 2013;Harris, 2015). The rapid increase in xylem sap ABA in response to salinity is thought to be due to multiple sources: increased biosynthesis, phloem import of ABA from the leaves to the roots, and release of ABA from the conjugated form, ABAglucose ester (ABA-GE) (Jiang and Hartung, 2008).…”

Section: Introductionmentioning

confidence: 99%

“…Unlike jasmonic acid, which is most active when conjugated to isoleucine (Staswick and Tiryaki, 2004), ABA conjugates are thought to be inactive forms, which are stored in the vacuole and transported in the xylem (Jiang and Hartung, 2008). In response to salt stress, ABA can be rapidly released from ABA-GE by b-glucosidases in the endoplasm reticulum (ER) or the cell wall space (Dietz et al, 2000;Lee et al, 2006) In Arabidopsis, b-GLUCOSIDASE1 (BG1) has been shown to function in the ER to release ABA from ABA-GE stores in response to salt stress (Lee et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Environmental Nitrate Stimulates Abscisic Acid Accumulation in Arabidopsis Root Tips by Releasing It from Inactive Stores

2016

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Abscisic acid (ABA) signaling plays a major role in root system development, regulating growth and root architecture. However, the precise localization of ABA remains undetermined. Here, we present a mechanism in which nitrate signaling stimulates the release of bioactive ABA from the inactive storage form, ABA-glucose ester (ABA-GE). We found that ABA accumulated in the endodermis and quiescent center of Arabidopsis thaliana root tips, mimicking the pattern of SCARECROW expression, and (to lower levels) in the vascular cylinder. Nitrate treatment increased ABA levels in root tips; this stimulation requires the activity of the endoplasmic reticulum-localized, ABA-GE-deconjugating enzyme b-GLUCOSIDASE1, but not de novo ABA biosynthesis. Immunogold labeling demonstrated that ABA is associated with cytoplasmic structures near, but not within, the endoplasmic reticulum. These findings demonstrate a mechanism for nitrate-regulated root growth via regulation of ABA accumulation in the root tip, providing insight into the environmental regulation of root growth.

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“…Long-distance transport of ABA from roots to leaves has been extensively discussed in relation to stomatal closure in response to water deficit (7)(8)(9)(10)(11). Stomata closed when a part of the root system was exposed to water stress, whereas the leaf water status was unchanged (11).…”

mentioning

confidence: 99%

Identification of an abscisic acid transporter by functional screening using the receptor complex as a sensor

Kanno

Hanada

Chiba

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

429

378

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Movement of the plant hormone abscisic acid (ABA) within plants has been documented; however, the molecular mechanisms that regulate ABA transport are not fully understood. By using a modified yeast two-hybrid system, we screened Arabidopsis cDNAs capable of inducing interactions between the ABA receptor PYR/PYL/ RCAR and PP2C protein phosphatase under low ABA concentrations. By using this approach, we identified four members of the NRT1/PTR family as candidates for ABA importers. Transport assays in yeast and insect cells demonstrated that at least one of the candidates ABA-IMPORTING TRANSPORTER (AIT) 1, which had been characterized as the low-affinity nitrate transporter NRT1.2, mediates cellular ABA uptake. Compared with WT, the ait1/nrt1.2 mutants were less sensitive to exogenously applied ABA during seed germination and/or postgermination growth, whereas overexpression of AIT1/NRT1.2 resulted in ABA hypersensitivity in the same conditions. Interestingly, the inflorescence stems of ait1/ nrt1.2 had a lower surface temperature than those of the WT because of excess water loss from open stomata. We detected promoter activities of AIT1/NRT1.2 around vascular tissues in inflorescence stems, leaves, and roots. These data suggest that the function of AIT1/NRT1.2 as an ABA importer at the site of ABA biosynthesis is important for the regulation of stomatal aperture in inflorescence stems.guard cells | liquid chromatography-tandem mass spectrometry | water stress

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Long-distance signalling of abscisic acid (ABA): the factors regulating the intensity of the ABA signal

Cited by 256 publications

References 26 publications

Euglena gracilis(Euglenophyceae) produces abscisic acid and cytokinins and responds to their exogenous application singly and in combination with other growth regulators

Euglena gracilis(Euglenophyceae) produces abscisic acid and cytokinins and responds to their exogenous application singly and in combination with other growth regulators

Environmental Nitrate Stimulates Abscisic Acid Accumulation in Arabidopsis Root Tips by Releasing It from Inactive Stores

Identification of an abscisic acid transporter by functional screening using the receptor complex as a sensor

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