Long-Lasting Chemiluminescence-Based POCT for Portable and Visual Pathogenic Detection and In Situ Inactivation

Abstract

Bacterial infections seriously threaten human health and also bring huge financial burden. It is critical to construct multifunctional platforms for effectively inactivating bacteria right after point-of-care testing (POCT). Chemiluminescence (CL) bioassays are considered as powerful candidates for POCT as they are free from using an excitation light source, while the flash-type emission limits their further application. Herein, a CL system with long, persistent, and intensive intensity was constructed based on the peroxidase-like property of 4-mercaptophenylboronic acid (MPBA)-functionalized CuSe nanoprobes (CuSeNPs@MPBA), which improved the detection accuracy and sensitivity. By further integrating a smartphone as an analyzer, quantitative POCT of bacteria was realized with high sensitivity. The limit of detection was as low as 1.25 and 1.01 cfu mL–1 for Staphylococcus aureus and Escherichia coli detection, respectively. Specifically, bacteria can be eliminated with high efficiency due to excellent photothermal property of CuSeNPs@MPBA. The developed multifunctional platform also has advantages of simple operation with low cost, suggesting its high potential for use in food safety, environment monitoring, and clinical applications.

Chemiluminescence Sensor for miRNA-21 Detection Based on CRISPR-Cas12a and Cation Exchange Reaction

“…20 Our group proposed cation exchange-based CL amplification (CXCLAmp) for miRNA detection. 21 On the other hand, the visualization of CL makes it possible to develop a portable biosensor, 22,23 which is in great demand for disease diagnosis and personal health monitoring.…”

Highly Stable Fe/Co-TPY-MIL-88(NH2) Metal–Organic Framework (MOF) in Enzymatic Cascade Reactions for Chemiluminescence-Based Detection of Extracellular Vesicles

“…More interestingly, we found that CL production at this pH could last for at least 1 min (Figure c). In contrast, the no-TPY MOF, Fe/Co-MIL-88­(NH 2 ), only produced a burst of CL that decades rapidly within 20 s. The high stability of the TPY MOF, as well as the limited diffusion of the catalytic center and confined reactants within the porous MOF structures , could both contribute to the strong and long-lasting luminescence emission, which is favorable for accurate and sensitive CL analysis. , The optimal luminol concentration in the CL generation reaction was determined to be 0.032 mM (Figure S8b).…”

Nanozyme: A new Approach for Anti-microbial Infections

“…Materials with high photothermal conversion efficiency usually have complex microstructure and high light absorption capacity. [42] Taking advantage of the good photothermal properties of the nanomaterials, Chen et al [43] designed 4-mercaptophenylboronic acid (MPBA)-functionalized CuSe nanoprobes (CuSeNPs@MPBA) based on chemiluminescence (CL) for the construction of a multifunctional platform for the effective inactivation of bacteria. The nanozyme have peroxidase-like activity to catalyze the conversion of flash CL to luminescent CL, improving the stability and accuracy of the assay, as well as excellent and stable photothermal performance, with outstanding photothermal conversion efficiency (37%) that can significantly increase the temperature under NIR irradiation to cause inactivation of Escherichia coli (99.2%) and Staphylococcus aureus (99.8%).…”