Long non-coding RNA: a new player in cancer

Zhang, Hua; Chen, Zhenhua; Wang, Xinxin; Huang, Zunnan; He, Zhiwei; Chen, Yue‐Qin

doi:10.1186/1756-8722-6-37

Cited by 382 publications

(323 citation statements)

References 76 publications

(82 reference statements)

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“…lncRNAs have been implicated in a large number of cellular processes, such as proliferation, cell cycle progression, growth, and apoptosis (Mercer et al, 2009). In the last 10 years, an increasing number of studies have reported that lncRNA expression is obviously dysregulated in various cancers and that these lncRNAs play critical roles in tumor development, progression, and metastasis (Gibb et al, 2011;Zhang et al, 2013a). In human osteosarcoma, Sun et al (2015b) demonstrated that the lncRNA HULC was significantly upregulated in osteosarcoma tissues and that the overexpression of HULC was correlated with advanced clinical stage and distant metastasis.…”

Section: Introductionmentioning

confidence: 99%

Increased expression of the lncRNA BANCR and its prognostic significance in human osteosarcoma

Zq¹,

R-B²,

Dm³

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Long noncoding RNAs (lncRNAs) play important roles in the formation and progression of many types of human malignancies. The aim of our study was to investigate the expression and biological functions of the lncRNA BRAF-activated noncoding RNA (BANCR) in human osteosarcoma. BANCR expression was quantified by real-time PCR in human osteosarcoma cell lines and tissues. We analyzed the association between BANCR levels and clinicopathological factors and patient prognosis. MTT, flow cytometric, and transwell invasion assays were performed to observe the effects of BANCR on MG-63 cell biological behaviors. BANCR overexpression was observed in osteosarcoma cell lines and clinical specimens. Increased BANCR expression was significantly associated with large tumor size, positive distant metastasis, and advanced clinical stage. High BANCR expression in osteosarcoma was an independent predictor of poor survival. Downregulation of BANCR inhibited MG-63 cell proliferation and invasion and promoted cell apoptosis in vitro. These findings suggested that BANCR may act as a tumor promoter in osteosarcoma and could serve as a potential therapeutic target for this disease.

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Section: Introductionmentioning

confidence: 99%

Increased expression of the lncRNA BANCR and its prognostic significance in human osteosarcoma

Zq¹,

R-B²,

Dm³

et al. 2016

Genet. Mol. Res.

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“…Recent years, lncRNAs have been found to be associated with wide range of biological regulatory functions (31). Many studies have reported that lncRNAs participated in pathogenesis of cancers, epigenome, levels of transcription and post-transcription (32)(33)(34). To date, only a few studies have reported the expression profiles of lncRNA in GC by microarray or sequencing, and with small sample size (35).…”

Section: Discussionmentioning

confidence: 99%

Integrated analysis of long non-coding RNA competing interactions reveals the potential role in progression of human gastric cancer

Liang

Yao

et al. 2016

International Journal of Oncology

112

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Abstract. Abnormal expression of long non-coding RNAs (lncRNAs) have been shown to play an important role in tumor biology. The Cancer Genome Atlas (TCGA) platform is a large sample sequencing database of lncRNAs, and further analysis of the associations between these data and patients' clinical related information can provide new approaches to find the functions of lncRNA. In the present study, 361 RNA sequencing profiles of gastric cancer (GC) patients were selected from TCGA. Then, we constructed the lncRNA-miRNA-mRNA competitive endogenous RNA (ceRNA) network of GC. There were 25 GC specific lncRNAs (fold change >2, p<0.05) identified, 19 of them were included in ceRNA network. Subsequently, we selected these 19 key lncRNAs and analyzed the correlations with clinical features and overall survival, 14 of them were discriminatively expressed with tumor size, tumor grade, TNM stage and lymphatic metastasis (p<0.05). In addition, eight lncRNAs (RPLP0P2, FOXD2-AS1, H19, TINCR, SLC26A4-AS1, SMIM10L2A, SMIM10L2B and SNORD116-4) were found to be significantly associated with overall survival (log-rank p<0.05). Finally, two key lncRNAs HOTAIR and UCA1 were selected for validation of their expression levels in 82 newly diagnosed GC patients by qRT-PCR. Results showed that the fold changes between TCGA and qRT-PCR were 100% in agreement. In addition, we also found that HOTAIR was significantly correlated with tumor size and lymphatic metastasis (p<0.05), and UCA1 was significantly correlated with tumor size, TNM stage and lymphatic metastasis (p<0.05).The clinical relevance of the two lncRNAs and the bioinformatics analysis results were almost the same. Overall, our study showed the GC specific lncRNAs expression patterns and a ceRNA network in GC. Clinical features related to GC specific lncRNAs also suggested these lncRNAs are worthwhile for further study as novel candidate biomarkers for the clinical diagnosis of GC and potential indicators for prognosis. IntroductionNoncoding RNAs (ncRNAs) are transcripts that have no ability of coding proteins, which widely exit in high eukaryotics. According to their characteristics, ncRNAs can be divided into several subtypes including transfer RNA, small nucleolar RNA (snoRNA), ribosomal RNA (rRNA), microRNA (miRNA) and long non-coding RNA (lncRNA). The amount of the ncRNAs transcripts is >98% of the whole genome transcripts and have been suggested to represent transcriptional noise (1). However, more and more evidence indicates that transcriptional output of genome is far more complex than predicted, and suggests new paradigms of ncRNA regulation (2).Recent studies suggest that the ncRNAs may play important biological roles in transcriptional regulation, cellular development, formation of chromosome and RNA modification (3). Based on the transcript size, ncRNAs are grouped into small ncRNAs (<200 bp) and long ncRNAs (>200 bp, up to 100 kb). lncRNA is the functional end-product, and the level of lncRNA expression correlates directly with the level of the active molecule. Thus, ...

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“…A small number of studies suggest that lncRNAs derived from multiple tumors are overexpressed or underexpressed, influencing cancer growth, survival and migration or invasion (26)(27)(28). Furthermore, lncRNAs have been shown to be dysregulated in various types of cancer and several lncRNAs have been functionally associated with cancer cell differentiation (14,29,30).…”

Section: Discussionmentioning

confidence: 99%

Overexpression of lncRNA NEAT1 mitigates multidrug resistance by inhibiting ABCG2 in leukemia

et al. 2016

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Abstract. Leukemia is a heterogeneous clonal disorder in which early hematopoietic cells fail to differentiate and do not undergo programmed cell death or apoptosis. Less than one-third of adult patients with leukemia are managed using current therapies due to the emergence of multidrug resistance (MDR), emphasizing the need for newer and more robust approaches. Recent reports have suggested that long non-coding RNAs (lncRNAs) contribute to selective gene expression and, hence, could be manipulated effectively to halt the progression of cancer. However, little is known regarding the role of lncRNA in leukemia. Nuclear paraspeckle assembly transcript 1 (NEAT1) is a nuclear-restricted lncRNA involved in the pathogenesis of certain types of cancer. Deregulated expression of NEAT1 has been reported in a number of human malignancies, including leukemia and other solid tumors. The present study aimed to characterize the role of NEAT1 in the regulation of MDR in leukemia. Using reverse transcription-quantitative polymerase chain reaction, it was demonstrated that NEAT1 messenger RNA (mRNA) expression levels were significantly downregulated in leukemia patient samples compared with those from healthy donors. Furthermore, NEAT1 mRNA expression was repressed in a number of leukemia cell lines, including K562, THP-1, HL-60 and Jurkat cells, compared with peripheral white blood control cells, consistent with the expression observed in patients with leukemia. In addition, the transfection of a NEAT1 overexpression plasmid into K562 and THP-1 leukemia cell lines alleviated MDR induced by cytotoxic agents, such as Alisertib and Bortezomib, through inhibition of ATP-binding cassette G2. Although more robust studies are warranted, the current findings provide the basis for the use of NEAT1 as a novel promising target in the treatment of leukemia.

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Long non-coding RNA: a new player in cancer

Cited by 382 publications

References 76 publications

Increased expression of the lncRNA BANCR and its prognostic significance in human osteosarcoma

Increased expression of the lncRNA BANCR and its prognostic significance in human osteosarcoma

Integrated analysis of long non-coding RNA competing interactions reveals the potential role in progression of human gastric cancer

Overexpression of lncRNA NEAT1 mitigates multidrug resistance by inhibiting ABCG2 in leukemia

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