Long non-coding RNA cancer susceptibility candidate 2 regulates the function of human fibroblast-like synoviocytes via the microRNA-18a-5p/B-cell translocation gene 3 signaling axis in rheumatoid arthritis

Ye, Zhiqin; Lü, Wei; Yin, Xietian; Li, Huiling; Qin, Guifu; Li, Siqi; Peng, Tingting; Liu, Bo; Zhao, Shusen; Zhuo, Qin

doi:10.1080/21655979.2021.2022075

Cited by 7 publications

(5 citation statements)

References 28 publications

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“…LncRNA CASC2 is found to be dysregulated in several diseases characterized by inflammation and organ injury. 10 , 16 , 17 , 18 For instance, lncRNA CASC2 is upregulated in the pancreatic tissues of acute pancreatitis patients. 18 Meanwhile, lncRNA CASC2 is insufficiently expressed in the serum of rheumatoid arthritis patients.…”

Section: Discussionmentioning

confidence: 99%

“… 18 Meanwhile, lncRNA CASC2 is insufficiently expressed in the serum of rheumatoid arthritis patients. 16 At the same time, lncRNA CASC2 is diminished in the serum of septic AKI patients. 10 Moreover, a study reveals that lncRNA CASC2 is deficiently expressed in septic ALI mice.…”

Section: Discussionmentioning

confidence: 99%

“… 10,16–18 For instance, lncRNA CASC2 is upregulated in the pancreatic tissues of acute pancreatitis patients 18 . Meanwhile, lncRNA CASC2 is insufficiently expressed in the serum of rheumatoid arthritis patients 16 . At the same time, lncRNA CASC2 is diminished in the serum of septic AKI patients.…”

Section: Discussionmentioning

confidence: 99%

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Potential of circulating lncRNA CASC2 as a biomarker in reflecting the inflammatory cytokines, multi‐organ dysfunction, disease severity, and mortality in sepsis patients

Wang

Zhao

Wei

et al. 2022

Clinical Laboratory Analysis

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Background: Long noncoding RNA (lncRNA) cancer susceptibility candidate gene 2 (CASC2) inhibits inflammation and multi-organ dysfunction in various ways. The present study was intended to explore the potency of blood lncRNA CASC2 as a biomarker for sepsis management.Methods: Totally, 184 sepsis patients and 30 healthy controls were enrolled. The reverse transcription-quantitative polymerase chain reaction was used to detect lncRNA CASC2 expression in peripheral blood mononuclear cell samples from the subjects. Mortality during 28 days was recorded in sepsis patients. Results: LncRNA CASC2 was decreased in sepsis patients [median (interquartile range [IQR]): 0.473 (0.241-0.773)] by comparison to healthy controls [median (IQR): 1.019 (0.676-1.685)] (p < 0.001). In sepsis patients, lncRNA CASC2 was negatively correlated with Acute Physiology and Chronic Health Evaluation II (APACHE II) (p = 0.001),Sequential Organ Failure Assessment (SOFA) (p < 0.001), SOFA-respiratory system (p = 0.010), SOFA-coagulation (p = 0.020), SOFA-liver (p = 0.019), and SOFA-renal (p = 0.010) scores, but was not related to SOFA-nervous (p = 0.466) and SOFA-cardio vascular system (p = 0.059) scores. Additionally, lncRNA CASC2 was negatively related to tumor necrosis factorα (p = 0.024), interleukin (IL)-1β (p = 0.013), and IL-17A (p = 0.002), but was not linked to IL-6 (p = 0.112) or IL-10 (p = 0.074). Furthermore, lncRNA CASC2 was lower in sepsis deaths [median (IQR): 0.286 (0.166-0.475)] than in survivors [median (IQR): 0.534 (0.296-0.811)] (p < 0.001). Simultaneously, Kaplan-Meier (KM) curve analysis also observed that lncRNA CASC2 was inversely related to accumulating mortality in sepsis patients (p = 0.003). While lncRNA CASC2 could independently predict lower mortality risk. Conclusion:Circulating lncRNA CASC2 inadequacy indicates the release of inflammatory cytokines, severe multi-organ injuries, and increased mortality in sepsis patients.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Potential of circulating lncRNA CASC2 as a biomarker in reflecting the inflammatory cytokines, multi‐organ dysfunction, disease severity, and mortality in sepsis patients

Wang

Zhao

Wei

et al. 2022

Clinical Laboratory Analysis

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“…ab178013) in line with the corresponding instructions provided with ELISA kits (Abcam). 26 expression triggered by LPS were offset by protopine treatment (Figure 1D). Moreover, the enhanced cell apoptosis index mediated by LPS (7%-25%: Ctrl to 5-μg/mL LPS) and relieved by protopine treatment (25%-8%: 0-20-μM protopine) was detected by flow cytometry (Figure 1E).…”

Section: Enzyme-linked Immunosorbent Serological Assay (Elisa)mentioning

confidence: 97%

Protopine alleviates lipopolysaccharide-triggered intestinal epithelial cell injury through retarding the NLRP3 and NF-κB signaling pathways to reduce inflammation and oxidative stress

Ouyang

et al. 2022

Allergol Immunopathol

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Background: Inflammatory bowel disease (IBD) is a common chronic intestinal disease. Protopine isolated from different plants has been investigated to understand its special functions on varied diseases. However, the regulatory effects of protopine on the progression of IBD remain unclear. Our study is aimed to explore the effects of protopine on the progression of IBD and its underlying regulatory mechanism of action. Methods: The cell viability was assessed through MTT colorimetric assay. The protein expressions of genes were examined by Western blot analysis. The cell apoptosis and reactive oxygen species level were measured using flow cytometry. The levels of inflammation and oxidative stress-related proteins were tested through enzyme-linked-immunosorbent serologic assay. The intracellular Ca2+ concentration and mitochondrial membrane potential were measured through immunofluorescence assay. Results: First, different concentrations of lipopolysaccharide (LPS) were treated with NCM460 cells to establish IBD cell model, and 5-μg/mL LPS was chosen for followed experiments. In this study, we discovered that protopine relieved the LPS-induced inhibited intestinal epithelial cell viability and enhanced cell apoptosis. Moreover, protopine attenuated LPS-stimulated inflammation activation and oxidative stress. Further experiments illustrated that the increased intracellular Ca2+ concentration and decreased mitochondrial membrane potential stimulated by LPS were reversed by protopine treatment. Finally, through Western blot analysis, it was demonstrated that protopine retarded the activated NLR family pyrin domain containing 3 (NLRP3) and nuclear factor kappa B (NF-κB) signaling pathways mediated by LPS. Conclusion: Protopine alleviated LPS-triggered intestinal epithelial cell injury by inhibiting NLRP3 and NF-κB signaling pathways to reduce inflammation and oxidative stress. This discovery may provide a useful drug for treating IBD.

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“…addressed that via the modulation of the TLR4/miR-6089 axis, lncRNA HIX003209 evoked an inflammatory response in RA [ 44 ]. Except that, the signalling axis of LINC01197/miR-150/THBS2 [ 39 ] and lncRNA CASC2/miR-18a-5p/BTG3 [ 56 ] have been elucidated to intensify inflammation and diminish apoptosis of RA-FLS.…”

Section: Regulatory Roles Of Cernas In Ramentioning

confidence: 99%

Long noncoding RNA/circular RNA regulates competitive endogenous RNA networks in rheumatoid arthritis: molecular mechanisms and traditional Chinese medicine therapeutic significances

et al. 2023

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Rheumatoid arthritis (RA) is a systemic and autoimmune disease that is mainly featured abnormal fibroblast-like synoviocyte (FLS) proliferation and inflammatory cell infiltration. Abnormal expression or function of long noncoding RNAs (lncRNAs) and circular RNAs (circRNAs) are closely related to human diseases, including RA. There has been increasing evidence showing that in the competitive endogenous RNA (ceRNA) networks, both lncRNA and circRNA are vital in the biological functions of cells. Nevertheless, the exact mechanism of ceRNA in RA remains to be investigated. Herein, we summarized the molecular potencies of lncRNA/circRNA-mediated ceRNA networks in RA, with emphasis on the phenotypic regulation of ceRNA in the progression of RA, including regulation of proliferation, invasion, inflammation and apoptosis, as well as the role of ceRNA in traditional Chinese medicine (TCM) in the treatment of RA. In addition, we also discussed the future direction and potential clinical value of ceRNA in the treatment of RA, which may provide potential reference value for clinical trials of TCM therapy for the treatment of RA. Key messages Long noncoding RNA/circular RNA can work as the competitive endogenous RNA sponge and participate in the pathogenesis of rheumatoid arthritis. Traditional Chinese medicine and its agents have shown potential roles in the prevention and treatment of rheumatoid arthritis via competitive endogenous RNA.

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Long non-coding RNA cancer susceptibility candidate 2 regulates the function of human fibroblast-like synoviocytes via the microRNA-18a-5p/B-cell translocation gene 3 signaling axis in rheumatoid arthritis

Cited by 7 publications

References 28 publications

Potential of circulating lncRNA CASC2 as a biomarker in reflecting the inflammatory cytokines, multi‐organ dysfunction, disease severity, and mortality in sepsis patients

Potential of circulating lncRNA CASC2 as a biomarker in reflecting the inflammatory cytokines, multi‐organ dysfunction, disease severity, and mortality in sepsis patients

Protopine alleviates lipopolysaccharide-triggered intestinal epithelial cell injury through retarding the NLRP3 and NF-κB signaling pathways to reduce inflammation and oxidative stress

Long noncoding RNA/circular RNA regulates competitive endogenous RNA networks in rheumatoid arthritis: molecular mechanisms and traditional Chinese medicine therapeutic significances

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