Long non-coding RNA ENST00462717 suppresses the proliferation, survival, and migration by inhibiting MDM2/MAPK pathway in glioma

Wang, Aiqin; Meng, Mingzhu; Kong, Lina

doi:10.1016/j.bbrc.2017.02.015

Cited by 19 publications

(21 citation statements)

References 20 publications

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“…MEG3 suppresses glioma cell proliferation, migration and invasion by sponging miR-19a [ 27 ]. ENST00462717 suppresses proliferation, survival, and migration by partially regulating the MDM2/MAPK pathway in glioma [ 28 ]. PTCSC3 suppresses proliferation and induces apoptosis in U87 and U251 cells by targeting LRP6 to suppress the Wnt/β-catenin signalling pathway [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

The long non-coding RNA SNHG14 inhibits cell proliferation and invasion and promotes apoptosis by sponging miR-92a-3p in glioma

et al. 2018

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Malignant glioma is one of the most common types of primary brain tumours. Long non-coding RNAs (lncRNAs) have recently emerged as a new class of therapeutic targets for many cancers. In this study, we aimed to explore the functional involvement of small nucleolar RNA host gene 14 (SNHG14) and its potential regulatory mechanism in glioma progression. SNHG14 was found to be downregulated in human glioma tissues and cell lines. SNHG14 significantly inhibited cell viability, reduced cell invasion, and induced apoptosis in glioma cell lines. Furthermore, a correlation analysis demonstrated that there was a negative correlation between SNHG14 expression and miR-92a-3p expression. Bioinformatics prediction and luciferase reporter assays demonstrated that miR-92a-3p could directly bind to SNHG14. miR-92a-3p was significantly upregulated in glioma and acted as an oncogene in glioma cells by inhibiting Bim. Moreover, mechanistic investigations showed that miR-92a-3p could reverse the tumour suppressive effects induced by SNHG14 in glioma, indicating that SNHG14 may act as an endogenous sponge that competes for binding to miR-92a-3p. Our results suggest that SNHG14 and miR-92a-3p may be promising molecular targets for glioma therapy.

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Section: Discussionmentioning

confidence: 99%

The long non-coding RNA SNHG14 inhibits cell proliferation and invasion and promotes apoptosis by sponging miR-92a-3p in glioma

et al. 2018

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“…In vitro studies have shown its suppressive effect on glioma cell proliferation, survival and migration, which is possibly due to its negative effect on MDM2 expression. In addition, it has a regulatory role on MAPK pathway through decreas ing the phosphorylation of p38, ERK1/ 2, and JNK [47].…”

Section: Lnc00462717mentioning

confidence: 99%

Long Non-coding RNAs as Regulators of the Mitogen-activated Protein Kinase (MAPK) Pathway in Cancer

Tasharrofi¹,

Ghafouri‐Fard²

2018

Klin Onkol

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Considering the role of this pathway in the pathogenesis of several cancers, alterations in lncRNA expression lead to changes in MAPK pathway resulting in inhibition of apoptosis and induction of cell proliferation and migration. Moreover, some lncRNAs participate in cross-talk between MAPK and other cancer-related pathways, such as PI3K/Akt pathway through regulation of certain shared proteins between these pathways. Based on the availability of certain anticancer drugs that modulate this pathway, identification of lncRNAs that affect this pathway would help in establishment of effective therapies.Key words: RNA - long noncoding - mitogen-activated protein kinases - signal transduction.

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“…The amplification and overexpression of MDM2 are present in human malignant gliomas and cell lines, independent of p53 status [36,37], and high MDM2 expression is correlated with poor prognosis of glioma patients [38]. It has also been shown that miR-610 [25] and long non-coding RNA ENST00462717 [26] inhibit glioma cell proliferation by targeting MDM2, which are in agreement with our findings and collectively indicate that MDM2 plays a vital role in maintaining or even promoting glioma cell proliferation. It is likely that MDM2-regulated cell cycle progression is associated with its function in glioma cell proliferation [39,40].…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-585 inhibits human glioma cell proliferation by directly targeting MDM2

et al. 2020

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Background MicroRNAs (miRNAs) are important regulators for cancer cell proliferation. miR-585 has been shown to inhibit the proliferation of several types of cancer, however, little is known about its role in human glioma cells. Methods miR-585 levels in human glioma clinical samples and cell lines were examined by quantitative real-time PCR (qRT-PCR) analysis. Cell proliferation was measured by Cell Counting Kit-8 (CCK-8) and EdU incorporation assays in vitro. For in vivo investigations, U251 cells were intracranially inoculated in BALB/c nude mice and xenografted tumors were visualized by magnetic resonance imaging (MRI). Results miR-585 expression is downregulated in human glioma tissues and cell lines compared with non-cancerous counterparts. Additionally, miR-585 overexpression inhibits and its knockdown promotes human glioma cell proliferation in vitro. Moreover, miR-585 overexpression also inhibits the growth of glioma xenografts in vivo, suggesting that miR-585 may act as a tumor suppressor to inhibit the proliferation of human glioma. Furthermore, miR-585 directly targets and decreases the expression of oncoprotein murine double minute 2 (MDM2). More importantly, the restoration of MDM2 via enforced overexpression markedly rescues miR-585 inhibitory effect on human glioma cell proliferation, thus demonstrating that targeting MDM2 is a critical mechanism by which miR-585 inhibits human glioma cell proliferation. Conclusions Our study unveils the anti-proliferative role of miR-585 in human glioma cells, and also implicates its potential application in clinical therapy.

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Long non-coding RNA ENST00462717 suppresses the proliferation, survival, and migration by inhibiting MDM2/MAPK pathway in glioma

Cited by 19 publications

References 20 publications

The long non-coding RNA SNHG14 inhibits cell proliferation and invasion and promotes apoptosis by sponging miR-92a-3p in glioma

The long non-coding RNA SNHG14 inhibits cell proliferation and invasion and promotes apoptosis by sponging miR-92a-3p in glioma

Long Non-coding RNAs as Regulators of the Mitogen-activated Protein Kinase (MAPK) Pathway in Cancer

MicroRNA-585 inhibits human glioma cell proliferation by directly targeting MDM2

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