Long Non-coding RNA Expression Profile and Functional Analysis in Children With Acute Fulminant Myocarditis

Liu, Qingqing; Kong, Yaru; Han, Bo; Jiang, Diandong; Jia, Honglei; Zhang, Li

doi:10.3389/fped.2019.00283

Cited by 14 publications

(15 citation statements)

References 33 publications

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“…Among them, 1645 lncRNAs were up-regulated and 1456 lncRNAs were down-regulated. Further bioinformatic analysis showed that T cell activation, T cell receptor complex, negative regulation of complement activation, immune response and T-helper 17 cell differentiation process may participate in the progress of VMC (Liu Q. et al, 2019 ). In addition, Zhang et al recently found that lncRNA Ak085865 can promote the expression of anti-inflammatory M2 macrophages, reduce the number of proinflammatory M1 macrophages.…”

Section: The Role Of Lncrnas In Vmcmentioning

confidence: 99%

The Role of Non-coding RNAs in Viral Myocarditis

Zhang

Xiong

Zeng

et al. 2020

Front. Cell. Infect. Microbiol.

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Viral myocarditis (VMC) is a disease characterized as myocardial parenchyma or interstitium inflammation caused by virus infection, especially Coxsackievirus B3 (CVB3) infection, which has no accurate non-invasive examination for diagnosis and specific drugs for treatment. The mechanism of CVB3-induced VMC may be related to direct myocardial damage of virus infection and extensive damage of abnormal immune response after infection. Non-coding RNA (ncRNA) refers to RNA that is not translated into protein and plays a vital role in many biological processes. There is expanding evidence to reveal that ncRNAs regulate the occurrence and development of VMC, which may provide new treatment or diagnosis targets. In this review, we mainly demonstrate an overview of the potential role of ncRNAs in the pathogenesis, diagnosis and treatment of CVB3-induced VMC.

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Section: The Role Of Lncrnas In Vmcmentioning

confidence: 99%

The Role of Non-coding RNAs in Viral Myocarditis

Zhang

Xiong

Zeng

et al. 2020

Front. Cell. Infect. Microbiol.

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“…Bioinformatics analysis of lncRNAs in leukocyte samples from acute fulminant myocarditis patients indicated that dysregulated miRNAs and lncRNAs might participate in the process of T cell activation, including T-helper 17 (Th17) cell differentiation [ 109 ]. Both miR-21 and miR-146b were upregulated in a murine model of viral myocarditis induced by CVB3, and they were demonstrated to modulate Th17 differentiation [ 34 ].…”

Section: Modulation Of Immune Cell Function By Ncrnas During Ev Imentioning

confidence: 99%

Essential Role of Non-Coding RNAs in Enterovirus Infection: From Basic Mechanisms to Clinical Prospects

Zhu

Chen

Zhang

et al. 2021

IJMS

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Enteroviruses (EVs) are common RNA viruses that can cause various types of human diseases and conditions such as hand, foot, and mouth disease (HFMD), myocarditis, meningitis, sepsis, and respiratory disorders. Although EV infections in most patients are generally mild and self-limiting, a small number of young children can develop serious complications such as encephalitis, acute flaccid paralysis, myocarditis, and cardiorespiratory failure, resulting in fatalities. Established evidence has suggested that certain non-coding RNAs (ncRNAs) such as microRNAs (miRNAs), long ncRNAs (lncRNAs), and circular RNAs (circRNAs) are involved in the occurrence and progression of many human diseases. Recently, the involvement of ncRNAs in the course of EV infection has been reported. Herein, the authors focus on recent advances in the understanding of ncRNAs in EV infection from basic viral pathogenesis to clinical prospects, providing a reference basis and new ideas for disease prevention and research directions.

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“…They modulate gene expression at different levels, including chromosome reconstruction, transcription, and post-transcription [9]. LncRNAs play various critical biological functions such as acting as signaling, decoy, guide, and scaffold molecules [10]. For instance, they participate in the modulation of the occurrence and development of various cardiovascular diseases [11].…”

Section: Ivyspringmentioning

confidence: 99%

“…The Mouse cardiomyocytes MCM cells were cultured in Dulbecco's modified eagle medium supplemented with 10% fetal bovine serum, 1% streptomycin, and penicillin and incubated at 37°C and 5% CO2. When the degree of cell fusion reached 70-80%, the old medium was we replaced with fresh complete culture medium containing 1 µg/ml lipopolysaccharides (LPS) [10,25] and incubated for another 24 hours. The cells were then divided into six groups as follows (3 samples in each group): (a) the control group, in which the cells received completed culture media without LPS.…”

Section: Cell Harvestingmentioning

confidence: 99%

Down-regulation of Xist and Mir-7a-5p improves LPS-induced myocardial injury

Liang¹,

Jin²,

Lin³

et al. 2020

Int. J. Med. Sci.

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Background: X-inactive specific transcript (Xist) is a lncRNA, which plays a significant role in X-chromosome inactivation, regulates cell proliferation in tumor cells, and inhibits apoptosis in acute myocardial infarction. On the other hand, miR-7a-5p is involved in cardiomyocytes injury in myocardial ischemia/reperfusion. However, their roles in LPS-induced damage remain unclear. Objectives: This study aimed at using siRNA transfection and lentivirus infection to regulate the expression of xist and miR-7a-5p, and to evaluate their effects on LPS-induced myocardial damage. Method: Mice cardiomyocytes (MCM) cells were divided into six groups, namely the control group, the LPS group, the LPS + lncRNAgroup, the LPS + lncRNA + group, the LPS + miRNAgroup, and the LPS + miRNA + group. Quantitative real-time PCR (qRT-PCR) was performed to assay for the RNA expressions of xist, miR-7a-5p, peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α), and recombinant mitochondrial transcription factor A (Tfam) in all the groups. The ATP level was determined using the adenosine triphosphate (ATP) assay kit according to the manufacturer's instructions. Flow cytometry was performed to estimate the level of apoptosis and proliferation in cells in each group. Results: The level of xist in the myocardial cells was markedly higher in the LPS group compared with the control group; however, it was reduced in the LPS+ lncRNAgroup. There was no significant difference in the expression of xist among the LPS+miRNA-, LPS+miRNA + , and LPS groups. Moreover, the expression of mir-7a-5p was significantly reduced in myocardial cells in the LPS group, and moderately reduced in the LPS+ miRNAgroup, but remarkably elevated in the LPS+ miRNA + group (P<0.05). The expression of mir-7a-5p was comparably similar in the LPS+ lncRNAgroup, LPS+ lncRNA + group, and LPS groups. Further, the levels of PGC-1a, and Tfam were determined. In the LPS group, the expression of PGC-1α was significantly reduced but elevated in the LPS+lncRNA-and LPS+ miRNAgroups (P<0.05). There was no significant difference in the level of PGC-1α among the LPS, LPS+ lncRNA + , and LPS+ miRNA + groups. The expression of Tfam was markedly reduced in the LPS group (P < 0.05), but elevated after the suppression of xist and mir-7a-5p. The expression of Tfam was not significantly different among the LPS group, LPS+ lncRNA + and LPS+ miRNA + groups. Notably, overexpression of mir-7a-5p had a mild effect on the expression of Tfam in the LPS+ miRNA + group compared with the control group. Besides, ATP expression in the LPS group was markedly reduced, but elevated after the inhibition of xist and mir-7a-5p. Suppressing the expression of xist or mir-7a-5p resulted in reduced cell apoptosis and increased cell proliferation. Conclusions: In this study, we established that down-regulation of xist and mir-7a-5p reduces apoptosis in response to LPS.

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Long Non-coding RNA Expression Profile and Functional Analysis in Children With Acute Fulminant Myocarditis

Cited by 14 publications

References 33 publications

The Role of Non-coding RNAs in Viral Myocarditis

The Role of Non-coding RNAs in Viral Myocarditis

Essential Role of Non-Coding RNAs in Enterovirus Infection: From Basic Mechanisms to Clinical Prospects

Down-regulation of Xist and Mir-7a-5p improves LPS-induced myocardial injury

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