Long non-coding RNA H19 is associated with polycystic ovary syndrome in Chinese women: a preliminary study

Qin, Li; Huang, Chuican; Yan, Xiumin; Wang, Yao; Li, Zhongyi; Wei, Xiangcai

doi:10.1507/endocrj.ej19-0004

Cited by 36 publications

(16 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Circulating H19 has been explored as a diagnostic and prognostic biomarker for other conditions including coronary artery disease [43], multiple myeloma [44], and cancers including bladder and gastric cancer [22,40,41,45,46]. In the reproductive tract, circulating H19 is higher in women with polycystic ovary syndrome (PCOS) compared to controls [47], and H19 has been identified as a potential diagnostic and prognostic marker for epithelial ovarian [48] and cervical cancer [49]. However, a link between circulating H19 and markers of ovarian reserve has not previously been described.…”

Section: Discussionmentioning

confidence: 99%

The relationship between H19 and parameters of ovarian reserve

Xia

Burn

Chen

et al. 2020

Reprod Biol Endocrinol

View full text Add to dashboard Cite

Context: The H19 long noncoding RNA (lncRNA) belongs to a highly conserved, imprinted gene cluster involved in embryonic development and growth control. We previously described a novel mechanism whereby the Antimullerian hormone (Amh) appears to be regulated by H19. However, the relationship between circulating H19 and markers of ovarian reserve including AMH not been investigated. Objective: To determine whether H19 expression is altered in women with decreased ovarian reserve.

show abstract

Section: Discussionmentioning

confidence: 99%

The relationship between H19 and parameters of ovarian reserve

Xia

Burn

Chen

et al. 2020

Reprod Biol Endocrinol

View full text Add to dashboard Cite

show abstract

“…Polycystic ovary syndrome (PCOS) is one of the most common gynecologic endocrine disorders, widely considered as a top cause of infertility [1,2]. Globally, it affects 8-13% of women in the reproductive age bracket [3,4].…”

Section: Introductionmentioning

confidence: 99%

Role of gut microbiota in the development of insulin resistance and the mechanism underlying polycystic ovary syndrome: a review

2020

J Ovarian Res

174

132

View full text Add to dashboard Cite

Polycystic ovary syndrome (PCOS) is a complex endocrine and metabolic disorder. Typically, it is characterized by hirsutism, hyperandrogenism, ovulatory dysfunction, menstrual disorders and infertility. To date, its pathogenesis remains unclear. However, insulin resistance (IR) is considered as the primary pathological basis for its reproductive dysfunction. On the other hand, a condition in which insulin is over-secreted is called hyperinsulinemia. IR/ Hyperinsulinemia is associated with chronic inflammation, hormonal changes, follicular dysplasia, endometrial receptivity changes, and abortion or infertility. Additionally, it increases incidence of complications during pregnancy and has been associated with anxiety, depression, and other psychological disorders. Gut microbiota, the "second genome" acquired by the human body, can promote metabolism, immune response through interaction with the external environment. Gut microbiota dysbiosis can cause IR, which is closely linked to the occurrence of PCOS. This article reviewed recent findings on the roles of gut microbiota in the development of insulin resistance and the mechanism underlying polycystic ovary syndrome.

show abstract

“…In our previous study, we found that lncRNA H19 was highly expressed in peripheral blood of patients with PCOS (15). There are many studies on CTGF in PCOS that confirm that CTGF is highly expressed in PCOS (16), but the reason why is unknown.…”

Section: Discussionmentioning

confidence: 96%

lncRNA H19 acts as a ceRNA to regulate the expression of CTGF by targeting miR-19b in polycystic ovary syndrome

Sun

Yan

Liu

et al. 2020

Braz J Med Biol Res

Self Cite

View full text Add to dashboard Cite

The etiology of polycystic ovary syndrome (PCOS) is complex and the pathogenesis is not fully understood. Some studies have shown that dysregulation of ovarian granulosa cells may be related to abnormal follicles and excessive androgen in women with PCOS. Our team has also confirmed the high expression status of H19 in PCOS patients in the early stage. However, the relationship between H19 and miR-19b in the development of PCOS is still unknown. Therefore, we used bioinformatics to predict the binding sites of human H19 and miR-19b, and of miR-19b and CTGF genes. After the silencing and overexpression of H19, real-time polymerase chain reaction (PCR) was used to detect the expressions of H19, miR-19b, and CTGF. Western blotting was used to detect CTGF protein. Proliferation of KGN cells after H19 silencing was detected by CCK8. Flow cytometry was used to detect the apoptosis of KGN cells after H19 silencing. After the overexpression of H19, it was found that the expression of miR-19b gene decreased and the expression of CTGF increased, whereas silencing of H19 did the opposite. In addition, H19 could promote cell proliferation and decrease cell apoptosis. Finally, luciferase reporter assays showed that the 3 0-end sequences of lncRNA H19 and CTGF contained the binding site of miR-19b. In conclusion, our study indicated that lncRNA H19 acted as a ceRNA to bind to miR-19b via a ''sponge'' to regulate the effect of CTGF on KGN cells, which may play a vital role in PCOS.

show abstract

Long non-coding RNA H19 is associated with polycystic ovary syndrome in Chinese women: a preliminary study

Cited by 36 publications

References 44 publications

The relationship between H19 and parameters of ovarian reserve

The relationship between H19 and parameters of ovarian reserve

Role of gut microbiota in the development of insulin resistance and the mechanism underlying polycystic ovary syndrome: a review

lncRNA H19 acts as a ceRNA to regulate the expression of CTGF by targeting miR-19b in polycystic ovary syndrome

Contact Info

Product

Resources

About