Long Non-Coding RNA KCNQ1OT1 Promotes Progression of Hepatocellular Carcinoma by miR-148a-3p/IGF1R Axis

Xu, Guoping; Zhu, Yungang; Liu, Huijia; Liu, Yingying; Zhang, Xuening

doi:10.1177/1533033820980117

Cited by 17 publications

(10 citation statements)

References 34 publications

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“…However, deletion of its promoter or early transcript termination results in a loss of KCNQ1OT1 and a disruption of imprinting in the CDKN1C/KCNQ1OT1 domain, which can lead to growth-related disorders (ex. Beckwith-Wiedemann syndrome) and cancer, as well as bi-allelic expression of the entire KCNQ1 domain [ 31 ].…”

Section: Basic Characteristics Of Human Chromosome 11p155 and Lncrna Kcnq1ot1 Genementioning

confidence: 99%

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KCNQ1OT1: An Oncogenic Long Noncoding RNA

Cagle

Niture

et al. 2021

Biomolecules

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Long noncoding RNAs (lncRNAs) are transcripts greater than 200 nucleotides that do not code for proteins but regulate gene expression. Recent studies indicate that lncRNAs are involved in the modulation of biological functions in human disease. KCNQ1 Opposite Strand/Antisense Transcript 1 (KCNQ1OT1) encodes a lncRNA from the opposite strand of KCNQ1 in the CDKN1C/KCNQ1OT1 cluster that is reported to play a vital role in the development and progression of cancer. KCNQ1OT1 regulates cancer cell proliferation, cell cycle, migration and invasion, metastasis, glucose metabolism, and immune evasion. The aberrant expression of KCNQ1OT1 in cancer patients is associated with poor prognosis and decreased survival. This review summarizes recent literature related to the biological functions and molecular mechanisms of KCNQ1OT1 in various human cancers, including colorectal, bladder, breast, oral, melanoma, osteosarcoma, lung, glioma, ovarian, liver, acute myeloid leukemia, prostate, and gastric. We also discuss the role of KCNQ1OT1 as a promising diagnostic biomarker and a novel therapeutic target in human cancers.

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Section: Basic Characteristics Of Human Chromosome 11p155 and Lncrna Kcnq1ot1 Genementioning

confidence: 99%

“…In addition, Wang et al found that KCNQ1OT1 promoted GC progression by sponging miR-4319 to upregulate the expression of DNA-damage-regulated autophagy modulator 2 (DRAM2) [ 46 ]. Moreover, KCNQ1OT1 is discussed as a ceRNA for miR-148a-3p and a positive regulator for IGF1R in HCC [ 31 ].…”

Section: Regulatory Mechanisms Of Kcnq1ot1mentioning

confidence: 99%

KCNQ1OT1: An Oncogenic Long Noncoding RNA

Cagle

Niture

et al. 2021

Biomolecules

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“…An increasing number of studies have confirmed that lncRNAs can act as miRNA sponges and thus reduce their regulatory effects on the target mRNAs; for instance, KCNQ1OT1 acts as miR-216b-5p sponge to attenuate its binding ability to ZNF146 and induce upregulation of ZNF146 expression in colorectal cancer cells [ 25 ]. In addition, KCNQ1OT1 can competitively target miR-148a-3p and consequently promote the expression of the miR-148a-3p target IGF1R gene in hepatocellular carcinoma cells [ 26 ]. KCNQ1OT1 acts as a ceRNA of miR-200a to upregulate the miR-200a downstream forkhead box O3 (FOXO3) expression, thus influencing the pathology of cerebral ischemic stroke [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

Screening of lncRNA-miRNA-mRNA Coexpression Regulatory Networks Involved in Acute Traumatic Coagulation Dysfunction Based on CTD, GeneCards, and PharmGKB Databases

Luo

Tan

et al. 2022

Oxidative Medicine and Cellular Longevity

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Competitive endogenous RNA (ceRNA) networks play crucial roles in multiple biological processes and development of diseases. They might serve as diagnostic and prognosis markers as well as therapeutic targets. The purpose of this study was to identify a novel ceRNA network involving KCNQ1OT1, hsa-miR-24-3p, and VWF in acute traumatic coagulopathy (ATC) based on databases search. We searched the CTD, GeneCards, and PharmGKB databases for ATC-related target genes using Coagulopathy as a keyword. Upstream miRNAs and lncRNAs of the candidate target VWF were then explored using the miRWalk, microT, TargetScan, RNA22 and Tarbase, and DIANA-LncBase and Starbase databases, respectively. A KCNQ1OT1-hsa-miR-24-3p-VWF ceRNA network was constructed by R “ggalluvial” package. Interaction between KCNQ1OT1, hsa-miR-24-3p, and VWF was examined, and their expression was quantified in the peripheral blood samples from 30 ATC patients and liver tissues of ATC rat models. Forty-one ATC-related target genes were identified following data retrieval from publicly available databases, of which VWF was selected as the target and used for the subsequent analysis. KCNQ1OT1 and hsa-miR-24-3p were confirmed to be the key upstream regulatory factors of VWF. KCNQ1OT1-hsa-miR-24-3p-VWF coexpression regulatory network was constructed where KCNQ1OT1 competitively bound to hsa-miR-24-3p and attenuated its binding to VWF. Both the liver tissues of ATC rats and peripheral blood samples from ATC patients showed increased hsa-miR-24-3p expression and decreased VWF and KCNQ1OT1 expression. Collectively, we described the KCNQ1OT1-hsa-miR-24-3p-VWF ceRNA network in the development of ATC. We propose a new ceRNA that could help in the diagnosis and treatment of ATC.

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“…KCNQ1OT1 promoted atherosclerosis development by modulating miR-452-3p/HDAC3/ABCA1 pathway (Yu et al 2020 ). Additionally, KCNQ1OT1 exerted tumor-promoting roles in colorectal carcinogenesis (Chen et al 2020b ), non-small-cell lung carcinoma (Dong et al 2019 ), hepatocellular carcinoma (Xu et al 2020 ), bladder cancer (Wang et al 2019a ), glioma (Ding et al 2020 ), osteosarcoma (Wang et al 2019b ), gastric cancer (Wang et al 2020 ) and OC (Liu et al 2020 ). Consistent to research of Liu et al ( 2020 ), we found that KCNQ1OT1 expression was increased in OC tissues and cells.…”

Section: Discussionmentioning

confidence: 99%

LncRNA KCNQ1OT1 promotes the metastasis of ovarian cancer by increasing the methylation of EIF2B5 promoter

Chen

et al. 2022

Mol Med

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Background Long non-coding RNAs (lncRNAs) have emerged as regulators of human malignancies, including ovarian cancer (OC). LncRNA KCNQ1OT1 could promote OC progression, and EIF2B5 was associated with development of several tumors. This project was aimed to explore the role of lncRNA KCNQ1OT1 in OC development, as well as the involving action mechanism. Methods Reverse transcription quantitative polymerase chain reaction (RT-qPCR) or Western blotting was employed to determine the expression levels of KCNQ1OT1 and EIF2B5. OC cell proliferation was evaluated by MTT and colony formation assays, and wound healing and Transwell assays were implemented to monitor cell migration and invasion, respectively. The methylation status of EIF2B5 promoter was examined by MS-PCR, to clarify whether the expression of EIF2B5 was decreased. The binding activity of KCNQ1OT1 to methyltransferases DNMT1, DNMT3A and DNMT3B was determined by dual luciferase reporter assay or RIP assay, to explore the potential of KCNQ1OT1 alters the expression of its downstream gene. ChIP assay was carried out to verify the combination between EIF2B5 promoter and above three methyltransferases. Results Expression of lncRNA KCNQ1OT1 was increased in OC tissues and cells. EIF2B5 expression was downregulated in OC, which was inversely correlated with KCNQ1OT1. Knockdown of KCNQ1OT1 inhibited OC cell proliferation and metastasis. KCNQ1OT1 could downregulate EIF2B5 expression by recruiting DNA methyltransferases into EIF2B5 promoter. Furthermore, interference of EIF2B5 expression rescued KCNQ1OT1 depletion-induced inhibitory impact on OC cell proliferation and metastasis. Conclusion Our findings evidenced that lncRNA KCNQ1OT1 aggravated ovarian cancer metastasis by decreasing EIF2B5 expression level, and provided a novel therapeutic strategy for OC.

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Long Non-Coding RNA KCNQ1OT1 Promotes Progression of Hepatocellular Carcinoma by miR-148a-3p/IGF1R Axis

Cited by 17 publications

References 34 publications

KCNQ1OT1: An Oncogenic Long Noncoding RNA

KCNQ1OT1: An Oncogenic Long Noncoding RNA

Screening of lncRNA-miRNA-mRNA Coexpression Regulatory Networks Involved in Acute Traumatic Coagulation Dysfunction Based on CTD, GeneCards, and PharmGKB Databases

LncRNA KCNQ1OT1 promotes the metastasis of ovarian cancer by increasing the methylation of EIF2B5 promoter

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