Long Non-Coding RNA linc00645 Promotes TGF-β-Induced Epithelial-Mesenchymal Transition by Regulating miR-205-3p-ZEB1 Axis in Glioma

Li, Chenlong; Zheng, Hongshan; Hou, Weiliang; Bao, Hongbo; Xiong, Jinsheng; Che, Wanli; Gu, Yu; Sun, Haiming; Peng, Liang

doi:10.2139/ssrn.3260780

Cited by 17 publications

(20 citation statements)

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“…Tumor cells and matrix components have been reported to participate in the proliferation, progression and recurrence of glioma ( 22 ). Modulating the EMT process is important for reversing the malignant features of glioma and may be beneficial for clinical therapies ( 23 ). The present study demonstrated that KCNQ1OT1 expression was associated with the prognosis of patients with glioma, and regulated the cellular phenotypes of glioma cells by modulating the miR-375/Yes-associated protein (YAP) axis.…”

Section: Introductionmentioning

confidence: 99%

lncRNA KCNQ1OT1 promotes proliferation and invasion of glioma cells by targeting the miR‑375/YAP pathway

et al. 2020

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The long non-coding RNA KCNQ1OT1 is generally recognized as an oncogenic molecule in several human malignant tumors. However, to the best of our knowledge, the role of KCNQ1OT1 in glioma has not been fully investigated. The current study aimed to probe the biological function of KCNQ1OT1 in human glioma cell lines and its mechanisms. The glioma cell lines U251 and U87-MG were used as cell models. Cell proliferation and apoptosis assays were used to measure the effects of different treatments on survival, and reverse transcription-quantitative PCR and western blot-ting were used to investigate the expression profiles of key molecules. Migration and invasion assays were conducted to reveal the biological features of glioma cells. The results indicated that KCNQ1OT1 was upregulated in glioma tissues compared with adjacent tissues, which was associated with poor prognosis. Additionally, knockdown of KCNQ1OT1 in U251 and U87-MG cells inhibited cell proliferation, migration and invasion, but had no effect on apoptosis. The effects of KCNQ1OT1 on migration and invasion were partially attributed to enhanced Yes-associated protein (YAP) expression levels and epithelial-mesenchymal transition (EMT) signaling. Furthermore, microRNA (miR)-375 functioned as a link between KCNQ1OT1 and YAP in regulating cell proliferation. Finally, the KCNQ1OT1/miR-375/YAP axis modulated cell proliferation and cell fate by affecting the modulated YAP-mediated EMT signaling. In conclusion, the KCNQ1OT1/miR-375/YAP axis modulated migration and invasion of glioma cells by affecting EMT signaling; thus, targeting KCNQ1OT1 may represent a promising strategy in glioma therapeutics.

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Section: Introductionmentioning

confidence: 99%

lncRNA KCNQ1OT1 promotes proliferation and invasion of glioma cells by targeting the miR‑375/YAP pathway

et al. 2020

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“…By interacting with miRNA, a lncRNA named linc00645 promotes TGF-B-induced EMT by sequestering miR-205-3p, which normally downregulates ZEB1 expression. Under the circumstance of ZEB1 overabundance, (TGF)-β-induced motility of glioma cells occurs [ 123 ]. Another example of EMT-related lncRNAs is TCON, which targets the Smad/PKCα signaling pathway and simultaneously negatively regulates the progression of the malignant process, thus considered as a GBM oncosuppressor.…”

Section: Ncrnas In Brain Tumor Progressionmentioning

confidence: 99%

Non-Coding RNAs in Brain Tumors, the Contribution of lncRNAs, circRNAs, and snoRNAs to Cancer Development—Their Diagnostic and Therapeutic Potential

Latowska

Grabowska

Zarębska

et al. 2020

IJMS

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Brain tumors are one of the most frightening ailments that afflict human beings worldwide. They are among the most lethal of all adult and pediatric solid tumors. The unique cell-intrinsic and microenvironmental properties of neural tissues are some of the most critical obstacles that researchers face in the diagnosis and treatment of brain tumors. Intensifying the search for potential new molecular markers in order to develop new effective treatments for patients might resolve this issue. Recently, the world of non-coding RNAs (ncRNAs) has become a field of intensive research since the discovery of their essential impact on carcinogenesis. Some of the most promising diagnostic and therapeutic regulatory RNAs are long non-coding RNAs (lncRNAs), circular RNAs (circRNAs), and small nucleolar RNAs (snoRNAs). Many recent reports indicate the important role of these molecules in brain tumor development, as well as their implications in metastasis. In the following review, we summarize the current state of knowledge about regulatory RNAs, namely lncRNA, circRNAs, and snoRNAs, and their impact on the development of brain tumors in children and adults with particular emphasis on malignant primary brain tumors—gliomas and medulloblastomas (MB). We also provide an overview of how these different ncRNAs may act as biomarkers in these tumors and we present their potential clinical implications.

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“…2H, p < 0.001). Given that epithelial-mesenchymal transition (EMT) is one of the major mechanisms for cancer metastasis, we further evaluated the effect of LINC00645 on EMT-related markers [9]. Western blot analysis showed that LINC00645 knockdown could increase the expression of epithelial markers (E-cadherin) and decrease the expression of mesenchymal markers (N-cadherin) ( Fig.…”

Section: Linc00645 Expression Is Up-regulated In Hcc Cell Lines Hcc mentioning

confidence: 99%

“…Long intergenic non-protein coding RNA 645 (LINC00645) was rst annotated as a long intergenic noncoding RNA (lincRNA) on chromosome 14q12. Recent studies have reported that LINC00645 plays an oncogenic role in glioma and it may serve as a prognostic biomarker and a potential therapeutic target for the treatment of glioma in humans [9]. However, the function and mechanism of LINC00645 as ceRNAs in HCC remain unclear.…”

Section: Introductionmentioning

confidence: 99%

MAZ-LINC00645-GP73 Axis Promotes Hepatocellular Carcinoma Proliferation and Metastasis

Liu

Han

et al. 2020

Preprint

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BACKGROUND: Long non-coding RNAs (lncRNA) have been shown to play important roles in the development and progression of hepatocellular carcinoma (HCC). In this report, we examined the role of lncRNA LINC00645 in HCC. MATERIAL AND METHODS: Based on public databases and integrating bioinformatics analyses, the over-expression of LINC00645 in HCC tissues was detected and further validated in a cohort of liver tissues. A series of in vitro and in vivo functional experiments were executed to investigate the role of LINC00645 in the carcinogenesis and development of HCC. Comprehensive transcriptional analysis, chromatin immunoprecipitation (ChIP) assay, dual-luciferase reporter assay and western blot etc. were performed to explore the molecular mechanisms underlying the functions of LINC00645. RESULTS: LINC00645 was significantly upregulated in HCC cell lines and HCC tissues, which was correlated with poor prognosis in HCC patients. LINC00645 knockdown remarkably suppressed tumor growth in vitro and in vivo. Mechanistically, LINC00645 could competitively bind with miR-141-3p to prevent the degradation of its target gene GP73, which acts as a tumor-promoter in HCC. Furthermore, the ChIP assay showed that the transcription factor MAZ could bind to the LINC00645 promoter and increase its transcription. CONCLUSIONS: Collectively, this study demonstrated that LINC00645 plays a critical regulatory role in hepatocellular carcinoma cells and LINC00645 may serve as a potential diagnostic biomarker and therapeutic target of HCC. Thus, targeting MAZ/LINC00645/miR-141-3p/GP73 signaling axis may prevent the progression of HCC.

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Long Non-Coding RNA linc00645 Promotes TGF-β-Induced Epithelial-Mesenchymal Transition by Regulating miR-205-3p-ZEB1 Axis in Glioma

Cited by 17 publications

References 0 publications

lncRNA KCNQ1OT1 promotes proliferation and invasion of glioma cells by targeting the miR‑375/YAP pathway

lncRNA KCNQ1OT1 promotes proliferation and invasion of glioma cells by targeting the miR‑375/YAP pathway

Non-Coding RNAs in Brain Tumors, the Contribution of lncRNAs, circRNAs, and snoRNAs to Cancer Development—Their Diagnostic and Therapeutic Potential

MAZ-LINC00645-GP73 Axis Promotes Hepatocellular Carcinoma Proliferation and Metastasis

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