Long non‑coding RNA lnc‑GNAT1‑1 inhibits gastric cancer cell proliferation and invasion through the Wnt/β‑catenin pathway in Helicobacter�pylori infection

Abstract

The aim of the present study was to investigate the effect of long non‑coding (lnc)‑G protein subunit α transducin 1 (GNAT1)‑1 in gastric cancer cells infected with Helicobacter (H.) pylori. Reverse transcription‑quantitative polymerase chain reaction was used to assess lnc‑GNAT1‑1 expression in normal gastric and gastric cancer cells infected with H. pylori. The overexpression of lnc‑GNAT1‑1 in SGC‑7901 and MNK45 cells was induced by transfection. Migration and invasion assays were performed in transfected gastric cancer cells to evaluate the effect of lnc‑GNAT1‑1. Histological and western blot analyses were used to determine the alterations in the Wnt/β‑catenin signaling pathway protein expression. Cells transfected with lnc‑GNAT1‑1 were used to initiate gastric cancer tumor xenografts to compare tumor growth in mice inoculated with untransfected cells. The results revealed that H. pylori infection significantly downregulated lnc‑GNAT‑1 expression. Lnc‑GNAT1‑1 overexpression inhibited gastric cancer cell migration and invasion. Wnt/β‑catenin pathway protein expression was decreased by lnc‑GNAT1‑1 overexpression. In addition, lnc‑GNAT1‑1 overexpression reduced tumor growth. Thus, lnc‑GNAT1‑1 is downregulated in gastric cancer infected with H. pylori and lnc‑GNAT1‑1 overexpression inhibited gastric cancer growth through the Wnt/β‑catenin signaling pathway.

<p>lncRNA MAFG-AS1 Contributes to Esophageal Squamous-Cell Carcinoma Progression via Regulating miR143/LASP1</p>

“… 7 Studies have recognized that lncRNA, as a multitalented transcriptional and posttranscriptional regulator of key cancer pathways, possesses lots of biological functions involved in many types of cancer progression, 8 such as hepatocellular carcinoma, 9 lung cancer (LC), 10 colorectal cancer (CRC), 11 and gastric cancer. 12 A number of cross-sectional studies have suggested numerous lncRNAs being related to the development of ESCC. It has been reported that NORAD was correlated with tumor size and overall survival in ESCC.…”

Long Non-coding RNA lnc-GNAT1-1 Suppresses Liver Cancer Progression via Modulation of Epithelial–Mesenchymal Transition

“…The underlying mechanism is thought to be related to the regulation of the RKIP-NF-κB-Snail circuit (Ye et al, 2016). Downregulation of lnc-GNAT1-1 was observed in patients with gastric cancer and lower levels of Helicobacter pylori infection and increased expression levels of lnc-GNAT1-1 suppressed gastric cancer growth by inactivating the Wnt/β-catenin pathway (Liu et al, 2018). Against this background, we explored the roles of lnc-GNAT1-1 in liver cancer development and the mechanisms used by lnc-GNAT1-1 to exert modulatory effects on liver cancer progression.…”

“…Previous studies have indicated that lnc-GNAT1-1 plays an anti-tumor role in malignancies of the digestive tract, including colorectal (Ye et al, 2016) and gastric cancers (Liu et al, 2018). Nevertheless, there are no studies of lnc-GNAT1-1 in liver cancer.…”

Chlamydia trachomatis Plasmid Protein pORF5 Up-Regulates ZFAS1 to Promote Host Cell Survival via MAPK/p38 Pathway

“…Meanwhile, growing evidence implies that altered expression of lncRNAs could be closely related to genesis and progression of numerous diseases, especially in cancers and viral infections ( Liu and Ding, 2017 ; Sun et al, 2020 ). Recently, numerous studies have provided evidence for the involvement of lncRNAs in bacterial infections, including Mycobacterium tuberculosis , Helicobacter pylori , Listeria monocytogenes , and so forth ( Liu et al, 2018 ; Li M. et al, 2019 ; Xu et al, 2019 ). However, few studies have evaluated the alternation of lncRNAs in obligate intracellular bacterium C. trachomatis .…”