Long non‐coding RNA long intergenic non‐protein coding RNA 1232 promotes cell proliferation, migration and invasion in bladder cancer via modulating miR‐370‐5p/PIM3 axis

Abstract

Increasing evidences have suggested that long non‐coding RNAs are critical regulators in the progression of tumor growth. Long intergenic non‐protein coding RNA 1232 (LINC01232) was verified as an oncogene in multiple cancers. Nevertheless, its function in bladder cancer (BC) remains to be uncovered. In the current study, we detected LINC01232 expression utilizing quantitative real‐time polymerase chain reaction (RT‐qPCR) and discovered that LINC01232 was overexpressed in BC cell lines versus normal cell line. Besides, the effect of LINC01232 on BC cell behaviors was measured by colony formation, Cell Counting Kit‐8 (CCK‐8), transwell, TdT‐mediated dUTP Nick‐End Labeling and caspase‐3/8 activity assays. Functionally, LINC01232 deficiency suppressed cell proliferation, migration and invasion. Next, miR‐370‐5p was proved to bind with LINC01232 by RNA pull down, RNA‐binding protein immunoprecipitation (RIP) and luciferase reporter assays. Furthermore, PIM3 expression was negatively modulated by miR‐370‐5p and markedly increased in BC cell lines. Moreover, PIM3 silence repressed proliferation, migration and invasion but triggered apoptosis of BC cells. The rescue assays validated that upregulation of PIM3 recovered the effects of LINC01232 silence on the growth of BC cells. To summarize, our study manifested that LINC01232 accelerates BC progression by targeting miR‐370‐5p/PIM3 axis. Targeting LINC01232 might offer novel insight into BC treatment.

hsa_circ_0003596, as a novel oncogene, regulates the malignant behavior of renal cell carcinoma by modulating glycolysis

“…As an example, Sang et al have observed the downregulation of miR-370-5p in breast cancer, and have experimentally confirmed that miR-370-5p overexpression represses proliferation and invasion of cancer cells. Intriguingly, miR-370-5p can obstruct bladder cancer cells to proliferate, migrate, and invade [ 14 ]. Moreover, downregulating miR-370-5p induces proliferative and invasive actions of colorectal carcinoma cells [ 31 ].…”

“…The correct identification of the potential interaction between circRNAs and miRNAs improves the insight into disease pathogenesis, as well as provides a niche for diagnosing and managing the disease [ 11 ]. miR-370-5p has been widely accepted as a tumor suppressor [ 12 – 14 ]. Definitely, the action of miR-370-5p in RCC progression, especially in the field of glycolysis, is uncertain.…”

Prognostic analysis of RAS-related lncRNAs in liver hepatocellular carcinoma

“…Other research indicates that LINC01232 sequesters microRNA (miRNA)-654-3p and consequently promotes hepatoma-derived growth factor expression to contribute to the tumor progression in esophageal squamous cell carcinoma (28). Moreover, LINC01232 has been proven to promote cell proliferation, migration, and invasion by modulating the miR-370-5p-PIM3 axis in bladder cancer (29). Interestingly, AL031985.…”

“…Other research indicates that LINC01232 sequesters microRNA (miRNA)-654-3p and consequently promotes hepatoma-derived growth factor expression to contribute to the tumor progression in esophageal squamous cell carcinoma ( 28 ). Moreover, LINC01232 has been proven to promote cell proliferation, migration, and invasion by modulating the miR-370-5p–PIM3 axis in bladder cancer ( 29 ). Interestingly, AL031985.3 has been included in several lncRNA-based signatures related to ferroptosis, pyroptosis, immune, and autophagy in predicting the outcome, immune cell infiltration, and response to immunotherapy in patients with LIHC ( 30 - 33 ).…”