Long non-coding RNA MSTO2P promotes the proliferation and colony formation in gastric cancer by indirectly regulating miR-335 expression

Han, Li; Zhu, Haijing; Zhou, Yanbing; Wang, Haibo; Niu, Zhaojian; Shen, Yi; Lv, Liang

doi:10.1177/1010428317705506

Cited by 18 publications

(16 citation statements)

References 35 publications

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“…Despite the identification of a number of miRNA targets involved in human tumors, in the majority of cases their mechanisms of effect remain unclear. The expression of miR‐335 has been shown to be aberrant in tumor tissue from several types of cancer, and is associated with cell proliferation and inflammation . The present study found that miR‐335 was downregulated in NSCLC tumor tissues compared with adjacent non‐tumor tissues.…”

Section: Discussionsupporting

confidence: 52%

miR‐335 inhibited cell proliferation of lung cancer cells by target Tra2β

et al. 2017

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Accumulating evidence has suggested that the dysregulation of miRNA is an important factor in the pathogenesis of lung cancer. Here, we demonstrate that miR‐335 expression is reduced in non‐small cell lung cancer (NSCLC) tumors relative to non‐cancerous adjacent tissues, while the expression of Tra2β is increased. In addition, clinical data revealed that the increased Tra2β and decreased miR‐335 expression observed in NSCLC cells was associated with poor patient survival rates. In vitro experimentation showed that the overexpression of miR‐335 inhibited the growth, invasion and migration capabilities of A459 lung cancer cells, by targeting Tra2β. In contrast, inhibition of miR‐335 or overexpression of the Tra2β target gene stimulated the growth, invasion and migratory capabilities of A459 lung cancer cells in vitro. Furthermore, overexpression of miR‐335 or inhibition of Tra2β decreased the phosphorylation of Rb‐S780 and Rb‐AKT. Overall, these findings suggest that the downregulation of miR‐335 in A459 lung cancer cells promoted cell proliferation through upregulation of Tra2β, mediated via activation of the AKT/mTOR signaling pathway, and suggest that miR‐335 may have potential as a novel therapeutic target for NSCLC.

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Section: Discussionsupporting

confidence: 52%

miR‐335 inhibited cell proliferation of lung cancer cells by target Tra2β

et al. 2017

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“…Until now, numerous studies have proved that lncRNAs were highly active in plenty of pathological processes of GC, such as proliferation and metastasis. Among them, some lncRNAs were de ned as protective factors while others were risk factors [25,[41][42][43][44]. Furthermore, several studies have proved that lncRNAs participated in the progression, especially malignant progression of GC through regulating autophagy-related mRNAs [24][25][26][27][28][29]45].…”

Section: Discussionmentioning

confidence: 99%

Prognostic model based on autophagy-related lncRNAs in gastric cancer

Cheng¹,

Cao²,

Chen³

2020

Preprint

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Background: Gastric cancer (GC) is one of the most pravelent cancer in the world. Although increasing studies have indicated that autophagy-related long non-coding RNA (lncRNA) plays an essential role in the occurrence of GC, the prognosis of GC based on autophagy is still deficient.Method: Autophagy-related lncRNAs were obtained by using the correlation test with the autophagy-related gene. Data was downloaded from The Cancer Genome of Atlas stomach adenocarcinoma (TCGA-STAD) dataset. The prognostic autophagy-related lncRNAs significantly correlated with survival of TCGA-STAD dataset were obtained by using Kaplan-Meier and univariate Cox regression analysis. TCGA-STAD dataset was separated into a training set and a testing set randomly. The model was constructed based on the training set through the least absolute shrinkage and selection operator (LASSO) regression. The testing set and TCGA-STAD were used to validate the accuracy of the model. Every patient got a risk score (RS) and patients were separate into high-risk group and low-risk group due to the median RS. The prognostic network was built and the mRNAs in the system were analyzed through Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. The signaling pathways that the differentially expressed genes (DEGs) between two types of risk group mainly participated in were distinguished through Gene Set Enrichment Analysis (GSEA). The individual’s survival rate was predicted through the nomogram.Results: 24 autophagy-related lncRNAs were found strongly associated with the survival of the TCGA-STAD dataset. Among them, 11 lncRNAs were selected to build the risk score model through LASSO regression. The multivariate Cox analysis showed that the RS could be an independent prognosis predictor. The Kaplan-Meier survival analysis and the Receiver Operating Characteristic (ROC) curve indicated the model had an excellent prediction effect. GO, and KEGG analysis revealed that the mRNAs in the prognostic network were mainly involved in the autophagy and ubiquitin-like protein ligase binding. GSEA analysis uncovered that the DEGs in high-risk group partially participated in the ECM receptor interaction and other signaling pathways.Conclusions: Our results indicated that the risk score model based on the autophagy-related lncRNAs performed well in the prediction of prognosis for patients with GC.

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“…In contrast, Xu et al, (2012) found down regulation of miR-335 in gastric cancer, they reported that lymph node metastasis, poor pT stage, poor pN stage, and invasion of lymphatic vessels, was significantly associated with low expression of miR-335, suggesting a metastasis suppressor function of miR-335 in this disease. miR-335 has been demonstrated to play important roles gastrointestinal tract cancer including GBC (Li et al, 2017;Zhang et al, 2018;Feng et al, 2018;Lu et al, 2016;Zhang et al, 2014). However there has been scarcity of studies on GBC showing the prognostic and therapeutic importance of miR-335.…”

Section: Discussionmentioning

confidence: 99%

Low Expression of MicroRNA335-5p Is Associated with Malignant Behavior of Gallbladder Cancer: A Clinicopathological Study

Fatima

Srivastava

Nigam

et al. 2019

Asian Pac J Cancer Prev

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Background: MicroRNAs (miRNAs) are non-coding RNAs that regulate multiple cellular processes during cancer progression, identified to be involved in tumorgenesis of several cancers including cancers of digestive system. However its role in gallbladder inflammatory disease (GID) and gallbladder cancer (GBC) has not been well documented. The present study was aimed to investigate the clinical significance of hsa-miRNA-335-5p (miR-335) in GBC and GID. Subjects and Methods: This prospective case control study, conducted from July 1, 2014 to December 1, 2017 in Era's Lucknow Medical College & Hospital, India, evaluated miR-335 expression by real-time polymerase chain reaction. Hundred tissue samples GID (control; n=50) and GBC (case; n=50) were studied. Relative quantification of target miR-335 expression was examined using the comparative cycle threshold method. Their expression was correlated with different clinicopathological parameters. Fishers' exact test, Student's t-test, and Chi-square test were used as appropriate for data analysis. Kaplan-Meier methods were used to calculate overall and disease-free survival rate. Two sided P<0.05 was considered as significant. Results: miR-335 expression was found to be significantly low in GBC lesions when compared with GID lesions (P<0.001). The low expression level of miR-335 was correlated with histological grade (P=0.007), clinical stage (P<0.001), lymph node metastasis (P<0.001) and liver metastasis (P=0.016). Reduced expression of miRNA-335 was associated with a shorter median overall survival (7 months vs. 25 months) in GBC patients (P<0.001). Conclusions: Down regulation of miR-335 is associated with the severity of the disease and thus indicate that miR-335 expression may serve as prognostic marker for GBC.

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Long non-coding RNA MSTO2P promotes the proliferation and colony formation in gastric cancer by indirectly regulating miR-335 expression

Cited by 18 publications

References 35 publications

miR‐335 inhibited cell proliferation of lung cancer cells by target Tra2β

miR‐335 inhibited cell proliferation of lung cancer cells by target Tra2β

Prognostic model based on autophagy-related lncRNAs in gastric cancer

Low Expression of MicroRNA335-5p Is Associated with Malignant Behavior of Gallbladder Cancer: A Clinicopathological Study

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