Long Non-coding RNA NEAT1 as an Emerging Biomarker in Breast and Gynecologic Cancers: a Systematic Overview

Thankachan, Sanu; Bhardwaj, Boddapati Kalyani; Venkatesh, Thejaswini; Suresh, Padmanaban S.

doi:10.1007/s43032-021-00481-x

Cited by 10 publications

(11 citation statements)

References 104 publications

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“…In recent years, more and more studies have shown that lncRNAs can exert their biological functions by regulating the expression of miRNAs, thus affecting the occurrence and development of uterine diseases ( Ghafouri-Fard et al, 2020 ; Thankachan et al, 2021 ). Therefore, the Starbase v3.0 database was used to predict the potential miRNA binding sites in lncRNA-MIAT.…”

Section: Discussionmentioning

confidence: 99%

“…miRNAs participate in essential functions in various biological regulatory pathways including cell apoptosis, differentiation, and proliferation ( Kanekura et al, 2016 ; Klinge, 2018 ). In recent years, more and more studies have shown that lncRNAs can exert their biological functions by regulating the expression of miRNAs, thus affecting the occurrence and development of uterine diseases ( Ghafouri-Fard et al, 2020 ; Thankachan et al, 2021 ). Several studies have demonstrated that lncRNAs may be involved in the formation of competitive endogenous RNA (ceRNA) regulatory networks, and there may be a negative correlation between lncRNA and miRNA ( Vallone et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

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ADSC Exosomes Mediate lncRNA-MIAT Alleviation of Endometrial Fibrosis by Regulating miR-150-5p

Shao

Qin

Wan³

et al. 2021

Front. Genet.

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BackgroundSecondary infertility remains a major complication of endometrial fibrosis in women. The use of exosomes from adipose-derived mesenchymal stem cells (ADSCs) has shown promising results for the treatment of endometrial fibrosis. However, the mechanisms of action of ADSC-exosome (ADSC-Exo) therapy remain unclear.Materials and MethodsAn endometrial fibrosis model was established in mice treated with alcohol and endometrial epithelial cells (ESCs) treated with TGF-β1. ADSCs were isolated from Sprague Dawley (SD) rats, and exosomes were isolated from ADSCs using ExoQuick reagent. Exosomes were identified by transmission electron microscopy (TEM), NanoSight, and Western blot analysis. The expression level of lncRNA-MIAT was detected by qPCR analysis. Western blot analysis was carried out to determine the protein levels of fibrosis markers (TGFβR1, α-SMA, and CK19). A dual-luciferase reporter gene assay was used to verify the relationship between target genes. The endometrial tissues of the endometrial fibrosis model were stained with HE and Masson’s trichrome.ResultsADSCs and ADSC-Exos were successfully isolated, and the expression level of lncRNA-MIAT was significantly down-regulated in endometrial tissue and the TGF-β1-induced ESC injury model, whereas ADSC-Exos increased the expression of lncRNA-MIAT in the TGF-β1-induced ESC model. Functionally, ADSC-Exo treatment repressed endometrial fibrosis in vivo and in vitro by decreasing the expression of hepatic fibrosis markers (α-SMA and TGFβR1) and increasing the expression of CK19. Moreover, miR-150-5p expression was repressed by lncRNA-MIAT in the TGF-β1-induced ESC injury model. The miR-150-5p mimic promoted TGF-β1-induced ESC fibrosis.ConclusionADSC-Exos mediate lncRNA-MIAT alleviation of endometrial fibrosis by regulating miR-150-5p, which suggests that lncRNA-MIAT from ADSC-Exos may be a viable treatment for endometrial fibrosis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

ADSC Exosomes Mediate lncRNA-MIAT Alleviation of Endometrial Fibrosis by Regulating miR-150-5p

Shao

Qin

Wan³

et al. 2021

Front. Genet.

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“…Many papers have addressed the association of elevated plasma IL-18 level with mortality in ARDS (Makabe et al, 2012;Dong et al, 2019;Rogers et al, 2019), emphasizing the connection between NLRP3 inflammasome and COVID-19 progression. Indeed, accumulating evidence has shown that the Spike (S) protein from SARS-CoV-2 can trigger IL-1β secretion from the NLRP3 inflammasome in macrophages (Theobald et al, 2021) and increase NF-κB nuclear translocation, NLRP3 expression levels and caspase-1 activity in PBMCs (Olajide et al, 2021). Meanwhile, the ssRNA from SARS-CoV-2 triggers a greater inflammatory response than SARS-CoV-1 and HIV-1 (Campbell et al, 2021), as cell death induced by these cytokines also releases more PAMPs/ DAMPs, thus leading to both local and systematic NLRP3 hyperactivation as well as a positive feedback loop of cytokine signaling, which results in eventually lethal damage to the whole body (Channappanavar et al, 2016;Fu et al, 2020).…”

Section: Hyperactivation Of the Nlrp3 Inflammasomementioning

confidence: 99%

Assessing the suitability of long non-coding RNAs as therapeutic targets and biomarkers in SARS-CoV-2 infection

Zhong

Ashley

Steain

et al. 2022

Front. Mol. Biosci.

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Long non-coding RNAs (lncRNAs) are RNA transcripts that are over 200 nucleotides and rarely encode proteins or peptides. They regulate gene expression and protein activities and are heavily involved in many cellular processes such as cytokine secretion in respond to viral infection. In severe COVID-19 cases, hyperactivation of the immune system may cause an abnormally sharp increase in pro-inflammatory cytokines, known as cytokine release syndrome (CRS), which leads to severe tissue damage or even organ failure, raising COVID-19 mortality rate. In this review, we assessed the correlation between lncRNAs expression and cytokine release syndrome by comparing lncRNA profiles between COVID-19 patients and health controls, as well as between severe and non-severe cases. We also discussed the role of lncRNAs in CRS contributors and showed that the lncRNA profiles display consistency with patients’ clinic symptoms, thus suggesting the potential of lncRNAs as drug targets or biomarkers in COVID-19 treatment.

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“…Given the role of NEAT1 in postnatal mammary gland development, it is perhaps not surprising that NEAT1 is abnormally expressed in breast cancer. 108 The first report on NEAT1 and breast cancer was published in 2014 showing that NEAT1 expression and paraspeckle formation were upregulated in hypoxic regions of breast cancer cell line xenografts. 67 Moreover, the authors reported that high NEAT1 expression is associated with poor clinical outcome in a breast cancer cohort of ~2000 patients regardless of clinicopathological conditions like age, tumour size, stage, grade, and lymph node metastases.…”

Section: Neat1 Is Frequently Upregulated In Breast Cancermentioning

confidence: 99%

Expression and functions of long non-coding RNA NEAT1 and isoforms in breast cancer

2021

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NEAT1 is a highly abundant nuclear architectural long non-coding RNA. There are two overlapping NEAT1 isoforms, NEAT1_1 and NEAT1_2, of which the latter is an essential scaffold for the assembly of a class of nuclear ribonucleoprotein bodies called paraspeckles. Paraspeckle formation is elevated by a wide variety of cellular stressors and in certain developmental processes, either through transcriptional upregulation of the NEAT1 gene or through a switch from NEAT1_1 to NEAT1_2 isoform production. In such conditions, paraspeckles modulate cellular processes by sequestering proteins or RNA molecules. NEAT1 is abnormally expressed in many cancers and a growing body of evidence suggests that in many cases, high NEAT1 levels are associated with therapy resistance and poor clinical outcome. Here, we review the current knowledge of NEAT1 expression and functions in breast cancer, highlighting its established role in postnatal mammary gland development. We will discuss possible isoform-specific roles of NEAT1_1 and NEAT1_2 in different breast cancer subtypes, which critically needs to be considered when studying NEAT1 and breast cancer.

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Long Non-coding RNA NEAT1 as an Emerging Biomarker in Breast and Gynecologic Cancers: a Systematic Overview

Cited by 10 publications

References 104 publications

ADSC Exosomes Mediate lncRNA-MIAT Alleviation of Endometrial Fibrosis by Regulating miR-150-5p

ADSC Exosomes Mediate lncRNA-MIAT Alleviation of Endometrial Fibrosis by Regulating miR-150-5p

Assessing the suitability of long non-coding RNAs as therapeutic targets and biomarkers in SARS-CoV-2 infection

Expression and functions of long non-coding RNA NEAT1 and isoforms in breast cancer

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