Long non-coding RNA PRNCR1 promotes ovarian cancer cell proliferation, migration and invasion by targeting the miR-653-5p/ELF2 axis

Xing, Qi; Chen, Dejun; Yu, Wei-chang; Wang, Liming; Liu, Lu; Tao, Xiao-Ling

doi:10.1007/s11010-022-04371-x

Cited by 9 publications

(4 citation statements)

References 26 publications

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“…S6B–F ). On the contrary, miR-653-5p exhibited a universally suppressive effect on the progression of various cancers [ 33 , 34 ]. Moreover, we detected that there existed a negative correlation between the expression of circTBPL1 and miR-653-5p in breast cancer cells (Supplementary Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Although no statistical significance was found in the correlation analysis, the expression of miR-653-5p was negatively related with circTBPL1 in breast cancer cells, which might attribute to the limited number of cells. Interestingly, miR-653-5p was identified as a general sponging target for various non-coding RNAs, exerting a tumor-suppressing effect in many cancer types, such as prostate cancer [ 33 ], ovarian cancer [ 34 ], and melanoma [ 52 ]. Consist with the previous reports, miR-653-5p overexpression could significantly inhibit cell viability and motility, confirming the tumor-repressive role of miR-653-5p in breast cancer.…”

Section: Discussionmentioning

confidence: 99%

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Cancer-associated fibroblasts facilitate breast cancer progression through exosomal circTBPL1-mediated intercellular communication

Liang

Wang

et al. 2023

Cell Death Dis

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Breast cancer is the major common malignancy worldwide among women. Previous studies reported that cancer-associated fibroblasts (CAFs) showed pivotal roles in regulating tumor progression via exosome-mediated cellular communication. However, the detailed mechanism underlying the exosomal circRNA from CAFs in breast cancer progression remains ambiguous. Here, exosomal circRNA profiling of breast cancer-derived CAFs and normal fibroblasts (NFs) was detected by high-throughput sequencing, and upregulated circTBPL1 expression was identified in CAF exosomes. The exosomal circTBPL1 from CAFs could be transferred to breast cancer cells and promoted cell proliferation, migration, and invasion. Consistently, circTBPL1 knockdown in CAFs attenuated their tumor-promoting ability. Further exploration identified miR-653-5p as an inhibitory target of circTBPL1, and ectopic expression of miR-653-5p could partially reverse the malignant phenotypes induced by circTBPL1 overexpression in breast cancer. Additionally, TPBG was selected as a downstream target gene, and circTBPL1 could protect TPBG from miR-653-5p-mediated degradation, leading to enhanced breast cancer progression. Significantly, the accelerated tumor progression triggered by exosomal circTBPL1 from CAFs was confirmed in xenograft models. Taken together, these results revealed that exosomal circTBPL1 derived from CAFs contributed to cancer progression via miR-653-5p/TPBG pathway, indicating the potential of exosomal circTBPL1 as a biomarker and novel therapeutic target for breast cancer.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Cancer-associated fibroblasts facilitate breast cancer progression through exosomal circTBPL1-mediated intercellular communication

Liang

Wang

et al. 2023

Cell Death Dis

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“…For instance, a recent study shows that exosomal miR-653-5p derived from mesenchymal stem cells suppresses laryngeal papilloma progression [ 21 ]. In addition, miR-653-5p plays a role in cell proliferation in various cancer cells, such as ovarian cancer [ 22 ], papillary thyroid carcinoma [ 23 ], breast cancer [ 24 , 25 , 26 ], gastric cancer [ 27 ], and lung cancer [ 28 , 29 ]. Although miR-374c-5p is less well characterized, recent studies show that miR-374c-5p secreted from mesenchymal stem cells inhibits cancer growth and metastasis formation by regulating the epithelial–mesenchymal transition [ 30 , 31 ].…”

Section: Discussionmentioning

confidence: 99%

Genes Related to Frontonasal Malformations Are Regulated by miR-338-5p, miR-653-5p, and miR-374-5p in O9-1 Cells

Iwaya,

Yu,

Iwata

2024

JDB

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Frontonasal malformations are caused by a failure in the growth of the frontonasal prominence during development. Although genetic studies have identified genes that are crucial for frontonasal development, it remains largely unknown how these genes are regulated during this process. Here, we show that microRNAs, which are short non-coding RNAs capable of targeting their target mRNAs for degradation or silencing their expression, play a crucial role in the regulation of genes related to frontonasal development in mice. Using the Mouse Genome Informatics (MGI) database, we curated a total of 25 mouse genes related to frontonasal malformations, including frontonasal hypoplasia, frontonasal dysplasia, and hypotelorism. MicroRNAs regulating the expression of these genes were predicted through bioinformatic analysis. We then experimentally evaluated the top three candidate miRNAs (miR-338-5p, miR-653-5p, and miR-374c-5p) for their effect on cell proliferation and target gene regulation in O9-1 cells, a neural crest cell line. Overexpression of these miRNAs significantly inhibited cell proliferation, and the genes related to frontonasal malformations (Alx1, Lrp2, and Sirt1 for miR-338-5p; Alx1, Cdc42, Sirt1, and Zic2 for miR-374c-5p; and Fgfr2, Pgap1, Rdh10, Sirt1, and Zic2 for miR-653-5p) were directly regulated by these miRNAs in a dose-dependent manner. Taken together, our results highlight miR-338-5p, miR-653-5p, and miR-374c-5p as pathogenic miRNAs related to the development of frontonasal malformations.

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“…Hsa‐miR‐216a, hsa‐miR‐3684, hsa‐miR‐4437, hsa‐miR‐641, and hsa‐miR‐552 have been reported to play suppressive or promotive roles in cancer. Hsa‐miR‐653 has been revealed to inhibit cancer progression in hepatocellular carcinoma, prostate cancer, breast cancer, papillary thyroid carcinoma, ovarian cancer, and gastric cancer, 59 , 60 , 61 , 62 , 63 , 64 while Liu et al reported that miR‐653 enhances CRC progression by targeting circSETD3/KLF6 axis recently. 65 Why does miR‐653 play an opposite role in CRC?…”

Section: Discussionmentioning

confidence: 99%

Cuproptosis‐related miRNAs signature and immune infiltration characteristics in colorectal cancer

et al. 2023

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BackgroundA novel form of cell death termed cuproptosis was proposed recently. miRNAs play important roles in colorectal cancer (CRC). However, their relationships have not been reported.MethodsmiRNAs that negatively regulate 16 cuproptosis regulators were predicted using Targetscan database. The univariate Cox, LASSO, and multivariate Cox regression analyses were performed to select cuproptosis‐related miRNAs. GSEA and ssGSEA analysis was carried out for functional enrichment analysis. The immune cell proportion score (IPS) and the efficiencies of multiple chemotherapy drugs were compared between different risk groups. The CCK8, cell colony, edu, and flow cytometry assays were performed to validate the roles of miRNA. Luciferase reporter assay confirmed the regulatory mechanism of miRNA on cuproptosis.ResultsSix cuproptosis‐related miRNAs (hsa‐miR‐653, hsa‐miR‐216a, hsa‐miR‐3684, hsa‐miR‐4437, hsa‐miR‐641, and hsa‐miR‐552) were screened out for model construction. The risk score could act as an independent prognostic indicator in CRC (p < 0.001, 95% HR = 1.243 (1.129–1.369)). The nomogram could efficiently predict the overall survival rate (AUC = 0.836). Then, the level of immunosuppressive pathways, immunosuppressive cells, stromal‐activated genes, and stromal score was higher in the high‐risk group. The IPS analysis showed a better response to immunotherapy in the low‐risk group. Also, the risk score was closely correlated with efficiencies of multiple chemotherapy drugs. Furthermore, miR‐653 was highly expressed in CRC tissues (p < 0.001), closely correlated with T stage (p < 0.001), metastasis (p < 0.001), and tumor stage (p < 0.001). High expression of miR‐653 predicted a shorter overall survival (p = 0.0282) and disease‐free survival (p = 0.0056). In addition, miR‐653 promoted cell proliferation, inhibited apoptosis, and negatively regulated the expression of DLD through directly binding to the 3’‐UTR of DLD mRNA.ConclusionWe constructed a cuproptosis‐related miRNA signature for the prediction of CRC patient survival and immunotherapy sensitivity. miR‐653 was highly expressed in CRC tissues, promoted cell proliferation, and inhibited apoptosis by negatively regulating the expression of DLD.

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Long non-coding RNA PRNCR1 promotes ovarian cancer cell proliferation, migration and invasion by targeting the miR-653-5p/ELF2 axis

Cited by 9 publications

References 26 publications

Cancer-associated fibroblasts facilitate breast cancer progression through exosomal circTBPL1-mediated intercellular communication

Cancer-associated fibroblasts facilitate breast cancer progression through exosomal circTBPL1-mediated intercellular communication

Genes Related to Frontonasal Malformations Are Regulated by miR-338-5p, miR-653-5p, and miR-374-5p in O9-1 Cells

Cuproptosis‐related miRNAs signature and immune infiltration characteristics in colorectal cancer

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