Long Non-Coding RNA TRIM52-AS1 Promotes Growth and Metastasis via miR-218-5p/ROBO1 in Hepatocellular Carcinoma

Liu, Yuanjun; Wu, Yuzhang; Liu, Shuang; Dai, Yi

doi:10.2147/cmar.s286205

Cited by 10 publications

(12 citation statements)

References 40 publications

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“…Studies on hepatocellular carcinoma (HCC) have shown that the TRIM52-AS1 knockdown inhibits the proliferation and metastasis of HCC cells. TRIM52-AS1 behaves as a competitive endogenous RNA (ceRNA) and promotes the progression of HCC by sponging the miRNAs and up-regulating the expression of the mRNAs that are regulated by miRNAs [ 49 , 50 ]. Therefore, it can be concluded that TRIM52-AS is down-regulated in CC tissues; this attenuates the sponging effect on miR-378a, and increases the binding of miR-378a to GZMB, which in turn inhibits and down-regulates the expression of GZMB.…”

Section: Discussionmentioning

confidence: 99%

Identification of pyroptosis-related signature for cervical cancer predicting prognosis

Zhou

Zheng

et al. 2021

Aging

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Cervical cancer (CC) is one of the most common malignancies encountered in gynecology practice. However, there is a paucity of information about specific biomarkers that assist in the diagnosis and prognosis of CC. Pyroptosis is a form of programmed cell death whose different elements are related to the occurrence, invasion, and metastasis of tumors. However, the role of pyroptosis phenomena in the progression of CC has not yet been elucidated. This study focuses on the development of a pyroptosis-associated prognostic signature for CC using integrated bioinformatics to delineate the relationships among the signature, tumor microenvironment, and immune response of the patients. In this respect, we identified a prognostic signature that depends on eight pyroptosis-related genes (PRGs) that designate with better prognostic survival in the low-risk group (P<0.05) and where AUC values were greater than 0.7. A multi-factor Cox regression analysis indicated that such a signature could be used as an independent prognostic factor, and both the DCA and the Nomogram suggested that the proposed prognostic signature had good predictive capabilities. Interestingly, this prognostic signature can be applied to multiple tumors and thus, is versatile from a clinical point of view. In addition, there were significant differences in the tumor microenvironment and immune infiltration status between the high- and low-risk groups (P<0. 05). The core gene granzyme B (GZMB) was screened and the CC-associated regulatory axis, GZMB/ miR-378a/TRIM52-AS1, was constructed, which may promote CC progression, and further experimentation is needed to validate these results.

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Section: Discussionmentioning

confidence: 99%

Identification of pyroptosis-related signature for cervical cancer predicting prognosis

Zhou

Zheng

et al. 2021

Aging

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“…The TRIM52 protein belongs to the C-V isoform of the TRIM protein family, with 1 RING structure at a time from the N-terminal to the C-terminal, a B-Box2 structural domain, and lacking a distinct double helix structural domain and C-terminus. It has been shown as a promoter in ovarian cancer (12), hepatocellular carcinoma (13,15), and renal cancer (14), while its role in the development of CC had not been clarified Our study demonstrated that TRIM52 is significantly highly expressed in CC cell lines. Cell proliferation and cell cycle showed a statistically significant decrease of approximately 20.83% in the proportion of S-phase cells in HCT116 cells following knockdown of TRIM52.…”

Section: Discussionmentioning

confidence: 63%

“…Non-coding (lnc)RNA TRIM52-AS1 knockdown inhibited the proliferation and metastasis of hepatocellular carcinoma cells. It has been shown to promote the growth and metastasis of hepatocellular carcinoma via miR-218-5p/ROBO1 (15). It was reported that TRIM52 upregulation promotes proliferation and invasiveness through activation of the Wnt/β-catenin pathway in lung cancer cells (16).…”

Section: Introductionmentioning

confidence: 99%

Tripartite motif 52 (TRIM52) promotes proliferation, migration, and regulation of colon cancer cells associated with the NF-κB signaling pathway

Guo¹,

Zhou²,

Gu³

et al. 2022

J Gastrointest Oncol

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Background: With the advancement of early detection and treatment, the incidence of colon cancer (CC) has declined steadily worldwide; however, the mortality remains unacceptably high. Tripartite motif 52 (TRIM52) is a member of the family of highly conserved RBCC (a RING-finger, two B-boxes, and a predicted alpha-helical Coiled-Coil domain were linked to the N-terminal region in sequence) proteins with more than 70 isoforms, which plays an important role in tumorigenesis through different signaling pathways.How it regulates the development of CC remains unknown.Methods: Western blot was used to reveal that TRIM52 protein expression is up-regulated in CC cells.The Analysis of The Cancer Genome Atlas (TCGA) database was used to find the different expressions of TRIM52 between colon cancer tissues and normal colonic epithelial tissues. Cell proliferation assays, migration and invasion assays, and apoptosis were used to verify the changes in cell function after knockdown or overexpression of TRIM52 in CC cells. After that, the key proteins of the nuclear factor (NF)-κB signaling pathway were validated by western blot to explore the role of TRIM52 in the NF-κB signaling pathway.Finally, in order to explore the potential sites of TRIM52, LPS and PDTC were employed to activate and block the NF-κB signaling pathway, and the key proteins of the NF-κB signaling pathway were validated by western blot.Results: TGCA database revealed that TRIM52 expression was elevated in CC tissues and correlated with prognosis. It was verified that TRIM52 promoted the proliferation, migration, and invasion of CC cells, and inhibited cell apoptosis. Most of the tripartite motif proteins (TRIMs) have ubiquitin ligase activity related to their highly conserved RING structure. Detection of the key proteins of the NF-κB signaling pathway in CC cells revealed that TRIM52 activated the NF-κB signaling pathway. Conclusions:We confirmed that TRIM52 promotes proliferation, migration, and invasion while inhibiting apoptosis of CC cells. The regulatory effect of TRIM52 on CC cells is related to the activation of the NF-κB signaling pathway. As TRIM52 acted as an upstream stimulator, stimulating the transfer of P65 into the nucleus to activate the NF-κB signaling pathway, it may provide a potential target for prognosis prediction and treatment of CC.

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“…While little is known about the role of SLIT3 in the placenta, SLIT2 and its receptor, roundabout guidance receptor 1 (ROBO1), have been suggested to regulate trophoblast proliferation and differentiation and their aberrant signaling may be associated with various pregnancy-related disorders ( Li et al, 2015 ; Li et al, 2017 ; Tiensuu et al, 2019 ). Interestingly, ROBO1, which is a functional target of miR-218-5p ( Liu Y. et al, 2021 ), is upregulated in preeclamptic placentas ( Liao et al, 2012 ).…”

Section: Microrna Regulation Of Trophoblast Invasion Spiral Artery Re...mentioning

confidence: 99%

Role of microRNAs in trophoblast invasion and spiral artery remodeling: Implications for preeclampsia

Hayder

Shan²,

Chen³

et al. 2022

Front. Cell Dev. Biol.

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It is now well-established that microRNAs (miRNAs) are important regulators of gene expression. The role of miRNAs in placental development and trophoblast function is constantly expanding. Trophoblast invasion and their ability to remodel uterine spiral arteries are essential for proper placental development and successful pregnancy outcome. Many miRNAs are reported to be dysregulated in pregnancy complications, especially preeclampsia and they exert various regulatory effects on trophoblasts. In this review, we provide a brief overview of miRNA biogenesis and their mechanism of action, as well as of trophoblasts differentiation, invasion and spiral artery remodeling. We then discuss the role of miRNAs in trophoblasts invasion and spiral artery remodeling, focusing on miRNAs that have been thoroughly investigated, especially using multiple model systems. We also discuss the potential role of miRNAs in the pathogenesis of preeclampsia.

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Long Non-Coding RNA TRIM52-AS1 Promotes Growth and Metastasis via miR-218-5p/ROBO1 in Hepatocellular Carcinoma

Cited by 10 publications

References 40 publications

Identification of pyroptosis-related signature for cervical cancer predicting prognosis

Identification of pyroptosis-related signature for cervical cancer predicting prognosis

Tripartite motif 52 (TRIM52) promotes proliferation, migration, and regulation of colon cancer cells associated with the NF-κB signaling pathway

Role of microRNAs in trophoblast invasion and spiral artery remodeling: Implications for preeclampsia

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