Long non-coding RNAs in regulation of adipogenesis and adipose tissue function

Squillaro, Tiziana; Peluso, Gianfranco; Galderisi, Umberto; Bernardo, Giovanni Di

doi:10.7554/elife.59053

Cited by 64 publications

(49 citation statements)

References 96 publications

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“…MSCs are present within WAT depots. These cells contain several sub-populations, which comprise multipotent stem cells (able to produce adipocytes osteocytes and chondrocytes), fibroblasts, stromal cells, and pro-genitor cells [ 22 , 23 ]. Our in vitro findings identified two compounds that may warrant further in vivo investigation as possible anti-obesity drugs.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Browning Agents on the White Adipogenesis of Bone Marrow Mesenchymal Stromal Cells: A Contribution to Fighting Obesity

Maio

Alessio

Demirsoy

et al. 2021

Cells

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Brown-like adipocytes can be induced in white fat depots by a different environmental or drug stimuli, known as “browning” or “beiging”. These brite adipocytes express thermogenin UCP1 protein and show different metabolic advantages, such as the ability to acquire a thermogenic phenotype corresponding to standard brown adipocytes that counteracts obesity. In this research, we evaluated the effects of several browning agents during white adipocyte differentiation of bone marrow-derived mesenchymal stromal cells (MSCs). Our in vitro findings identified two compounds that may warrant further in vivo investigation as possible anti-obesity drugs. We found that rosiglitazone and sildenafil are the most promising drug candidates for a browning treatment of obesity. These drugs are already available on the market for treating diabetes and erectile dysfunction, respectively. Thus, their off-label use may be contemplated, but it must be emphasized that some severe side effects are associated with use of these drugs.

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Section: Introductionmentioning

confidence: 99%

Evaluation of Browning Agents on the White Adipogenesis of Bone Marrow Mesenchymal Stromal Cells: A Contribution to Fighting Obesity

Maio

Alessio

Demirsoy

et al. 2021

Cells

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“…Recent studies have addressed the role of lncRNAs in the network governing adipogenesis and adipocytes biology. Some lncRNAs play a key role in these phenomena by interacting with different transcription factors, such as PGC-1alpha, EBF2, ZBTB7B, ZFP516, and PRDM16 [ 31 ]. Moreover, lncRNAs could play a remarkable role in explaining gender differences.…”

Section: Introductionmentioning

confidence: 99%

RNA-seq Characterization of Sex-Differences in Adipose Tissue of Obesity Affected Patients: Computational Analysis of Differentially Expressed Coding and Non-Coding RNAs

Rey

Messa

Pandini³

et al. 2021

JPM

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Obesity is a multifactorial disease presenting sex-related differences including adipocyte functions, sex hormone effects, genetics, and metabolic inflammation. These can influence individuals’ risk for metabolic dysfunctions, with an urgent need to perform sex-based analysis to improve prevention, treatment, and rehabilitation programs. This research work is aimed at characterizing the transcriptional differences present in subcutaneous adipose tissue (SAT) of five obesity affected men versus five obesity affected women, with an additional focus on the role of long non-coding RNAs. Through RNA-sequencing, we highlighted the presence of both coding and non-coding differentially expressed RNAs, and with numerous computational analyses we identified the processes in which these genes are implicated, along with their role in co-morbidities development. We report 51 differentially expressed transcripts, 32 of which were coding genes and 19 were non-coding. Using the WGCNA R package (Weighted Correlation Network Analysis, version 1.70-3), we describe the interactions between coding and non-coding RNAs, and the non-coding RNAs association with the insurgence of specific diseases, such as cancer development, neurodegenerative diseases, and schizophrenia. In conclusion, our work highlights a specific gender sex-related transcriptional signature in the SAT of obesity affected patients.

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“…The molecular mechanisms underlying the influence of obesity on the development and progression of cancer are not yet completely defined, and their characterization could highlight new mechanisms leading to increased susceptibility to cancer. Transcriptional characterization of specific tissues in obese patients is of crucial relevance in highlighting new key players and a relevant focus should be placed on lncRNAs, as emerging evidence links them to numerous obesity-related disorders and multiple types of cancer [ 80 , 81 , 82 , 83 , 84 , 85 , 86 , 87 , 88 , 89 ]. In order to evaluate the role of adipose tissue gene expression in tumor development, we aimed to evaluate the presence of coding and non-coding oncogenes and cancer-associated pathways in obesity-affected subjects.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis of Subcutaneous Adipose Tissue from Severely Obese Patients Highlights Deregulation Profiles in Coding and Non-Coding Oncogenes

Rey

Messa

Pandini

et al. 2021

IJMS

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Obesity is a major risk factor for a large number of secondary diseases, including cancer. Specific insights into the role of gender differences and secondary comorbidities, such as type 2 diabetes (T2D) and cancer risk, are yet to be fully identified. The aim of this study is thus to find a correlation between the transcriptional deregulation present in the subcutaneous adipose tissue of obese patients and the oncogenic signature present in multiple cancers, in the presence of T2D, and considering gender differences. The subcutaneous adipose tissue (SAT) of five healthy, normal-weight women, five obese women, five obese women with T2D and five obese men were subjected to RNA-sequencing, leading to the identification of deregulated coding and non-coding RNAs, classified for their oncogenic score. A panel of DE RNAs was validated via Real-Time PCR and oncogene expression levels correlated the oncogenes with anthropometrical parameters, highlighting significant trends. For each analyzed condition, we identified the deregulated pathways associated with cancer, the prediction of possible prognosis for different cancer types and the lncRNAs involved in oncogenic networks and tissues. Our results provided a comprehensive characterization of oncogenesis correlation in SAT, providing specific insights into the possible molecular targets implicated in this process. Indeed, the identification of deregulated oncogenes also in SAT highlights hypothetical targets implicated in the increased oncogenic risk in highly obese subjects. These results could shed light on new molecular targets to be specifically modulated in obesity and highlight which cancers should receive the most attention in terms of better prevention in obesity-affected patients.

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Long non-coding RNAs in regulation of adipogenesis and adipose tissue function

Cited by 64 publications

References 96 publications

Evaluation of Browning Agents on the White Adipogenesis of Bone Marrow Mesenchymal Stromal Cells: A Contribution to Fighting Obesity

Evaluation of Browning Agents on the White Adipogenesis of Bone Marrow Mesenchymal Stromal Cells: A Contribution to Fighting Obesity

RNA-seq Characterization of Sex-Differences in Adipose Tissue of Obesity Affected Patients: Computational Analysis of Differentially Expressed Coding and Non-Coding RNAs

Transcriptome Analysis of Subcutaneous Adipose Tissue from Severely Obese Patients Highlights Deregulation Profiles in Coding and Non-Coding Oncogenes

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