Long noncoding RNA AFAP1‑AS1 is upregulated in NSCLC and associated with lymph node metastasis and poor prognosis

Leng, Xuechun; Ding, Xiangxiang; Wang, Siwei; Tian, Fang; Shen, Wenrong; Xia, Wenjia; You, Ran; Xu, Kan; Yin, Rong

doi:10.3892/ol.2018.8784

Cited by 14 publications

(13 citation statements)

References 29 publications

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“…AFAP1-AS1 expression is positively correlated with tumor pathological grade, TNM staging, smoking history, infiltration degree, distant metastasis, and clinical outcomes in NSCLC patients [91,99,100,101]. High expression of AFAP1-AS1 is correlated with advanced lymph node metastasis and reduced survival time [102]. A meta-analysis report showed that increased expression level of AFAP1-AS1 is a strong predictor of poor OS in NSCLC patients [91].…”

Section: Lncrnas In Lung Cancer (Lc)mentioning

confidence: 99%

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Long Non-Coding RNA in the Pathogenesis of Cancers

Chi

Wang

et al. 2019

Cells

646

452

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The incidence and mortality rate of cancer has been quickly increasing in the past decades. At present, cancer has become the leading cause of death worldwide. Most of the cancers cannot be effectively diagnosed at the early stage. Although there are multiple therapeutic treatments, including surgery, radiotherapy, chemotherapy, and targeted drugs, their effectiveness is still limited. The overall survival rate of malignant cancers is still low. It is necessary to further study the mechanisms for malignant cancers, and explore new biomarkers and targets that are more sensitive and effective for early diagnosis, treatment, and prognosis of cancers than traditional biomarkers and methods. Long non-coding RNAs (lncRNAs) are a class of RNA transcripts with a length greater than 200 nucleotides. Generally, lncRNAs are not capable of encoding proteins or peptides. LncRNAs exert diverse biological functions by regulating gene expressions and functions at transcriptional, translational, and post-translational levels. In the past decade, it has been demonstrated that the dysregulated lncRNA profile is widely involved in the pathogenesis of many diseases, including cancer, metabolic disorders, and cardiovascular diseases. In particular, lncRNAs have been revealed to play an important role in tumor growth and metastasis. Many lncRNAs have been shown to be potential biomarkers and targets for the diagnosis and treatment of cancers. This review aims to briefly discuss the latest findings regarding the roles and mechanisms of some important lncRNAs in the pathogenesis of certain malignant cancers, including lung, breast, liver, and colorectal cancers, as well as hematological malignancies and neuroblastoma.

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Section: Lncrnas In Lung Cancer (Lc)mentioning

confidence: 99%

“…AFAP1-AS1 is upregulated in NSCLC tissues and cell lines [91,99,100,101,102,103,104,105,106,107]. Inhibition of AFAP-AS1 suppresses cell growth and migration and promotes apoptosis of NSCLC cells in vitro [103,104].…”

Section: Lncrnas In Lung Cancer (Lc)mentioning

confidence: 99%

Long Non-Coding RNA in the Pathogenesis of Cancers

Chi

Wang

et al. 2019

Cells

646

452

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“…Long non‐coding RNAs (lncRNAs) have been demonstrated to play essential role in the development of malignant tumors . They were suggested to involve in various biological modifications including epigenetic modification and transcriptional/post‐transcriptional regulation .…”

Section: Introductionmentioning

confidence: 99%

Long non‐coding RNA HCG11 suppresses the growth of glioma by cooperating with the miR‐4425/MTA3 axis

Zhang

Cao

Kou

et al. 2019

The Journal of Gene Medicine

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Background Glioma is a type of malignant tumor that occurs in the central nervous system of adults. Long non‐coding RNAs (lncRNAs) that potentially participate in the initiation and progression of glioma have been widely reported. As a now‐found lncRNA, HLA complex group 11 (HCG11) has not yet been studied in glioma. The present study aimed to determine the role of HCG11 in the tumorigenesis of glioma. Methods A quantitative real‐time polymerase chain reaction assay was performed to examine the expression pattern of HCG11 in 84 glioma tissues and cell lines. The overall survival rate of glioma patients with a high or low level of HCG11 or metastasis‐associated 1 family member 3 (MTA3) was analyzed by Kaplan–Meier analysis. The effect of HCG11 on glioma cell growth was determined by in vitro and in vivo experiments. MicroRNAs (miRNAs) that potentially interact with HCG11 were searched and determined by bioinformatics analysis and a luciferase reporter assay. Similarly, the target of miRNA‐4425 was identified. Finally, rescue assays were conducted to determine the bio‐function of the competing endogenous RNA pathway. Results HCG11 was downregulated in 84 pairs of glioma tissues and cell lines. Moreover, a low level of HCG11 indicted the lower overall survival rate of glioma patients. Regarding the mechanism, HCG11 was abundant in the cytoplasm of glioma cells and interacted with miR‐4425 to release the expression of MTA3. miR‐4425 and MTA3 participated in HCG11‐mediated glioma growth. Conclusions LncRNA HCG11 suppresses the growth of glioma by cooperating with the miR‐4425/MTA3 axis.

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“…Numerous previous studies have illuminated that noncoding RNAs (ncRNAs), such as long non-coding RNAs (LncRNAs), microRNAs (miRNAs), small nucleolar RNAs (snoRNAs), are emerging as a critical regulator or key elements of cell proliferation and metastasis in human non-small-cell lung cancer (Ji et al 2003;Liao et al 2010;Tang et al 2017). Over the past decades, multiple LncRNAs (defined as > 200 nucleotides in length) have been annotated and identified key roles in cell cycle and regulating gene expression in cancers (Gutschner and Diederichs 2012;Spizzo et al 2012;Shi et al 2013), such as LncRNA HOTAIR (Gupta et al 2010), LncRNA MALAT-1 (Ji et al 2003), LncRNA AFAP1-AS1 (Leng et al 2018), LncRNA NEAT1 (Sun et al 2016), LncRNA TUG1 (Zhang et al 2014). Furthermore, a comprehensive assessment suggested that the dysregulation of lncRNAs has increasingly become an endogenous force in the formation of human cancers (Shi et al 2013;Leng et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Knockdown of LncRNAZFAS1 suppresses cell proliferation and metastasis in non-small cell lung cancer

Zhou

Yang

et al. 2020

Animal Cells and Systems

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To evaluate the effects of LncRNAZFAS1 on cell proliferation and tumor metastasis in non-small cell lung cancer (NSCLC), we detected the expression level of LncRNAZFAS1 in NSCLC-related tissues and cells. qRT-PCR results revealed that LncRNAZFAS1 in tumor tissues was significantly higher than that in normal lung tissue, especially significantly up-regulated in stage III / IV and in metastatic NSCLC tissues. LncRNAZFAS1 expression was dramatically up-regulated in 4 NSCLCrelated cells (A549, SPC-A1, SK-MES-1, and NCI-H1299), with having the highest expression level in A549 cells. Furthermore, we implemented a knockdown of LncRNAZFAS1 in A549 cells, and the results of CCK8 and Transwell assays suggested that knockdown of LncRNAZFAS1 significantly inhibited NSCLC cell proliferation and metastasis. Next, we constructed a tumor xenograft model to evaluate the effect of LncRNAZFAS1 on the NSCLC cell proliferation in vivo. The results indicated that knockdown of LncRNAZFAS1 dramatically inhibited A549 cells proliferation and repressed tumor growth. Additionally, knockdown of LncRNAZFAS1 drastically weakened the expressions of MMP2, MMP9 and Bcl-2 proteins, whereas noticeably strengthened the expression of BAX protein. Our results altogether suggest that knockdown of LncRNAZFAS1 has a negative effect on the proliferation and metastasis of NSCLC cell, which implying LncRNAZFAS1 is a potential unfavorable biomarker in patients with NSCLC.

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Long noncoding RNA AFAP1‑AS1 is upregulated in NSCLC and associated with lymph node metastasis and poor prognosis

Cited by 14 publications

References 29 publications

Long Non-Coding RNA in the Pathogenesis of Cancers

Long Non-Coding RNA in the Pathogenesis of Cancers

Long non‐coding RNA HCG11 suppresses the growth of glioma by cooperating with the miR‐4425/MTA3 axis

Knockdown of LncRNAZFAS1 suppresses cell proliferation and metastasis in non-small cell lung cancer

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