Long noncoding RNA colon cancer associated transcript-1 promotes the proliferation, migration and invasion of cervical cancer

Jia, Lijing; Zhang, Yuan; Tian, Fei; Chu, Zhaoping; Xin, Hong

doi:10.3892/mmr.2017.7302

Cited by 16 publications

(13 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Convincing evidence elucidates the crucial functions and underlying molecular mechanisms of lncRNAs in the physiological and pathological processes of numerous malignancies, including cervical cancer. For instance, depletion of lncRNA colon cancer associated transcript 1 (CCAT1) has been shown to suppress cervical cancer cell viability and migratory and invasive abilities (Jia, Zhang, Tian, Chu, & Xin, ). Silencing of lncRNA steroid receptor RNA activator (SRA) in cervical cancer cell lines impaired cell proliferation, migration and invasion by downregulating epithelial‐to‐mesenchymal transition‐related genes (Eoh et al., ).…”

Section: Discussionmentioning

confidence: 99%

Long non‐coding RNA activated by transforming growth factor‐β promotes proliferation and invasion of cervical cancer cells by regulating the miR‐144/ITGA6 axis

Zhu

Yang

et al. 2019

Experimental Physiology

View full text Add to dashboard Cite

New Findings What is the central question of this study?This study was designed to investigate the molecular mechanism and biological roles of long non‐coding RNA activated by transforming growth factor‐β (lncRNA ATB) in the progression of cervical cancer. What is the main finding and its importance?Our study provided new insight into the cross‐talk between lncRNA ATB, miR‐144 and ITGA6, shedding light on the therapy for cervical cancer. Abstract The present study was designed to investigate the molecular mechanism and biological roles of long non‐coding RNA activated by transforming growth factor‐β (lncRNA ATB) in the progression of cervical cancer. The expression levels of lncRNA ATB, miR‐144 and integrin α6 (ITGA6) were detected in human cervical cancer cell lines using quantitative real‐time PCR and western blotting. Cell viability was quantified by MTT assay at 12, 24, 36, 48 and 72 h after transfection, and cell invasion was determined by the Transwell migration assay. The association among lncRNA ATB, miR‐144 and ITGA6 was disclosed by a dual‐luciferase reporter assay. We found that lncRNA ATB was highly expressed in human cervical cancer cell lines. Further investigation indicated that lncRNA ATB functioned as a competitive endogenous RNA (ceRNA) for miR‐144 to promote cervical cancer cell proliferation and invasion. We demonstrated that ITGA6 was a direct target of miR‐144, and lncRNA ATB facilitated the proliferation and invasion of cervical cancer cells via the miR‐144/ITGA5 axis. In conclusion, the lncRNA ATB/miR‐144/ITGA6 axis might be a promising therapeutic target for cervical cancer.

show abstract

Section: Discussionmentioning

confidence: 99%

Long non‐coding RNA activated by transforming growth factor‐β promotes proliferation and invasion of cervical cancer cells by regulating the miR‐144/ITGA6 axis

Zhu

Yang

et al. 2019

Experimental Physiology

View full text Add to dashboard Cite

show abstract

“…Knockdown of CCAT1 expression inhibited DEX‐mediated protection against OGD/R‐induced cell injury, while overexpression of CCAT1 expression enhanced the Dex‐mediated protection against OGD/R‐induced cell injury. Numerous studies have shown that CCAT1 acts as an oncogene, and is highly expressed in many malignancies, such as colorectal, gastric, ovarian, breast, gallbladder, hepatocellular carcinoma, and lung cancer (Cao et al, ; Gao et al, ; Jia et al, ; Li et al, ; Zhang et al, ; Zhang and Hu, ). CCAT1 exerts its carcinogenic effect by promoting cell invasion and metastasis (Zhao et al, ) and this has been widely studied (Zeng et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…The lncRNA colon cancer‐associated transcript‐1 (lncRNA CCAT1) promotes cell invasion and proliferation and thus plays important roles in the malignant transformation of a variety of tumor tissues. The abnormal levels of CCAT1 are thus, potential markers of tumor prognosis, typically predicting adverse clinical outcomes (Cao et al, ; Gao et al, ; Jia et al, ; Li et al, ; Zhang and Hu, ; Zhang et al, ). However, it is not known if a change in CCAT1 expression occurs in hepatocytes during hepatic ischemia reperfusion injury.…”

Section: Introductionmentioning

confidence: 99%

Dexmedetomidine protects hepatic cells against oxygen‐glucose deprivation/reperfusion injury via lncRNA CCAT1

Zhou

Chen

Wan

et al. 2018

Cell Biology International

View full text Add to dashboard Cite

Dexmedetomidine (Dex) protects different cell types during hypoxia or ischemia-reperfusion injury by inhibiting cell apoptosis. However, the underlying mechanism and its impact on hepatic ischemia reperfusion injury are still not known. In this study, we established a model of oxygen-glucose deprivation/reperfusion (OGD/R) injury in hepatocyte HL7702 cells, and studied the impact of Dex on cell proliferation, apoptosis, and cell cycle during OGD/R. In addition, we explored the role of CCAT1 in this process. We found that Dex increased cell proliferation and inhibited cell apoptosis during OGD/R, in a concentration-dependent manner. Dex partially reversed the OGD-inhibited expression of lncRNA CCAT1. Knockdown of CCAT1 by siRNA inhibited Dex-mediated protection against OGD/R-induced injury and promoted cell apoptosis, caspase-3 expression and cell cycle arrest in the G0/G1 phase, and inhibited cell proliferation and cyclin D1 expression. In contrast, overexpression of CCAT1 by pcDNA3.0-CCAT1 enhanced Dex-mediated protection against OGD/R-induced cell injury. Thus, Dex protects hepatocytes against OGD/R injury by upregulating lncRNA CCAT1. This study suggests a novel role of CCAT1 in ischemia reperfusion injury, and lays the framework for future studies.

show abstract

“…Colon cancer-associated transcript1 (CCAT1) serves as a lncRNA involved in regulating the metastatic ability of various cancers via distinct or similar mechanisms, including colon cancer [ 87 ], cervical cancer [ 88 ], hepatocellular carcinoma [ 89 ] and melanoma [ 90 ] etc. Zhuang et al first uncovered the metastasis-related functions of CCAT1 in HNC.…”

Section: Other Tumor Metastasis-suppressing Lncrnasmentioning

confidence: 99%

Long non-coding RNA implicated in the invasion and metastasis of head and neck cancer: possible function and mechanisms

et al. 2018

View full text Add to dashboard Cite

Head and neck cancer (HNC) ranks as the 6th most common malignancy across the world. Metastasis is a hallmark of cancer, primarily contributing to the relapse and poor prognosis of HNC. Recently, long noncoding RNAs (lncRNAs), previously considered as non-functional, are increasingly appreciated by scholars to play crucial roles in mediating HNC metastasis. LncRNAs, which are located in the nucleus and cytoplasm, mainly exert their function via epigenetic modification, transcriptional control and translational regulation. As several lncRNAs are presently demonstrated to participate in HNC metastasis, we make a summary of the functions and mechanisms regarding these lncRNAs. As shown in the literature, most lncRNAs appear to promote the metastasis of HNC. Hence, we primarily discuss the lncRNAs involved in enhancing metastasis. Additionally, more studies are needed to understand those lncRNAs without clear mechanisms. Furthermore, we introduced the upstream regulator for the aberrant expression of lncRNAs in HNC. Finally, we concisely addressed future research prospects of lncRNAs, particularly the interplay between lncRNAs and tumor immunity as well as lncRNA-targeted therapeutic techniques, and we introduced clustered regularly interspaced short palindromic repeats (CRISPR)-Display as a possibly transformative tool to study lncRNAs. Although lncRNA research is still in the initial stage, it holds great promise to be applied as a prognosticator of HNC and a therapeutic target to inhibit HNC metastasis, which could significantly enhance the outcome of HNC patients.

show abstract

Long noncoding RNA colon cancer associated transcript-1 promotes the proliferation, migration and invasion of cervical cancer

Cited by 16 publications

References 21 publications

Long non‐coding RNA activated by transforming growth factor‐β promotes proliferation and invasion of cervical cancer cells by regulating the miR‐144/ITGA6 axis

Long non‐coding RNA activated by transforming growth factor‐β promotes proliferation and invasion of cervical cancer cells by regulating the miR‐144/ITGA6 axis

Dexmedetomidine protects hepatic cells against oxygen‐glucose deprivation/reperfusion injury via lncRNA CCAT1

Long non-coding RNA implicated in the invasion and metastasis of head and neck cancer: possible function and mechanisms

Contact Info

Product

Resources

About