Long noncoding RNA expression profile in HLE B-3 cells during TGF-β2-induced epithelial-mesenchymal transition

Abstract

BackgroundRecent evidence has shown that long noncoding RNAs (lncRNAs) are involved in the process of epithelial-mesenchymal transition (EMT). However, little research has focused on the expression profile of lncRNAs during EMT in human lens epithelial cells (LECs) and their functions have not yet been described.MethodsDysregulated lncRNAs and mRNAs in normal human lens epithelial B-3(HLE B-3) cells and during transforming growth factor β2(TGF-β2)-induced EMT were analyzed via lncRNA microarray. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathway analyses of differentially expressed mRNAs were performed to identify their functions and pathologic pathways. Six candidate lncRNAs were validated via quantitative real-time reverse transcription polymerase chain reaction(qRT-PCR) to confirm the microarray data.ResultsA total of 775 lncRNAs (325 up-regulated and 450 down-regulated) and 935 mRNAs (329 up-regulated and 606 down-regulated) were differentially expressed in HLE B-3 cells during TGF-β2-induced EMT compared to normal HLE B-3 cells. GO and KEGG Pathway analyses indicated the functions of differentially expressed mRNAs in the TGF-β2-induced EMT in HLE B-3 cells. qRT-PCR confirmed the trends indicated in microarray analysis for all 6 candidate lncRNAs.ConclusionOur study lays the foundation for future research in lncRNAs related to EMT in HLE B-3 cells and could provide new avenues for the prevention and treatment of posterior capsule opacification (PCO).

Long noncoding RNA KCNQ1OT1 promotes proliferation and epithelial‑mesenchymal transition by regulation of SMAD4 expression in lens epithelial cells

“…The procedure was carried out as previously described ( 12 ). Briefly, the collected cataract tissues were treated orderly: 4% paraformaldehyde fixation, paraffin-embedding, section (4 µm thickness), deparaffinization, a descending series of 100, 95, 80 and 70% alcohol were applied for rehydration followed by 3% hydrogen peroxide incubation for 10 min, antigen retrieval, blocking with 10% goat serum (BioWorld Technology, Inc., St. Louis Park, MN, USA), primary antibody incubation (rabbit anti-SMAD4 antibody; 1:100; cat.…”

Long Noncoding RNA MALAT1 Acts as a Competing Endogenous RNA to Regulate TGF-β2 Induced Epithelial-Mesenchymal Transition of Lens Epithelial Cells by a MicroRNA-26a-Dependent Mechanism

“…Recent studies have suggested that the lncRNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1), located on chromosome 11q13, which is involved in the process of EMT in the cancer [8, 9]. Moreover, the emerging evidence has revealed a potential contribution of particular lncRNAs to the development of PCO [10, 11]. However, to the best of our knowledge, whether MALAT1 can regulate EMT of LECs and contribute to the pathogenesis of PCO has not been fully elucidated.…”

The Emerging Roles of Non-Coding RNAs in Cataract

“…Emerging studies reveal that the dysregulation of lncRNAs are impact the development and progression of cataract [31] [32] [33] [34] [35]. Compared with transparent lenses, KCNQ1OT1, TUG1, and H19 has identified to have signifi-cantly increased expression in age-related cataract [36] [37] [38].…”