Long noncoding RNA GAS5 does not regulate HBV replication

Feng, Shu; Ji, Gaili; Ma, Jie; Wang, Zhonghao; Zhao, Yanhua; Tao, Chuanmin

doi:10.1002/jmv.25547

Cited by 7 publications

(7 citation statements)

References 38 publications

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“…In our study, GAS5 expression was significantly down-regulated in CHBV patients compared to controls. Our results were consistent with Feng et al, who reported down-regulation of GAS5 in patients with CHBV when compared to healthy controls 18 . Furthermore, in a study that identified the lncRNAs expression patterns in CHBV, GAS5 was down-regulated as compared to the healthy controls.…”

Section: Discussionsupporting

confidence: 93%

“…It is worth noting that both IFN‐α and IFN‐λ levels remained low during HCV infection after GAS5 overexpression, revealing the ability of GAS5 to inhibit innate immune responses after viral infection 18 .…”

Section: Discussionmentioning

confidence: 99%

“…MiRNAs, lncRNAs, and their target genes are emerging as biomarkers of many diseases 18 . Our study is the first to investigate miR-137 expression level in CHBV patients which could be considered as a probable diagnostic marker for these patients.…”

Section: Discussionmentioning

confidence: 99%

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GAS5 rs2067079 and miR-137 rs1625579 functional SNPs and risk of chronic hepatitis B virus infection among Egyptian patients

Taha

Hefzy

Shaker

et al. 2021

Sci Rep

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Hepatitis B virus (HBV) infection is a significant health issue worldwide.. We attempted to fulfill the molecular mechanisms of epigenetic and genetic factors associated with chronic HBV (CHBV). Expression levels of the lncRNA growth arrest-specific 5 (GAS5) and miR-137 and their corresponding SNPs, rs2067079 (C/T) and rs1625579 (G/T) were analyzed in 117 CHBV patients and 120 controls to investigate the probable association between these biomarkers and CHBV pathogenesis in the Egyptian population. Serum expression levels of GAS5 and miR-137 were significantly down-regulated in cases vs controls. Regarding GAS5 (rs2067079), the mutant TT genotype showed an increased risk of CHBV (p < 0.001), while the dominant CC was a protective factor (p = 0.004). Regarding miR-137 rs1625579, the mutant genotype TT was reported as a risk factor for CHBV (p < 0.001) and the normal GG genotype was a protective factor, p < 0.001. The serum GAS5 was significantly higher in the mutant TT genotype of GAS5 SNP as compared to the other genotypes (p = 0.007). Concerning miR-137 rs1625579, the mutant TT genotype was significantly associated with a lower serum expression level of miR-137 (p = 0.018). We revealed the dysregulated expression levels of GAS5 and miR-137 linked to their functioning SNPs were associated with CHBV risk and might act as potential therapeutic targets.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

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GAS5 rs2067079 and miR-137 rs1625579 functional SNPs and risk of chronic hepatitis B virus infection among Egyptian patients

Taha

Hefzy

Shaker

et al. 2021

Sci Rep

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“…To further confirm the role of LINC01431 against HBV in vivo, LINC01431‐PT3EF1 α plasmid was injected into HBV carrier mice prepared by pAAV‐HBV1.2 hydrodynamic injection (HDI), the nucleotide analogues Entecavir (ETV) was selected as a positive control, and lncRNA GAS5, which showed no role in HBV replication, [ 26 ] as a negative control (Figure 2D and Figure S2D, Supporting Information). As expected, compared with GAS5, LINC01431 overexpression significantly reduced the serum levels of HBsAg and HBV DNA, as well as hepatic HBcAg expression, while ETV treatment significantly decreased the levels of serum HBV‐DNA but not HBsAg (Figure 2E,F and Figure S2E, Supporting Information).…”

Section: Resultsmentioning

confidence: 99%

LINC01431 Promotes Histone H4R3 Methylation to Impede HBV Covalently Closed Circular DNA Transcription by Stabilizing PRMT1

et al. 2022

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Covalently closed circular DNA (cccDNA) is the transcriptional template of hepatitis B virus (HBV), which interacts with both host and viral proteins to form minichromosome in the nucleus and is resistant to antiviral agents. Identification of host factors involved in cccDNA transcriptional regulation is expected to prove a new venue for HBV therapy. Recent evidence suggests the involvement of long noncoding RNAs (lncRNAs) in mediating the interaction of host factors with various viruses, however, lncRNAs that HBV targets and represses cccDNA transcription have not been fully elucidated. Here, the authors identified LINC01431 as a novel host restriction factor for HBV transcription. Mechanically, LINC01431 competitively bound with type I protein arginine methyltransferase (PRMT1) to block the HBx-mediated PRMT1 ubiquitination and degradation. Consequently, LINC01431 increased the occupancy of PRMT1 on cccDNA, leading to enhanced H4R3me2a modification and reduced acetylation of cccDNA-bound histones, thereby repressing cccDNA transcription. In turn, to facilitate viral replication, HBV transcriptionally repressed LINC01431 expression by HBx-mediated repression of transcription factor Zinc fingers and homeoboxes 2 (ZHX2). Collectively, the study demonstrates LINC01431 as a novel epigenetic regulator of cccDNA minichromosome and highlights a feedback loop of HBx-LINC01431-PRMT1 in HBV replication, which provides potential therapeutic targets for HBV treatment.

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“…The rate of HCV infection prevalence varies between 0.1% -12% and is country dependent 5 . Around 350 million people were living with chronic HBV infection (8)(9)(10) and about 160 million people with HCV 4 and other declared 170 million people with CHCV infection in 2002 worldwide 8 . In 2009 figure reached 200 million which made 3.3% of the global population 4 .…”

Section: Introductionmentioning

confidence: 99%

Undiagnosed Hepatitis B and C Virus Infection at a Teaching Hospital in Rawalpindi

Khan¹,

Kalsoom²,

Batool³

et al. 2020

J Pure Appl Microbiol

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Uncontrolled prevalence of hepatitis B and C virus infections is known to be a global health problem. Many cases are undiagnosed yet. So, this study focusses on the undiagnosed existence of viral infection of hepatitis B (HBV) and Hepatitis C infection (HCV) in hospitalized patients and outdoor attendees to draw a more accurate picture of the prevalence of HBV and HCV. A cross-sectional observational study at Benazir Bhutto hospital Rawalpindi Pakistan continued for seven months and included hospitalized patients and outdoor attendees. Venous blood collected from 2003 subjects and processed to investigate infection by ELISA. We observed the overall prevalence of HBV was 2.6% and HCV was 10.98%. Further 4.2% was the highest infection rate of HBV in patients of Surgery and Medicine and the lowest in Orthopedics, Urology, Gynecology and Obstetrics, Pediatrics, Intensive Care Unit, Ear Throat and Nose, Eye>0.001%. We found the highest rate of HCV infection was in Medicine 22.90% and the lowest was in Pediatrics>0.001%. For the age, the HBV infection was found to be 4.55% the most prevalent in the age range of 61-75 and the lowest>0.001% in 76-90 years. Age group 46-60 years showed the highest 19.07% and 16-30 years the lowest 6.44% prevalence of HCV infection. Observed HBV-positive participants were 60.78% males and 39.22% females. While among the participants, males contributed 50.90% H C Virus infections and females contributed 49.10% infections. Although undiagnosed but prevalence of hepatitis B and C virus infection is very high. Therefore, some effective strategies should be implemented.

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Long noncoding RNA GAS5 does not regulate HBV replication

Cited by 7 publications

References 38 publications

GAS5 rs2067079 and miR-137 rs1625579 functional SNPs and risk of chronic hepatitis B virus infection among Egyptian patients

GAS5 rs2067079 and miR-137 rs1625579 functional SNPs and risk of chronic hepatitis B virus infection among Egyptian patients

LINC01431 Promotes Histone H4R3 Methylation to Impede HBV Covalently Closed Circular DNA Transcription by Stabilizing PRMT1

Undiagnosed Hepatitis B and C Virus Infection at a Teaching Hospital in Rawalpindi

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