Long noncoding RNA HOST2, working as a competitive endogenous RNA, promotes STAT3-mediated cell proliferation and migration via decoying of let-7b in triple-negative breast cancer

Abstract

Background: Human ovarian cancer specific transcript 2 (HOST2) is a long non-coding RNA (lncRNA) reported to be specifically high expressed in human ovarian cancer. However, the mechanism that how HOST2 regulates triple negative breast cancer (TNBC) need to be explored. Methods: In this study, expression of HOST2 was determined in 40 TNBC patients and matched non-cancerous tissues by qRT-PCR and in situ hybridization (ISH) assay. The biological functions of HOST2 was measured by losing features. The effect of HOST2 on viability, proliferation and migration was evaluated by MTT, colony formation assay, EDU analysis, transwell invasion assay and nude mouse xenograft model. Fluorescence in situ hybridization (FISH), Luciferase report assay, RNA immunoprecipitation (RIP) assay and Western blot were fulfilled to measure molecular mechanisms. Results: The results showed that HOST2 was up-regulated in BC tissues and cell lines. Clinical outcome analysis demonstrated that high expression of HOST2 was associated with poor prognosis of TNBC patients. Functional experiments illustrated that knockdown of HOST2 significantly suppressed TNBC cell proliferation and migration. Western blot assays, qRT-PCR assays, RIP assays and luciferase reporter assays revealed that HOST2 regulated STAT3 via crosstalk with let-7b. Depression of HOST2 suppressed STAT3-mediated proliferation and migration in TNBC cells. HOST2 could function as a decoy of let-7b to depress expression of STAT3. Conclusions: HOST2 could function as a oncogene and promoted STAT3-mediated proliferation and migration through acting as a competing endogenous RNA, which might act as a potential biomarker for TNBC patients.

The lncRNA H19 binding to let‐7b promotes hippocampal glial cell activation and epileptic seizures by targeting Stat3 in a rat model of temporal lobe epilepsy

“…20 In this study, TargetScan prediction and dual-luciferase reporter gene assays confirmed that Stat3 was one of the target genes of let-7b, which is consistent with previous reports. 60 We also found that Stat3 and c-Myc were markedly increased in the However, the limitation of this study was that we did not use continuous video monitoring combined with EEG recording to F I G U R E 8 Let-7b alleviated the frequency and severity of epileptic seizures in a KA-induced rat model. A, Experimental timeline.…”

ceRNA network development and tumour-infiltrating immune cell analysis of metastatic breast cancer to bone

“…LncRNA is a type of ncRNA with over 200 nucleotides, which is not related to protein-coding [17] , [18] , [19] , [20] , [21] . New evidence suggests that lncRNA imbalance occurs frequently in many malignant tumours, which is a key factor for carcinogenesis through post-transcriptional regulation and epigenetic modification [22] , [23] .…”

Prognostic value of long non-coding RNAs in triple negative breast cancer

“…The 27 relevant lncRNAs were as follows: LINC00096, [ 11 ] SNHG12, [ 12 ] TCONS_l2_00002973, [ 13 ] MALAT1, [ 14 , 15 ] KDM5B, [ 16 ] ANRIL, [ 17 ] AFAP1-AS1, [ 18 ] ARNILA, [ 19 ] AWPPH, [ 20 ] DANCR, [ 21 , 22 ] GAS5, [ 23 , 24 ] ZEB2-AS1, [ 23 , 24 ] ADPGK-AS1, [ 26 ] POU3F3, [ 27 ] HIF1A-AS2, [ 28 ] MAPT-AS1, [ 29 ] NEF, [ 30 ] LUCAT1, [ 31 ] LINC00511, [ 32 ] HOTAIR, [ 33 , 34 ] MIR503HG, [ 35 ] LncKLHDC7B, [ 36 ] HULC, [ 37 ] linc-ZNF469–3, [ 38 ] NAMPT-AS, [ 39 ] LINC00173, [ 40 ] and HOST2. [ 41 ] Among the included studies, 29 studies were conducted in China, while the other 2 were performed in Mexico and Italy, respectively. 6 studies reported HRs directly.…”