Long noncoding RNA-HOTAIR affects chemoresistance by regulating HOXA1 methylation in small cell lung cancer cells

Fang, Shun; Gao, Hong-Yi; Tong, Yue; Yang, Jie; Tang, Ruixiang; Niu, Yucun; Li, Man; Guo, Linlang

doi:10.1038/labinvest.2015.123

Cited by 99 publications

(75 citation statements)

References 27 publications

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“…Furthermore, the methylation, and transcription of PCDH10 were restored by pcDNA-HOTAIR in GIST cells previously treated with siRHOTAIRs. Our findings were supported by recent papers caliming that HOTAIR can modulate the methylation of target genes [17–20]. …”

Section: Discussionsupporting

confidence: 88%

Promoter methylation of PCDH10 by HOTAIR regulates the progression of gastrointestinal stromal tumors

Lee

Kim

et al. 2016

Oncotarget

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HOTAIR, a long non-coding RNA (lncRNA), plays a crucial role in tumor initiation and metastasis by interacting with the PRC2 complex and the modulation of its target genes. The role of HOTAIR in gastrointestinal stromal tumors (GISTs) is remains unclear. Herein we investigate the mechanism of HOTAIR in the genesis and promotion of GISTs. The expression of HOTAIR was found to be higher in surgically resected high-risk GISTs than that in low- and intermediate-risk GISTs. Using GIST-T1 and GIST882 cells, we demonstrated that HOTAIR repressed apoptosis, was associated with cell cycle progression, and controlled the invasion and migration of GIST cells. Using a gene expression microarray and lists of HOTAIR-associated candidate genes, we suggested that protocadherin 10 (PCDH10) is a key molecule. PCDH10 expression was significantly decreased in GIST-T1 and GIST882 cells, possibly as a consequence of hypermethylation. We observed that HOTAIR induced PCDH10 methylation in a SUZ12-dependent manner. In this study, we found that the malignant character of GISTs was initiated and amplified by PCDH10 in a process regulated by HOTAIR. In summary, our findings imply that PCDH10 and HOTAIR may be useful markers of disease progression and therapeutic targets.

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Section: Discussionsupporting

confidence: 88%

Promoter methylation of PCDH10 by HOTAIR regulates the progression of gastrointestinal stromal tumors

Lee

Kim

et al. 2016

Oncotarget

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“…In addition, studies showed that the long non-coding RNA HOTTIP promotes gemcitabine resistance by regulating HOXA13 in pancreatic cancer [14]. Our laboratory also reported that lncRNA HOTAIR affects chemoresistance by regulating HOXA1 methylation in SCLC [18]. However, functional roles of lncRNAs in SCLC have not been well documented.…”

Section: Introductionmentioning

confidence: 99%

Long non-coding RNA TUG1 is involved in cell growth and chemoresistance of small cell lung cancer by regulating LIMK2b via EZH2

et al. 2017

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BackgroundTaurine upregulated gene1 (TUG1) as a 7.1-kb lncRNA, has been shown to play an oncogenic role in various cancers. However, the biological functions of lncRNA TUG1 in small cell lung cancer (SCLC) remain unknown. The aim of this study is to explore the roles of TUG1 in cell growth and chemoresistance of SCLC and its possible molecular mechanism.MethodsThe expression of TUG1 in thirty-three cases of SCLC tissues and SCLC cell line were examined by quantitative RT-PCR (qRT-PCR). The functional roles of TUG1 in SCLC were demonstrated by CCK8 assay, colony formation assay, wound healing assay and transwell assay, flow cytometry analysis and in vivo study through siRNA or shRNA mediated knockdown. Western blot assays were used to evaluate gene and protein expression in cell lines. Chromatin immunoprecipitation (ChIP) and RNA binding protein immunoprecipitation (RIP) were performed to confirm the molecular mechanism of TUG1 involved in cell growth and chemoresistance of small cell lung cancer.ResultsWe found that TUG1 was overexpressed in SCLC tissues, and its expression was correlated with the clinical stage and the shorter survival time of SCLC patients. Moreover, downregulation of TUG1 expression could impair cell proliferation and increased cell sensitivity to anticancer drugs both in vitro and in vivo. We also discovered that TUG1 knockdown significantly promoted cell apoptosis and cell cycle arrest, and inhibited cell migration and invasion in vitro . We further demonstrated that TUG1 can regulate the expression of LIMK2b (a splice variant of LIM-kinase 2) via binding with enhancer of zeste homolog 2 (EZH2), and then promoted cell growth and chemoresistance of SCLC.ConclusionsTogether, these results suggested that TUG1 mediates cell growth and chemoresistance of SCLC by regulating LIMK2b via EZH2.Electronic supplementary materialThe online version of this article (doi:10.1186/s12943-016-0575-6) contains supplementary material, which is available to authorized users.

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“…Fang et al testified HOTAIR recruited HOXA1 by RNA immunoprecipitation. HOTAIR silencing reduced methylation of HOXA1, and enhanced the sensitivity of cancer cells to anticancer drugs in SCLC [88]. …”

Section: Long Non-coding Rna In Cancer Cell Drug Resistancementioning

confidence: 99%

Long non-coding RNAs in anti-cancer drug resistance

et al. 2016

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Chemotherapy is one of the basic treatments for cancers; however, drug resistance is mainly responsible for the failure of clinical treatment. The mechanism of drug resistance is complicated because of interaction among various factors including drug efflux, DNA damage repair, apoptosis and targets mutation. Long non-coding RNAs (lncRNAs) have been a focus of research in the field of bioscience, and the latest studies have revealed that lncRNAs play essential roles in drug resistance in breast cancer, gastric cancer and lung cancer, et al. Dysregulation of multiple targets and pathways by lncRNAs results in the occurrence of chemoresistance. In this review, we will discuss the mechanisms underlying lncRNA-mediated resistance to chemotherapy and the therapeutic potential of lncRNAs in future cancer treatment.

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Long noncoding RNA-HOTAIR affects chemoresistance by regulating HOXA1 methylation in small cell lung cancer cells

Cited by 99 publications

References 27 publications

Promoter methylation of PCDH10 by HOTAIR regulates the progression of gastrointestinal stromal tumors

Promoter methylation of PCDH10 by HOTAIR regulates the progression of gastrointestinal stromal tumors

Long non-coding RNA TUG1 is involved in cell growth and chemoresistance of small cell lung cancer by regulating LIMK2b via EZH2

Long non-coding RNAs in anti-cancer drug resistance

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