Long noncoding RNA<i>lncMREF</i>promotes myogenic differentiation and muscle regeneration by interacting with the Smarca5/p300 complex

Lv, Wei; Jiang, Wei; Luo, Hongmei; Tong, Qian; Niu, Xiaoyu; Liu, Xiao; Miao, Yang; Wang, Jingnan; Guo, Yiwen; Li, Jianan; Zhan, Xizhen; Hou, Yunqing; Peng, Yanjie; Wang, Jian; Zhao, Shuhong; Xu, Zaiyan; Zuo, Bo

doi:10.1093/nar/gkac854

Cited by 24 publications

(7 citation statements)

References 83 publications

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“…A few studies have undertaken global analyses of lncRNAs in regeneration, for instance following injury in skeletal muscle (Gonçalves et al 2017) or during cardiac regeneration in zebrafish (Lumley et al 2021). Specific lncRNAs have also been linked to regeneration: H19 is associated with nerve degeneration and regeneration in rats (Li et al 2022), and mucosal regeneration in mice (Geng et al 2018); lncMREF is a positive regulator of muscle regeneration in mice, pigs and humans (Lv et al 2022); CR46040 has recently been identified as crucial for the proliferation of intestinal stem cells in response to injury in Drosophila (Xu et al 2023). In the context of wing regeneration, we have identified a subset of 131 DE lncRNAs that could participate in the recovery process.…”

Section: Discussionmentioning

confidence: 99%

Expression dynamics of long non-coding RNAs during imaginal disc development and regeneration inDrosophila

Camilleri-Robles,

Amador,

Tiebe

et al. 2024

Preprint

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The discovery of functional long non-coding RNAs (lncRNAs) changed their initial concept as transcriptional noise. LncRNAs have been found to participate in the regulation of multiple biological processes, including chromatin structure, gene expression, splicing, and mRNA degradation and translation. However, functional studies of lncRNAs are hindered by the usual lack of phenotypes upon deletion or inhibition. Here, we usedDrosophilaimaginal discs as a model system to identify lncRNAs involved in development and regeneration. We examined a subset of lncRNAs expressed in the wing, leg, and eye disc development. Additionally, we analyzed transcriptomic data from regenerating wing discs to profile the expression pattern of lncRNAs during tissue repair. We focused on the lncRNACR40469, which is upregulated during regeneration. We generatedCR40469mutant flies that developed normally but showed impaired wing regeneration upon the induction of cell death. The ability of these mutants to regenerate was restored by the ectopic expression ofCR40469. Furthermore, we found that the lncRNACR34335has a high degree of sequence similarity withCR40469and can partially compensate for its function during regeneration in the absence ofCR40469. Our findings point to a potential role of the lncRNACR40469intransduring the response to damage in the wing imaginal disc.

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Section: Discussionmentioning

confidence: 99%

Expression dynamics of long non-coding RNAs during imaginal disc development and regeneration inDrosophila

Camilleri-Robles,

Amador,

Tiebe

et al. 2024

Preprint

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“…As studied, lentiviral infection is performed to deliver target transgenes to 293T cells with the robust expression of stable cell lines [21]. In this study, we developed the lentivirus in 293T cells using three vectors: 2.1 µg pLV3-CMV-MCS-3×FLAG-CopGFP-Puro or pLV3-CMV-SATB2 (pig)-3×FLAG-CopGFP-Puro, 1.4 µg psPAX2 (MiaoLingBio, Wuhan, China), and 0.7 µg PDM2.G (Addgene, Watertown, MA, USA).…”

Section: Lentivirus Packagingmentioning

confidence: 99%

Transcription Factor SATB2 Regulates Skeletal Muscle Cell Proliferation and Migration via HDAC4 in Pigs

Li,

Yan,

Yao

et al. 2024

Genes

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Skeletal muscle development remarkably affects meat production and growth rate, regulated by complex regulatory mechanisms in pigs. Specific AT sequence-binding protein 2 (SATB2) is a classic transcription factor and chromatin organizer, which holds a profound effect in the regulation of chromatin remodeling. However, the regulation role of SATB2 concerning skeletal muscle cell fate through chromatin remodeling in pigs remains largely unknown. Here, we observed that SATB2 was expressed higher in the lean-type compared to the obese-type pigs, which also enriched the pathways of skeletal muscle development, chromatin organization, and histone modification. Functionally, knockdown SATB2 led to decreases in the proliferation and migration markers at the mRNA and protein expression levels, respectively, while overexpression SATB2 had the opposite effects. Further, we found histone deacetylase 4 (HDAC4) was a key downstream target gene of SATB2 related to chromatin remodeling. The binding relationship between SATB2 and HDAC4 was confirmed by a dual-luciferase reporter system and ChIP-qPCR analysis. Besides, we revealed that HDAC4 promoted the skeletal muscle cell proliferation and migration at the mRNA and protein expression levels, respectively. In conclusion, our study indicates that transcription factor SATB2 binding to HDAC4 positively contributes to skeletal muscle cell proliferation and migration, which might mediate the chromatin remodeling to influence myogenesis in pigs. This study develops a novel insight into understanding the molecular regulatory mechanism of myogenesis, and provides a promising gene for genetic breeding in pigs.

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“…The 3′-UTRs contain important elements, such as hexameric PAS and cytoplasmic polyadenylation elements (CPE), which regulate cytoplasmic polyadenylation and interact with post-transcriptional factors to influence mRNA degradation and translation [ 21 , 22 ]. APA regulates gene expression and function in a cell- and tissue-specific manner [ 10 , 23 ], and it has been demonstrated that long non-coding RNAs (lncRNAs) exert impacts pig traits [ 24 ], especially those related to pig reproductive traits [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

Deciphering Estrus Expression in Gilts: The Role of Alternative Polyadenylation and LincRNAs in Reproductive Transcriptomics

Liu,

Chen,

Zhang

et al. 2024

Animals

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The fertility rate and litter size of female pigs are critically affected by the expression of estrus. The objective of this study was to elucidate the regulatory mechanisms of estrus expression by analyzing the differential expression of genes and long intergenic non-coding RNAs (lincRNA), as well as the utilization of alternative polyadenylation (APA) sites, in the vulva and vagina during the estrus and diestrus stages of Large White and indigenous Chinese Mi gilts. Our study revealed that the number of differentially expressed genes (DEG) in the vulva was less than that in the vagina, and the DEGs in the vulva were enriched in pathways such as “neural” pathways and steroid hormone responses, including the “Calcium signaling pathway” and “Oxytocin signaling pathway”. The DEGs in the vagina were enriched in the “Metabolic pathways” and “VEGF signaling pathway”. Furthermore, 27 and 21 differentially expressed lincRNAs (DEL), whose target genes were enriched in the “Endocrine resistance” pathway, were identified in the vulva and vagina, respectively. Additionally, we observed that 63 and 618 transcripts of the 3′-untranslated region (3′-UTR) were lengthened during estrus in the vulva and vagina, respectively. Interestingly, the genes undergoing APA events in the vulva exhibited species-specific enrichment in neural or steroid-related pathways, whereas those in the vagina were enriched in apoptosis or autophagy-related pathways. Further bioinformatic analysis of these lengthened 3′-UTRs revealed the presence of multiple miRNAs binding sites and cytoplasmic polyadenylation element (CPE) regulatory aspects. In particular, we identified more than 10 CPEs in the validated lengthened 3′-UTRs of the NFIX, PCNX4, CEP162 and ABHD2 genes using RT-qPCR. These findings demonstrated the involvement of APA and lincRNAs in the regulation of estrus expression in female pigs, providing new insights into the molecular mechanisms underlying estrus expression in pigs.

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Long noncoding RNAlncMREFpromotes myogenic differentiation and muscle regeneration by interacting with the Smarca5/p300 complex

Cited by 24 publications

References 83 publications

Expression dynamics of long non-coding RNAs during imaginal disc development and regeneration inDrosophila

Expression dynamics of long non-coding RNAs during imaginal disc development and regeneration inDrosophila

Transcription Factor SATB2 Regulates Skeletal Muscle Cell Proliferation and Migration via HDAC4 in Pigs

Deciphering Estrus Expression in Gilts: The Role of Alternative Polyadenylation and LincRNAs in Reproductive Transcriptomics

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