Long noncoding RNA LINC00342 promotes growth of infantile hemangioma by sponging miR-3619-5p from HDGF

Abstract

Infantile hemangiomas (IH) are a type of benign vascular neoplasm that may cause permanent scarring. Hemangioma-derived endothelial cells (HemECs) are commonly used as an in vitro model to study IH. Long noncoding RNA is a type of RNA transcript longer than 200 nucleotides that does not encode any protein. LINC00342 was discovered to regulate proliferation and apoptosis in nonsmall cell lung cancer. However, the role of LINC00342 in IH has never been reported before. Expressions of LINC00342 and miR-3619-5p were detected in proliferating versus normal skin tissues. Colony formation and Cell-Couting Kit 8 assays were carried out to study the effects on cell proliferation after knockdown and overexpression of LINC00342, respectively. Meanwhile caspase-3 activity and nucleosomal fragmentation assay were applied to detect cell apoptosis. Micro-RNA binding sites on LINC00342 and hepatoma-derived growth factor (HDGF) were predicted and confirmed via dual-luciferase reporter assay. Biotin RNA pulldown assay was used to verify the direct binding between RNA molecules. LINC00342 enhanced proliferation and inhibited apoptosis in HemECs. MiR-3619-5p targeted both LINC00342 and HDGF, where LINC00342 sponged miR-3619-5p and positively regulated HDGF. HDGF knockdown rescued the effects of LINC00342 on HemECs. The LINC00342-miR-3619-5p-HDGF signaling pathway could regulate cell proliferation and apoptosis in HemECs. NEW & NOTEWORTHY The role of LINC00342 in infantile hemangiomas has not yet been elucidated. This paper highlights the regulatory role of LINC00342 in cell proliferation and apoptosis in hemangioma-derived endothelial cells and the underlying molecular mechanisms. The findings would provide potential target for treatment of infantile hemangiomas.

Linc‐ROR promotes the progression of breast cancer and decreases the sensitivity to rapamycin through miR‐194‐3p targeting MECP2

“…In addition, we found that linc-ROR could function as a ceRNA sponge for miR-194-3p, miR-3164, miR-3619-5p, miR-491-5p, and miR-5001-5p. Among these five miRNAs, miR-3164 was found to participate in the ceRNA mechanism of circRAD18 to promote tumor progression in triple-negative breast cancer (Zou et al, 2019); miR-3619-5p was reported to be involved in the ceRNA regulatory networks of linc01410 in papillary thyroid carcinoma (Wang et al, 2019), linc00342 in infantile hemangioma (Liu et al, 2019), linc00202 in retinoblastoma (Yan et al, 2019), and circZFR in hepatocellular carcinoma ; miR-491-5p was found to be an inhibitor both in ERa-positive breast cancer (Hui et al, 2015) and in HER2-positive breast cancer cells (Leivonen et al, 2014). There were limited studies about miR-5001-5p in breast cancer.…”

TCONS_00012883 promotes proliferation and metastasis via DDX3/YY1/MMP1/PI3K‐AKT axis in colorectal cancer

“…Accumulating evidence indicates that lncRNAs play a crucial role in human diseases, especially in various cancers. [17][18][19][20][21][22][23][24][25][26][27][28] LncRNAs participate in almost all cellular sh-TP-53, sh-ESR1, and sh-NC. D, DDX3 and YY1 coimmunoprecipitation and western blot assay were conducted in DLD-1 cells, which transfected the overexpression of TCONS_00012883 and controls.…”

LncRNA LINC00342 contributes to the growth and metastasis of colorectal cancer via targeting miR-19a-3p/NPEPL1

“…LINC00342, a novel lncRNA located on chromosome 2q11.1, is up-regulated in non-small cell lung cancer (NSCLC) and infantile hemangiomas [24,25]. High expression of LINC00342 predicts a poor prognosis in NSCLC [26].…”