Long Noncoding RNA LINC0086 Functions as a Tumor Suppressor in Nasopharyngeal Carcinoma by Targeting miR-214

Abstract

Nasopharyngeal carcinoma (NPC) is a distinct head and neck cancer, which is occurring at a high frequency in Southern China. Emerging studies have shown that long noncoding RNAs (lncRNAs) play a critical role in carcinogenesis and progression. In this study, we established a comprehensive lncRNA profile in NPC and found that 35 lncRNAs were differentially expressed in NPC. We found that LINC0086 was decreased in NPC patient serum samples and tissues. The Kaplan-Meier survival curve showed that patients with high LINC0086 expression had a higher survival rate than those with low LINC0086 expression. LINC0086 expression was associated with NPC histological grade, lymph node metastasis, and clinical stage. Upregulation of LINC0086 inhibited cancer cell proliferation and promoted apoptosis. In addition, upregulation of LINC0086 dramatically decreased the expression of miR-214, an oncogene in several cancers, in C666-1 and HK-1 cells. An miR-214 binding site was found in the 3'-UTR of LINC0086. We also validated that both miR-214 and LINC0086 presented in the RISC complex, demonstrating that LINC0086 could decrease miR-214 expression by directly interacting with miR-214. Furthermore, the suppressive effects of LINC0086 on NPC cell growth were reversed by overexpression of miR-214 in vitro and in vivo. Thus, our study reports a novel mechanism underlying NPC carcinogenesis and provides a potential novel diagnosis and treatment biomarker for NPC.

HNF1A-AS1 promotes growth and metastasis of esophageal squamous cell carcinoma by sponging miR-214 to upregulate the expression of SOX-4

“…Others reported that miR-214 mediated the inhibiting effect of FOXD3 on proliferation, invasion and metastasis by targeting MED19 in colon cancer (44). Moreover, miR-214 acted as a target of LncR-LINC0086 and overexpressed miR-214 reversing the suppressive effects of LINC0086 on nasopharyngeal carcinoma (NPC) growth in vitro and in vivo (45). Generally, miRNA is regulated by the upstream transcription factors.…”

Long non‐coding RNA SNHG15 promotes CDK14 expression via miR‐486 to accelerate non‐small cell lung cancer cells progression and metastasis

“…Emerging evidence have shown that lncRNAs function as oncogenic or suppressor genes in multiple cancers, involving in transcription and post‐transcription regulation (Guo, Ma et al, ; He et al, ). In present study, our team performed series of experiments to investigate the biological role of lncRNA SNHG15 on non‐small‐cell lung cancer (NSCLC) tumorigenesis.…”

Functions and Roles of Long-Non-Coding RNAs in Human Nasopharyngeal Carcinoma

“…LINC0086 showed to decrease in miR-214 expression by directly interacting with miR-214 in the 3’-UTR region. Furthermore, the reduced LINC0086 on cell growth of NPC were reversed by exogenously expressed miR-214 in vitro and in vivo suggesting a potential new diagnostic and therapeutic biomarker for NPC [59]. LOC401317 was highly induced by p53 transgene expression.…”

“…These observations indicated a pivotal role for lncRNA-LET in NPC cell proliferation. The expression of lncRNA LINC0086 was also reduced in NPC, resulting in increased proliferation and decreased apoptosis in NPC cells [59]. LINC0086q expression was associated with histological grades, lymph node metastasis, and clinical stages in patients with NPC.…”