Long noncoding RNA NBAT-1 suppresses tumorigenesis and predicts favorable prognosis in ovarian cancer

Abstract

Long noncoding RNA (lncRNA) has been proven to be involved in many biological processes in ovarian cancer (OC). However, the mechanism still remains unknown. In this study, we screened significantly downregulated NBAT-1, which has been proven to play a significant role in breast cancer, clear cell renal cell carcinoma, and neuroblastoma, but not in OC, in two independent datasets with relatively more samples (GSE18520 and GSE38666) from Gene Expression Omnibus. We found that lncRNA NBAT-1 was obviously downregulated in OC tissue compared to normal ovarian tissue (P<0.001) which was free of OC, and the detected levels of NBAT-1 were associated with the International Federation of Gynecology and Obstetrics stage and tumor size guidelines. Moreover, it has been shown that lower levels of NBAT-1 predict poor outcomes of OC. In order to investigate the functional role of NBAT-1, pcDNA-NBAT-1 and empty vector were transfected into TOV112D and OVCAR-3 cell lines. Overexpressed NBAT-1 significantly inhibited cell proliferation, invasion, and migration in both TOV112D and OVCAR-3 cell lines. Finally, Western blot assay indicated that NBAT-1 may exert its function by targeting the ERK1/2 and AKT signaling pathways. In addition, tumor formation growth assay showed that overexpressed NBAT-1 significantly suppresses tumor growth in vivo. In conclusion, our study suggests that NBAT-1 acts as an anti-oncogene in the development of OC.

Altered long noncoding RNAs and survival outcomes in ovarian cancer

“…Finally, 15 studies that met our eligibility criteria were included in the meta-analysis. [ 14 , 34 – 47 ] The flow diagram summarizing the process of study selection is given in Fig. 1 .…”

LncRNA NBAT1 suppresses cell proliferation and migration via miR‐346/GSK‐3β axis in renal carcinoma

“…NBAT1 was initially downregulated in high‐risk neuroblastomas . The anticarcinogenic effects of NBAT1 has been reported in solid tumors, such as ovarian cancer (OC), glioblastoma, gastric cancer (GC) and non‐small cell lung cancer (NSCLC) . Identifying the downstream targets of NBAT1 will elucidate its critical role in RCC progression.…”

Long non‑coding RNA CASC2 inhibits progression and predicts favorable prognosis in epithelial ovarian cancer

“…Numerous lncRNAs are involved in regulating EOC biology through various ways. In addition, certain lncRNAs, including AB073614 (32), colon cancer associated transcript 2 (33) and neuroblastoma associated transcript 1 (34), have been demonstrated to be potential prognostic markers in patients with EOC. However, the significance of CASC2 in EOC has not been elucidated yet.…”