Long noncoding RNA TSLNC8 suppresses cell proliferation and metastasis and promotes cell apoptosis in human glioma

Abstract

Glioma is among the most common primary brain tumors and one of the most aggressive and lethal forms of human cancer. Long noncoding RNAs (lncRNAs) have demonstrated great importance in the development and progression of cancer. The present study aimed to investigate the role of the novel tumor suppressive lncRNA on Chromosome 8p12 (TSLNC8), in cell proliferation, metastasis and apoptosis in human glioma. It was initially reported that the relative transcript levels of TSLNC8 were significantly decreased in human glioma tissues and cultured glioma cells, as evidenced by RT-qPCR. Among clinical variables, the expression of TSLNC8 was negatively associated with tumor size, distant metastasis, and tumor, node and metastasis stage. MTT assay demonstrated that overexpression of TSLNC8 in glioma cell lines U25-MG and SWO38 decreased, whereas knockdown of TSLNC8 in glioma cells SHG-44 and BT325 increased the cell proliferative rate over 5 consecutive days. Additionally, cell metastasis was inhibited in U251 and SWO38 cells when cells were transfected with TSLNC8-expressing plasmid as observed via Transwell and wound-healing assays. Furthermore, cell apoptotic rate was upregulated in TSLNC8 plasmid-treated U251 and SWO38 cells, and inhibited by siRNA against TSLNC8 in SHG-44 and BT325 cells by cell apoptotic assay. The relative activities of caspase-3 and caspase-9 were increased by TSLNC8 overexpression and decreased by TSLNC depletion; however, the activity of caspase-8 remained unchanged. The results of the present study demonstrated the inhibitory effects of TSLNC8 in human glioma, which may contribute to advancement in the diagnosis and treatment of patients with glioma in clinic.

Interaction of BACH2 with FUS promotes malignant progression of glioma cells via the TSLNC8–miR‐10b‐5p–WWC3 pathway

“…Our study showed that TSLNC8 was downregulated in glioma tissues and cells. TSLNC8 overexpression significantly inhibited the viability, migration and invasion of glioma cells and promoted glioma cell apoptosis, indicating that TSLNC8 acts as a tumour suppressor in glioma cells, which is consistent with the findings reported by Chen and Yu in their study [18]. However, our findings further revealed the mechanism by which TSLNC8 regulates the biological behaviour of glioma cells.…”

“…It has been reported that TSLNC8 acts as a tumour suppressor and is involved in the regulation of various malignant tumour diseases, including hepatocellular carcinoma [15], breast cancer [16] and non-small cell lung cancer [17]. Additionally, existing studies have shown that TSLNC8 suppresses cell proliferation and metastasis and promotes cell apoptosis in human glioma [18]. However, the detailed function of TSLNC8 in glioma remains largely unknown.…”

Long noncoding RNA TSLNC8 enhances pancreatic cancer aggressiveness by regulating CTNNB1 expression via association with HuR

“…TSLNC8 was rstly found to be downregulated in hepatocellular carcinoma (HCC) tissues and signi cantly suppress the proliferation and metastasis of HCC cells by competitively interacting with transketolase and STAT3 and inactivating IL-6/STAT3 pathway (11). Downregulation of TSLNC8 expression was also observed in breast cancer, glioma, and non-small cell lung cancer (12)(13)(14). However, the functional role and molecular mechanism of TSLNC8 in PC progression remain to be elucidated.…”

Long Non-Coding RNAs (lncRNAs) Tumor-Suppressive Role of lncRNA on Chromosome 8p12 (TSLNC8) Inhibits Tumor Metastasis and Promotes Apoptosis by Regulating Interleukin 6 (IL-6)/Signal Transducer and Activator of Transcription 3 (STAT3)/Hypoxia-Inducible Factor 1-alpha (HIF-1α) Signaling Pathway in Non-Small Cell Lung Cancer

“…Tumor-suppressive lncRNA on chromosome 8p12 (TSLNC8), also named LINC00589, is one of various lncRNAs [17]. TSLNC8 was reported to be remarkably downregulated in human glioma tissues, suppressing proliferation and facilitating apoptosis in human glioma cells [18]. Furthermore, TSLNC8 exerts its tumor-suppressive function by inactivating the IL-6/STAT3 signaling pathway in liver cancer [17].…”