Long noncoding <scp>RNA OIP5‐AS1</scp> promotes the stemness of lung cancer cells through enhancing Oct4 <scp>mRNA</scp> stability

Mao, Chengye; Li, Xionghui

doi:10.1002/tox.23468

Cited by 11 publications

(5 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Increasing evidence has established that regulation of OCT4 expression plays a crucial role in the regulation of CSC properties in many human malignancies. Opa interacting protein 5-antisense RNA 1 (OIP5-AS1) promotes the stemness of lung cancer cells by enhancing OCT4 mRNA stability [ 40 ]. The results demonstrated that lncRNA OIP5-AS1 can confer lung cancer CSC-like traits by directly interacting with OCT4 mRNA, thus increasing OCT4 mRNA stability and expression, and knockdown of OCT4 could rescue the promoting effects of OIP5-AS1 overexpression on CSC-like traits.…”

Section: Discussionmentioning

confidence: 99%

A positive feedback between PDIA3P1 and OCT4 promotes the cancer stem cell properties of esophageal squamous cell carcinoma

Huang,

You,

Huang

et al. 2024

Cell Commun Signal

View full text Add to dashboard Cite

Background Increasing evidence has indicated that long non-coding RNAs (lncRNAs) have been proven to regulate esophageal cancer progression. The lncRNA protein disulfide isomerase family A member 3 pseudogene 1 (PDIA3P1) has been shown to promote cancer stem cell properties; however, its mechanism of action remains unclear. In this study, we investigated the regulation of esophageal cancer stem cell properties by the interaction of PDIA3P1 with proteins. Methods The GEPIA2 and Gene Expression Omnibus databases were used to analyze gene expression. PDIA3P1 expression in human esophageal squamous cell carcinoma (ESCC) tissues and cell lines was detected by quantitative real-time polymerase chain reaction (qRT-PCR). Loss-of-function experiments were performed to determine the effects of PDIA3P1 on ESCC cell proliferation, migration, and invasion. The sphere formation assay, number of side population cells, and CD271 + /CD44 + cells were detected by flow cytometry to identify the cancer stem cell properties. RNA immunoprecipitation (RIP), RNA pull-down, co-immunoprecipitation (co-IP), dual luciferase reporter, and cleavage under targets and tagmentation (CUT&Tag) assays were performed to elucidate the underlying molecular mechanisms. Results PDIA3P1 expression was upregulated in ESCC cell lines and tissues. Functionally, higher PDIA3P1 expression promoted cell proliferation, invasion, and metastasis and inhibited apoptosis in esophageal cancer. Importantly, PDIA3P1 promoted cancer stem cell properties in ESCC. Mechanistically, PDIA3P1 interacted with and stabilized octamer-binding transcription factor 4 (OCT4) by eliminating its ubiquitination by the ubiquitinating enzyme WW domain-containing protein 2 (WWP2). Moreover, as a transcription factor, OCT4 bound to the PDIA3P1 promoter and promoted its transcription. Conclusions Our research revealed a novel mechanism by which a positive feedback loop exists between PDIA3P1 and OCT4. It also demonstrated that the PDIA3P1-WWP2-OCT4 loop is beneficial for promoting the cancer stem cell properties of ESCC. Owing to this regulatory relationship, the PDIA3P1-WWP2-OCT4-positive feedback loop might be used in the diagnosis and prognosis, as well as in the development of novel therapeutics for esophageal cancer.

show abstract

Section: Discussionmentioning

confidence: 99%

A positive feedback between PDIA3P1 and OCT4 promotes the cancer stem cell properties of esophageal squamous cell carcinoma

Huang,

You,

Huang

et al. 2024

Cell Commun Signal

View full text Add to dashboard Cite

show abstract

“…LncRNA opa‐interacting protein 5 antisense transcript 1 (OIP5‐AS1) also had a dysregulation in different cancers. Mechanically, it not only interacted with miRNAs to perform its function in ovarian cancer (OC), 66 endometrial carcinoma (EC), 67 and papillary thyroid cancer (PTC), 68 but could directly interact with Oct4 mRNA to promote its stability in lung cancer 69 . SEMA6A‐AS1 expression was decreased in HBV‐related HCC tissues compared with adjacent noncancerous tissues, and the expression level of SEMA6A‐AS1 was associated with prognosis of these patients 70 .…”

Section: Discussionmentioning

confidence: 99%

m6A‐ and m5C‐ modified lncRNAs orchestrate the prognosis in cutaneous melanoma and m6A‐ modified LINC00893 regulates cutaneous melanoma cell metastasis

Shi,

Tian,

et al. 2024

Skin Research and Technology

View full text Add to dashboard Cite

BackgroundAs the most important modifications on the RNA level, N6‐methyladenosine (m6A‐) and 5‐methylcytosine (m5C‐) modification could have a direct influence on the RNAs. Long non‐coding RNAs (lncRNAs) could also be modified by methylcytosine modification. Compared with mRNAs, the function of lncRNAs could be more potent to some extent in biological processes like tumorigenesis. Until now, rare reports have been done associated with cutaneous melanoma. Herein, we wonder if the m6A‐ and m5C‐ modified lncRNAs could influence the immune landscape and prognosis in melanoma, and we also want to find some lncRNAs which could directly affect the malignant behaviors of melanoma.MethodsSystematically, we explored the expression pattern of m6A‐ and m5C‐ modified lncRNAs in melanoma from datasets including UCSC Xena and NCBI GEO, and the prognostic lncRNAs were selected. Then, according to the expression pattern of lncRNAs, melanoma samples from these datasets were divided into several subtypes. Prognostic model, nomogram survival model, drug sensitivity, GO, and KEGG pathway analysis were performed. Furthermore, among several selected lncRNAs, we identified one lncRNA named LINC00893 and investigated its expression pattern and its biological function in melanoma cell lines.ResultsWe identified 27 m6A‐ and m5C‐ related lncRNAs which were significantly associated with survival, and we made a subtype analysis of melanoma samples based on these 27 lncRNAs. Among the two subtypes, we found differences of immune cells infiltration between these two subtypes. Then, LASSO algorithm was used to screen the optimized lncRNAs combination including ZNF252P‐AS1, MIAT, FAM13A‐AS1, LINC‐PINT, LINC00893, AGAP2‐AS1, OIP5‐AS1, and SEMA6A‐AS1. We also found that there was a significant correlation between the different risk groups predicted based on RS model and the actual prognosis. The nomogram survival model based on independent survival prognostic factors was also constructed. Besides, sensitivity to chemotherapeutic agents, GO and KEGG analysis were performed. In different risk groups, a total of 14 drug molecules with different distributions were obtained, which included AZD6482, AZD7762, AZD8055, camptothecin, dasatinib, erlotinib, gefitinib, gemcitabine, GSK269962A, nilotinib, rapamycin, and sorafenib. A total of 55 significantly related biological processes and 17 KEGG signaling pathways were screened. At last, we noticed that LINC00893 had a relatively lower expression in melanoma tissue and cell lines compared with adjacent tissues and epidermal melanocyte, and down‐regulation of LINC00893 could promote the malignant behavior of melanoma cells in A875 and MV3. In these two melanoma cell lines, down‐regulation of m6A‐related molecules like YTHDF3 and METTL3 could promote the expression of LINC00893.ConclusionWe made an analysis of m6A‐ and m5C‐ related lncRNAs in melanoma samples and a prediction of these lncRNAs’ role in prognosis, tumor microenvironment, immune infiltration, and clinicopathological features. We also found that LINC00893, which is potentially regulated by m6A modification, could serve as a tumor‐suppressor in melanoma and play an inhibitory role in melanoma metastasis.

show abstract

“…It is a key factor in gene regulation and also involved in the function of CSCs and EMT [ 105 ]. LncRNA OIP5-AS1 can directly interact with OCT4 mRNA, enhance the stability of OCT4 mRNA to increase OCT4 expression, and endow lung cancer cells with CSC characteristics [ 106 ]. The interaction between lncRNA and miRNA forms a competitive endogenous RNA (ceRNA) mode and possesses a negative feedback regulation mechanism.…”

Section: Introductionmentioning

confidence: 99%

Transcriptional regulation of cancer stem cell: regulatory factors elucidation and cancer treatment strategies

Zhang,

Zhang

2024

J Exp Clin Cancer Res

View full text Add to dashboard Cite

Cancer stem cells (CSCs) were first discovered in the 1990s, revealing the mysteries of cancer origin, migration, recurrence and drug-resistance from a new perspective. The expression of pluripotent genes and complex signal regulatory networks are significant features of CSC, also act as core factors to affect the characteristics of CSC. Transcription is a necessary link to regulate the phenotype and potential of CSC, involving chromatin environment, nucleosome occupancy, histone modification, transcription factor (TF) availability and cis-regulatory elements, which suffer from ambient pressure. Especially, the expression and activity of pluripotent TFs are deeply affected by both internal and external factors, which is the foundation of CSC transcriptional regulation in the current research framework. Growing evidence indicates that regulating epigenetic modifications to alter cancer stemness is effective, and some special promoters and enhancers can serve as targets to influence the properties of CSC. Clarifying the factors that regulate CSC transcription will assist us directly target key stem genes and TFs, or hinder CSC transcription through environmental and other related factors, in order to achieve the goal of inhibiting CSC and tumors. This paper comprehensively reviews the traditional aspects of transcriptional regulation, and explores the progress and insights of the impact on CSC transcription and status through tumor microenvironment (TME), hypoxia, metabolism and new meaningful regulatory factors in conjunction with the latest research. Finally, we present opinions on omnidirectional targeting CSCs transcription to eliminate CSCs and address tumor resistance.

show abstract

Long noncoding RNA OIP5‐AS1 promotes the stemness of lung cancer cells through enhancing Oct4 mRNA stability

Cited by 11 publications

References 20 publications

A positive feedback between PDIA3P1 and OCT4 promotes the cancer stem cell properties of esophageal squamous cell carcinoma

A positive feedback between PDIA3P1 and OCT4 promotes the cancer stem cell properties of esophageal squamous cell carcinoma

m6A‐ and m5C‐ modified lncRNAs orchestrate the prognosis in cutaneous melanoma and m6A‐ modified LINC00893 regulates cutaneous melanoma cell metastasis

Transcriptional regulation of cancer stem cell: regulatory factors elucidation and cancer treatment strategies

Contact Info

Product

Resources

About